Molecular characteristics of <i>Shigella</i> spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

Na-Ubol, Mathukorn; Samosornsuk, Seksun; Seidlein, Lorenz von; Tapchaisri, Pramuan; Ali, Mohammad; Clemens, John D.; Chaicumpa, Wanpen

doi:10.1017/s0950268806005899

Cited by 7 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After gel electrophoresis, gels were stained with ethidium bromide, rinsed, and photographed under UV light. The banding patterns of all isolates evaluated with PFGE were compared by visual inspection, and the isolates were grouped as suggested by Na-Ubol et al (2006). Isolates were defined as epidemic ones when belonging to a genotype that was identified in at least 2 individuals.…”

Section: Molecular Typingmentioning

confidence: 99%

Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007

Bao

Chen

et al. 2012

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

Shigella sonnei has become the dominant serotype causing shigellosis in Asian countries in recent years. In this study, we characterize the increasing trend of antibiotic resistance profiles and genotypes of S. sonnei isolates in the Beijing area. From January 2002 to December 2007, a total of 1108 Shigella isolates including 362 S. sonnei were recovered from diarrhea patients at the 302nd Hospital in Beijing. While the frequency of S. flexneri gradually decreased, S. sonnei gradually increased and became the dominant species. A total of 362 S. sonnei isolates were further analyzed for their antimicrobial profiles and 272 revived isolates were selected for genotyping analysis, respectively. High-level antimicrobial resistances were observed in sulfamethoxazole/trimethoprim (94.5%), ampicillin (40.3%), piperacillin (36.5%), and ceftriaxone (12.8%) with significant single- and multiple-drug resistance increase trends from 2002 to 2007 (P = 0.0000). Pulsed-field gel electrophoresis analysis indicated that 263 (96.7%) S. sonnei belonged to 1 clonal genotype A, which were further divided into A1-A6 subtypes. While subtype A2 was dominant in the early stage of study years, subtype A4 started to emerge and increased significantly in later years. Antimicrobial resistance rates are statistically different among the 6 subtypes (P = 0.0000), and A4 possessed the highest resistance rates to ampicillin (83.7%) and piperacillin (81.4%). Subtype A3 was highly clustered in inpatients compared to other subtypes (P = 0.0145). This study indicates that a clonal S. sonnei strain has become dominant in the Beijing area, and subtype A4 is responsible for increased antibiotic resistance.

show abstract

Section: Molecular Typingmentioning

confidence: 99%

Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007

Bao

Chen

et al. 2012

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

show abstract

“…Molecular typing and antimicrobial susceptibility of S. sonnei isolates can provide very useful data for the management of both isolated cases and outbreaks ( DeLappe et al , 2003 ; Liang et al , 2007 ; Na-Ubol et al , 2006 ). In this study, we investigated the rate of antimicrobial resistance of S. sonnei strains collected in Ankara, the capital city of Turkey, from 2004 to 2006 and analyzed ESBL-producing isolates by PCR and DNA sequencing.…”

mentioning

confidence: 99%

Drug resistance profiles and clonality of sporadic Shigella sonnei isolates in Ankara, Turkey

Kaçmaz

Ünaldı²,

Sultan

et al. 2014

Braz. J. Microbiol.

View full text Add to dashboard Cite

The aims of this study were to investigate drug resistance rates, types of extended spectrum beta lactamases (ESBLs), and molecular epidemiological characteristics of 43 Shigella sonnei isolates. Ampicillin-sulbactam, amoxicillin-clavulanate, chloramphenicol, and ciprofloxacin were the most active antibiotics. Five isolates harbored blaSHV-12, blaTEM-1 and blaCTX-M-15. More than 90% of the isolates had an indistinguishable pulsotype.

show abstract

“…One hundred and fifty three S. sonnei strains were isolated from patients with shigellosis in the Kaeng-Khoi district of Saraburi province during May 2000 to April 2003 (Na-ubol et al , 2006). Bacteriocin activity was screened for antibacterial activity against the indicator strain, S. sonnei .…”

Section: Methodsmentioning

confidence: 99%

Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea

Kaewklom¹,

Samosornsuk²,

Pipatsatitpong³

et al. 2013

Braz. J. Microbiol.

View full text Add to dashboard Cite

Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity of PPB from this strain to proteinase K, trypsin and α-chymotrypsin suggests the proteinaceous nature of these antimicrobial substances. Therefore, this isolated bacterium can be regarded as bacteriocin producing bacteria. The bacteriocin produced by this isolated S. sonnei was heat stable as evidenced by its ability to maintain the activity at 80 °C for 60 min. In addition, it was stable within a wide range of pH (3–9). The molecular weight of colicin type 7 from isolated S. sonnei strain 35/44 analyzed by SDS-PAGE was 54.4 kDa composing of at least five subunits. It is to our knowledge; the first report of Thai patients with diarrhoea that S. sonnei isolated from them contained colicin type 7.

show abstract

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

Abstract: Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand. Epidemiology and infection, 134 (5).

Cited by 7 publications

References 16 publications

Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007

Genotypes and antimicrobial profiles of Shigella sonnei isolates from diarrheal patients circulating in Beijing between 2002 and 2007

Drug resistance profiles and clonality of sporadic Shigella sonnei isolates in Ankara, Turkey

Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea

Contact Info

Product

Resources

About