Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis

Goudarzi, Mehdi; Seyedjavadi, Sima Sadat; Nasiri, Mohammad Javad; Goudarzi, Hossein; Nia, Raheleh Sajadi; Dabiri, Hossein

doi:10.1016/j.micpath.2017.01.055

Cited by 77 publications

(95 citation statements)

References 42 publications

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“…Accurate susceptibility data are important to appropriate treatment options. In the current study, the most resistant pattern among MRSA strains was observed in betalactam antibiotics including penicillin (97.5%) followed by tetracycline (78%), kanamycin (77.5%), gentamicin (75%), erythromycin 114 (57%), amikacin (50%), and clindamycin, while antibiotics such as vancomycin, teicoplanin, and linezolid had good activity against MRSA infections and these results were largely in line with the findings of Goudarzi et al, (23) and Ko et al (24).…”

Section: Discussionsupporting

confidence: 91%

“…This finding was in line with those of Dormanesh et al (40) that showed tetK (89.18%), mecA (71.62%), msrA (56.75%), and tetM (54.05%) as the most commonly detected antibiotic resistance genes in their study. As shown in Table 4, the most prevalent toxin encoding gene was tst (61.5%), which was in agreement with those of the other studies (1,22,23). In the current study, pvl genes were detected in 21.5% of the tested isolates.…”

Section: Discussionsupporting

confidence: 91%

“…In the current study, pvl genes were detected in 21.5% of the tested isolates. Previous studies reported the prevalence of 2% to 35% of pvl genes among MRSA strains (7,23). Regarding the frequency of the exfoliative toxins, the results revealed that eta was more common (9.5%) than what was reported in Colombia (3%) (41) and the previous study in Iran (2), while it was lower than the rate reported in Turkey (19.2%) (42).…”

Section: Discussionmentioning

confidence: 55%

“…The MRSA strains are usually resistant to macrolides, lincosamides, aminoglycoside, and approximately all currently available beta-lactam antimicrobial agents such as penicillin and cephalosporins (17,22,23). Accurate susceptibility data are important to appropriate treatment options.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Genes Encoding Toxin, Adhesion, and Antibacterial Resistance Among Various SCCmec Types of Methicillin-Resistant Staphylococcus aureus Isolated From Intensive Care Unit, Tehran, Iran

Abiri

Sepahi

Goudarzi

et al. 2017

Jundishapur J Microbiol

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Background: Most methicillin-resistant Staphylococcus aureus (MRSA) infections occur in health care settings; therefore, it is important to know about antimicrobial susceptibility, and carriage of virulence genes in S. aureus isolates. Objectives: The current study aimed at determining the prevalence of genes coding antimicrobial resistance, toxins, and adhesion factors among various SCCmec types of MRSA isolated from intensive care units (ICUs). Methods: From April 2016 to March 2017, a total of 200 MRSA species were isolated from various clinical samples of patients hospitalized in ICUs. The Kirby-Bauer disk diffusion method was employed to determine resistance pattern. Conventional polymerase chain reaction (PCR) assay was utilized to demonstrate antimicrobial resistance, toxins, and adhesion genes. Different SCCmec types of MRSA strains were determine by the multiplex PCR assay. Results: Antibiotic susceptibility testing revealed that all the isolates were sensitive to linezolid, teicoplanin, and vancomycin. The frequency of high-level mupirocin-resistant MRSA was 5.5%. The presence of resistance genes ant(4')-Ia, aac(6')-Ie/aph(2"), tetM, msrA, ph(3')-IIIa, ermA, msrB, ermB, ermC, and mupA were detected 73.5%, 60.5%, 57.5%, 37%, 36.5%, 34.5%, 24%, 17%, 15% and 5.5%, respectively. The most prevalent adhesion genes were clfA (93.5%) followed by clfB (90%), fnbA (81.5%), fnbB (77%), can (51%), ebp (46.5%), and bbp (2.5%). The frequency of etb, eta, pvl, and tst genes were 4.5%, 9.5%, 21.5%, and 61.5%, respectively. Inducible macrolide-lincosamidestreptogramin B (inducible MLSB resistance; iMLSB), and constitutive MLSB (cMLSB) phenotypes were observed in 88 (44%) and 26 (13%) isolates. Different SCCmec types comprising type III (56.5%), type IV (25%), type II (11%) and type I (7.5%) were identified among the MRSA strains. Conclusions: Results of the current study showed a high prevalence of resistance to commonly used antibiotics that can be a serious threat to patients hospitalized in ICUs. Also, the current study findings showed that different SCCmec types causing nosocomial infections were associated with specific antimicrobial resistance, adhesion, and toxin gene profiles.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Distribution of Genes Encoding Toxin, Adhesion, and Antibacterial Resistance Among Various SCCmec Types of Methicillin-Resistant Staphylococcus aureus Isolated From Intensive Care Unit, Tehran, Iran

Abiri

Sepahi

Goudarzi

et al. 2017

Jundishapur J Microbiol

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“…in cases of endocarditis), it could be an important primary urinary pathogen and causes ascending urinary tract colonization and infection (1,2). The most important factor contributing to the successful wide distribution of this pathogen is believed to be the remarkable ability to acquire resistance to antimicrobial agents, to the extent that, nowadays, one of the major public health concerns regarding S. aureus infections is the increasing prevalence of methicillin-resistant S. aureus (MRSA) (3)(4)(5). Resistance to methicillin results from the mecA gene, which is located on a mobile genetic element with 21 to 67 kbp of size, called staphylococcal cassette chromosome (SCC) element (6,7).…”

Section: Introductionmentioning

confidence: 99%

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

Goudarzi

Abiri

Nasirian

et al. 2018

Jundishapur J Microbiol

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Background: Urinary tract infection (UTI) is one of the most frequent types of infections in community and hospital settings. Objectives: The aim of the present study was to investigate antimicrobial susceptibility patterns and also to determine spa and SCCmec types of Staphylococcus aureus strains isolated from patients with UTI. Methods: During a 12-month period, 863 urine samples were investigated. In vitro susceptibility of isolates was performed using the Kirby-Bauer disk diffusion method. Moreover, conventional PCR was performed to detect mecA, nucA, spa, and toxin (pvl, tst) genes as well as Multiplex PCR to determine SCCmec types. Results: A total of 90 isolates (10.4%) were analyzed in the present survey. Methicillin resistance was observed in 61.1% of the isolates. In total, 25 isolates (27.8%) were positive for the pvl-encoding gene and 40 (44.4%) for tst-1 encoding gene. Based on a multiplex PCR assay, the 4 different SCCmec types were detected as type III (38.9%), followed by type II (31.1%), type IV (28.9%), and type I (1.1%). PantonValentine Leukocidin (PVL) positive isolates belonged to SCCmec type IV (14.4%) and II (13.3%). The 90 S. aureus isolates were classified to 10 spa types t037 (23.3%), t924 (15.6%), t383 (15.6%), t426 (12.2%), t044 (12.2%), t790 (6.7%), t084 (4.4%), t021 (4.4%), t7580 (4.4%), and t064 (1.2%). The spa types t044, t790, and t084 were obtained from methicillin-resistant S. aureus (MRSA) strains and spa types t924 and t7580 from methicillin-sensitive S. aureus strains. Conclusions: To the best of the author's knowledge, this is the first study on spa types t426 and t021 in Iran. Since the majority of strains isolated from patients with UTI were MRSA, detection of MRSA clones and their characteristics is necessary.

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Emergence of community-acquired methicillin-resistant Staphylococcus aureus EMRSA-15 clone as the predominant cause of diabetic foot ulcer infections in Portugal

Silva

Almeida

Carvalho

et al. 2019

Eur J Clin Microbiol Infect Dis

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Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis

Cited by 77 publications

References 42 publications

Distribution of Genes Encoding Toxin, Adhesion, and Antibacterial Resistance Among Various SCCmec Types of Methicillin-Resistant Staphylococcus aureus Isolated From Intensive Care Unit, Tehran, Iran

Distribution of Genes Encoding Toxin, Adhesion, and Antibacterial Resistance Among Various SCCmec Types of Methicillin-Resistant Staphylococcus aureus Isolated From Intensive Care Unit, Tehran, Iran

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

Emergence of community-acquired methicillin-resistant Staphylococcus aureus EMRSA-15 clone as the predominant cause of diabetic foot ulcer infections in Portugal

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