Molecular Characteristics of IS
            <i>1216</i>
            Carrying Multidrug Resistance Gene Cluster in Serotype III/Sequence Type 19 Group B Streptococcus

Zhi, Yong; Ji, Hyun Jung; Jung, Jong Hyun; Byun, Eui Baek; Kim, Woo Sik; Lin, Shun Mei; Lim, Sangyong; Jang, A-Yeung; Choi, Min Joo; Ahn, Kyu Jeung; Lim, Jae Hyang; Song, Joon Young; Seo, Ho Seong

doi:10.1128/msphere.00543-21

Cited by 7 publications

(6 citation statements)

References 72 publications

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“…We also detected the aminoglycoside resistance gene aadE. Resistance to aminoglycosides mediated by aadE has been reported for human clinical GBS in the UK [33], South Korea [34], and China [35], and for bovine mastitis isolates belonging to the species Staphylococcus aureus [36]. To our knowledge, it has not been described in bovine GBS previously.…”

Section: Discussionmentioning

confidence: 74%

Long-Term Co-Circulation of Host-Specialist and Host-Generalist Lineages of Group B Streptococcus in Brazilian Dairy Cattle with Heterogeneous Antimicrobial Resistance Profiles

Oliveira,

Simões,

Crestani

et al. 2024

Antibiotics

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Group B Streptococcus (GBS) is a major cause of contagious bovine mastitis (CBM) in Brazil. The GBS population is composed of host-generalist and host-specialist lineages, which may differ in antimicrobial resistance (AMR) and zoonotic potential, and the surveillance of bovine GBS is crucial to developing effective CBM control and prevention measures. Here, we investigated bovine GBS isolates (n = 156) collected in Brazil between 1987 and 2021 using phenotypic testing and whole-genome sequencing to uncover the molecular epidemiology of bovine GBS. Clonal complex (CC) 61/67 was the predominant clade in the 20th century; however, it was replaced by CC91, with which it shares a most common recent ancestor, in the 21st century, despite the higher prevalence of AMR in CC61/67 than in CC91, and high selection pressure for AMR from indiscriminate antimicrobial use in the Brazilian dairy industry. CC103 also emerged as a dominant CC in the 21st century, and a considerable proportion of herds had two or more GBS strains, suggesting poor biosecurity and within-herd evolution due to the chronic nature of CBM problems. The majority of bovine GBS belonged to serotype Ia or III, which was strongly correlated with CCs. Ninety-three isolates were resistant to tetracycline (≥8 μg/mL; tetO = 57, tetM = 34 or both = 2) and forty-four were resistant to erythromycin (2.0 to >4 μg/mL; ermA = 1, ermB = 38, mechanism unidentified n = 5). Only three isolates were non-susceptible to penicillin (≥8.0 μg/mL), providing opportunities for improved antimicrobial stewardship through the use of narrow-spectrum antimicrobials for the treatment of dairy cattle. The common bovine GBS clades detected in this study have rarely been reported in humans, suggesting limited risk of interspecies transmission of GBS in Brazil. This study provides new data to support improvements to CBM and AMR control, bovine GBS vaccine design, and the management of public health risks posed by bovine GBS in Brazil.

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Section: Discussionmentioning

confidence: 74%

Long-Term Co-Circulation of Host-Specialist and Host-Generalist Lineages of Group B Streptococcus in Brazilian Dairy Cattle with Heterogeneous Antimicrobial Resistance Profiles

Oliveira,

Simões,

Crestani

et al. 2024

Antibiotics

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“…An examination of ARGs and selected virulence factors (VFs) in S. aureus sequence data showed that six ARGs were frequently found in pig farm isolates (pig farm‐associated ARG [PFA‐ARGs]), fexA (phenicols ARG), [ 29 ] lnuB (lincosamides ARG), [ 30 ] dfrG (trimethoprim ARG), [ 31 ] tetL (tetracyclines ARG), [ 32 ] aac6‐Aph2 , and spw (aminoglycosides ARGs); [ 33 , 34 ] and three VFs frequently found in hospital isolates (hospital‐associated VFs [HA‐VFs]), scn ( Staphylococcal complement inhibitor), [ 35 ] sak (staphylokinase), [ 35 ] and sprD (small pathogenicity island RNAs) [ 36 ] (Figure S2 , Supporting Information). The differences in PFA‐ARGs and HA‐VFs compared using a Fisher's exact test ( p ‐value is <0.00001) (Table S5 , Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

A Survey of Chinese Pig Farms and Human Healthcare Isolates Reveals Separate Human and Animal Methicillin‐Resistant Staphylococcus aureus Populations

et al. 2021

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There has been increasing concern that the overuse of antibiotics in livestock farming is contributing to the burden of antimicrobial resistance in people. Farmed animals inEurope and North America, particularly pigs, provide a reservoir for livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA ST398 lineage) found in people. This study is designed to investigate the contribution of MRSA from Chinese pig farms to human infection. A collection of 483 MRSA are isolated from 55 farms and 4 hospitals in central China, a high pig farming density area. CC9 MRSA accounts for 97.2% of all farm isolates, but is not present in hospital isolates. ST398 isolates are found on farms and hospitals, but none of them formed part of the "LA-MRSA ST398 lineage" present in Europe and North America. The hospital ST398 MRSA isolate form a clade that is clearly separate from the farm ST398 isolates. Despite the presence of high levels of MRSA found on Chinese pig farms, the authors find no evidence of them spilling over to the human population. Nevertheless, the ST398 MRSA obtained from hospitals appear to be part of a widely distributed lineage in China. The new animal-adapted ST398 lineage that has emerged in China is of concern.

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“…The mechanism of HLGR resistance in GBS is mediated by the chromosomally integrated transposon Tn3706 (a derivative of enterococcal Tn5281 or staphylococcal Tn4001) or plasmid pIP501 [ 12 , 14 , 29 , 30 , 34 , 35 , 36 , 37 ]. Nevertheless, the HLGR rate in GBS is rarely reported, although probably underestimated due to the absence of international guidelines on HLGR susceptibility testing for GBS.…”

Section: Discussionmentioning

confidence: 99%

“…One representative isolate analyzed by whole-genome sequencing revealed the presence of four large novel cluster sites. Specifically, site 4 in the srr1 gene locus was replaced by an lsa ( E ) -lnu ( B ) -aadK-aac-aph-aadE -carrying multidrug-resistant gene cluster flanked by two IS1216 transposases with 99% homology to the enterococcal plasmid pKUB3007-1 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Emergence of High-Level Gentamicin Resistance in Streptococcus agalactiae Hypervirulent Serotype IV ST1010 (CC452) Strains by Acquisition of a Novel Integrative and Conjugative Element

Creti,

Imperi,

Khan

et al. 2024

Antibiotics

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Streptococcus agalactiae (group B streptococci, GBS) is responsible for severe infections in both neonates and adults. Currently, empiric antimicrobial therapy for sepsis and meningitis is the combined use of penicillin and gentamicin due to the enhanced bactericidal activity. However, high-level gentamicin resistance (HLGR) abrogates the synergism. The rate of HLGR was investigated within a dataset of 433 GBS strains collected from cases of invasive disease in both adults and neonates as well as from pregnant carriers. GBS isolates (n = 20, 4.6%) presented with HLGR (gentamicin MIC breakpoint >1024 mg/L) that was differently diffused between strains from adults or neonates (5.2% vs. 2.8%). Notably, 70% of HLGR GBS strains (14 isolates) were serotype IV. Serotype IV HLGR-GBS isolates were susceptible to all antibiotics tested, exhibited the alpha-C/HvgA/PI-2b virulence string, and belonged to sequence type 1010 (clonal complex (CC) 452). The mobile element that harbored the HLGR aac(6′)-aph(2)″ gene is a novel integrative and conjugative element (ICE) about 45 kb long, derived from GBS 515 ICE tRNALys. The clonal expansion of this HLGR hypervirulent serotype IV GBS CC452 sublineage may pose a threat to the management of infections caused by this strain type.

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Molecular Characteristics of IS 1216 Carrying Multidrug Resistance Gene Cluster in Serotype III/Sequence Type 19 Group B Streptococcus

Abstract: Most previously isolated group B streptococcus (GBS) strains express either the Srr1 or Srr2 glycoprotein, which plays an important role in bacterial colonization and invasion. These glycoproteins are potential protein vaccine candidates.

Cited by 7 publications

References 72 publications

Long-Term Co-Circulation of Host-Specialist and Host-Generalist Lineages of Group B Streptococcus in Brazilian Dairy Cattle with Heterogeneous Antimicrobial Resistance Profiles

Long-Term Co-Circulation of Host-Specialist and Host-Generalist Lineages of Group B Streptococcus in Brazilian Dairy Cattle with Heterogeneous Antimicrobial Resistance Profiles

A Survey of Chinese Pig Farms and Human Healthcare Isolates Reveals Separate Human and Animal Methicillin‐Resistant Staphylococcus aureus Populations

Emergence of High-Level Gentamicin Resistance in Streptococcus agalactiae Hypervirulent Serotype IV ST1010 (CC452) Strains by Acquisition of a Novel Integrative and Conjugative Element

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