Molecular characteristics of Mycobacterium tuberculosis in the “closed” Russian town with limited population migration

Umpeleva, Tatiana; Belousova, Kseniya; Golubeva, Ludmila; Boteva, Tatiana; Morozova, Irina A.; Vyazovaya, Anna; Mokrousov, Igor; Eremeeva, Natalya; Vakhrusheva, Diana

doi:10.1016/j.meegid.2020.104174

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…In GC2, 10/21 strains were isolated from prison inmates, and in GC3, 8/17 of the clustered strains came from prison inmates or individuals with a recent history of incarceration, along with a prison worker. Thus, GC3 represents an important active transmission chain involving the inmate population and the community [6] , similar to that found in other regions in Brazil [39] and worldwide [40] .…”

Section: Discussionsupporting

confidence: 77%

Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

Salvato¹,

Reis²,

Schiefelbein³

et al. 2021

International Journal of Antimicrobial Agents

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Section: Discussionsupporting

confidence: 77%

Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

Salvato¹,

Reis²,

Schiefelbein³

et al. 2021

International Journal of Antimicrobial Agents

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“…Помимо диагностики и лечения, понимание путей передачи возбудителя и ее предотвращения является важной составляющей стратегий борьбы с туберкулезом, наиболее полную картину можно получить только при объединении данных классических эпидемиологических и молекулярно-генетических исследований [33][34][35][36]. В России такой подход пока отсутствует, а единичные исследования, проведенные в разных регионах, с использованием разных подходов и технологий генотипирования (MIRU-VNTR -Mycobacterial interspersed repetitive units -variable number of tandem repeats, сполиготипирования, полногеномного секвенирования и их сочетаний), с отсутствием полных дан-ных об источниках образцов, не позволяют эффективно управлять эпидемическим процессом [37][38][39][40]. Учитывая огромную территорию РФ, создание сети биобанков, работающих по единым стандартам, должно стать основой для проведения научных исследований во фтизиатрии, а также молекулярно-эпидемиологического мониторинга туберкулеза и сочетанных с ним инфекций [41].…”

Section: биобанкирование во фтизиатрииunclassified

Creation of a collection of different biological sample types from elderly patients to study the relationship of clinical, systemic, tissue and cellular biomarkers of accumulation of senescent cells during aging

Sorokina

Orlova

Grigorieva

et al. 2022

Cardiovasc Ther Prev

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With aging, tissue homeostasis and their effective recovery after damage is violated. It has been shown that this may be due to the excessive accumulation of senescent (SC) cells in various tissues, which leads to the activation of chronic sterile inflammation, tissue dysfunction and, as a result, to the development of age-related diseases. To assess the contribution of SC cells to human body aging and pathogenesis of such diseases, relevant biomarkers are studied. For successful translation into clinical practice of approaches aimed at regulating the SC cell content in various tissues, it is necessary to study the relationship between the established clinical biomarkers of aging and age-related diseases, systemic aging parameters, and SC biomarkers at the tissue and cellular levels.Aim. To develop and describe action algorithms for creating a biobank of samples obtained from patients aged >65 years in order to study biomarkers of SC cell accumulation.Material and methods. To collect samples, an interaction system was built between several research, clinical and infrastructure departments of a multidisciplinary medical center. At the stage of preanalytical training, regulatory legal acts were developed, including informed consent for patients, as well as protocols for each stage of the study.Results. A roadmap was formed with action algorithms for all participants in the study, as well as with a convenient and accessible system of annotations and storage of biological samples. To date, the collection includes biological samples of 7 different types (peripheral blood serum, formalin-fixed tissue samples and formalin fixed paraffin embedded tissue specimens, samples of different cells isolated from peripheral blood, skin and adipose tissue, samples of deoxyribonucleic and ribonucleic acids, cell secretome conditioned media) obtained from 82 patients. We accumulated relevant anamnestic, clinical and laboratory data, as well as the results of experimental studies to assess the SC cell biomarkers. Using the collection, the relationship between clinical, tissue and cellular biomarkers of SC cell accumulation was studied.Conclusion. The creation of a collection of biological samples at the molecular, cellular, tissue and organism levels from one patient provides great opportunities for research in the field of personalized medicine and the study of age-related disease pathogenesis.

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“…One of the first articles on the Beijing B0/W148 strain highlighted its circulation in different prisons in Siberia (Portaels, Rigouts, & Bastian, 1999). A recent study in the Russian city of Novouralsk concluded that a high proportion of the former prisoners among TB patients was one of the factors that contributed to an exceptionally high prevalence of the MDR Beijing B0/W148-cluster (Umpeleva et al, 2020). Indeed, the TB control in the penitentiary system in Estonia has been much improved and the number of TB cases diagnosed in prisons decreased from 56 in 1996 to 2 in 2019 (https://statistika.tai.ee/Resources/Info/TSTUA_ en.html.).…”

Section: Tuberculosis Genotypes and Risk Factorsmentioning

confidence: 99%

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

Mokrousov

Vyazovaya

Levina

et al. 2020

Transbounding Emerging Dis

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Different and contrasting trends related to human migration and the implementation of health control programmes influence the spread of drug-resistant tuberculosis (TB). We analysed the Mycobacterium tuberculosis population structure in Estonia, a high-priority EU country for TB control, to detect the dynamic changes and underlying factors. The study collection included 278 M. tuberculosis isolates recovered in 1999 and 2014-2015. The isolates were subjected to drug susceptibility testing, genotyping and analysis of sublineage/cluster-specific markers and drug resistance mutations. The Beijing genotype was the most prevalent, and its rate increased from 28.6% in 1999 to 38.5% in 2015 (p = .09). The non-Beijing strains represented Euro-American lineage (Latin American Mediterranean [LAM], Ural, Haarlem, T, X genotypes) and Indo-Oceanic lineage (one EAI-IND isolate). The proportion of isolates resistant to two or more drugs increased from 22.4% to 29.1% (p = .1). The pre-XDR/ XDR isolates were identified only within the Beijing genotype. In contrast, the drug resistance rate decreased in the LAM genotype from 42.1% to 11.8% (p = .05). The Beijing B0/W148-cluster ('successful Russian strain') included only MDR, pre-XDR or XDR isolates. All B0/W148-cluster isolates were resistant to two or more drugs compared to 28% of the Beijing 94-32-cluster (p = .0002). The Beijing genotype was not identified in the isolates from patients born in Estonia before 1940 compared to its 35.2% rate among other patients. In summary, the circulation of the highly drugresistant isolates of the Beijing B0/W148 subtype, the increased prevalence of the Beijing genotype among HIV-coinfected patients and the increased number of patients with alcohol abuse (47.5%) present major challenges of the current TB control in Estonia. The Beijing genotype was likely brought to Estonia after 1945 due to the massive human influx from the Soviet Union. In contrast, the main genotypes of the Euro-American lineage were likely endemic in Estonia during all 20th century.

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Molecular characteristics of Mycobacterium tuberculosis in the “closed” Russian town with limited population migration

Cited by 10 publications

References 18 publications

Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

Genomic-based surveillance reveals high ongoing transmission of multi-drug-resistant Mycobacterium tuberculosis in Southern Brazil

Creation of a collection of different biological sample types from elderly patients to study the relationship of clinical, systemic, tissue and cellular biomarkers of accumulation of senescent cells during aging

Spatiotemporal dynamics of drug‐resistant Mycobacterium tuberculosis : Contrasting trends and implications for tuberculosis control in EU high‐priority country

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