Molecular Characteristics of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Poultry Farms in China

Lei, Changwei; Zhang, Anyun; Liu, Bihui; Guan, Zhongbin; Xu, Chongxin; Xia, Qingqing; Cheng, Han; Zhang, Dongdong

doi:10.1128/aac.03992-14

Cited by 27 publications

(17 citation statements)

References 19 publications

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“…The four P. mirabilis strains (16.7%) from chicken harbored a SGI1, whereas none of the isolates from the clinical specimens contained SGI1. Surprisingly, the isolation rate (16.7%) of SGI1-bearing P. mirabilis strains confirmed in our study was much higher than that of SGI1-bearing P. mirabilis strains from chicken (9.4%) or swine (4.9%) in China [16,19]. In contrast, our isolation rate from chicken was similar to that (15.6%) of SGI1-bearing P. mirabilis strains from dogs in France, where P. mirabilis isolates carrying SGI1 was first reported and more research of SGI1 continued [14].…”

Section: Discussioncontrasting

confidence: 57%

Molecular Characterization of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Chungcheong Province, Korea

Sung

Kim

Kwon

et al. 2017

Journal of Microbiology and Biotechnology

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The emergence and dissemination of genomic island 1 (SGI1) are strongly associated with the occurrence of multidrug-resistant (MDR) enterobacteria in humans and animals. Diverse SGI1s have been reported among and in several countries. We aimed to characterize SGI1 in isolates from humans and chickens in Chungcheong Province, Korea. A total of 44 isolates were recovered from humans ( = 20) and chickens ( = 24). Antimicrobial susceptibility was determined by disk diffusion assay. To detect and characterize SGI1s, PCR amplification and PCR mapping experiments were performed. Repetitive extragenic palindromic-PCR (REP-PCR) was performed to assess the clonality of the isolates. The four strains (16.7%) from chicken harbored a SGI1, whereas none of the isolates from clinical specimens contained SGI1. The SGI1s detected in our study were all confirmed as SGI1-ABB harboring aminoglycoside-resistant genes (CA5 and A7). In isolates, the presence of SGI1-ABB was significantly correlated with high resistance rates of the isolates to antimicrobial agents, such as gentamicin, streptomycin, and spectinomycin. Moreover, the four SGI1-bearing isolates showed the same REP-PCR patterns and that suggested both horizontal and clonal spread of the isolates. This study is the first attempt to determine SGI1s in isolates in Korea. We confirmed that isolates carrying SGI1-ABB were distributed at poultry farms in Korea. The present study emphasizes the need for continuous monitoring of SGI1s to prevent spreading of the MDR genomic islands among isolates from humans and animals.

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Section: Discussioncontrasting

confidence: 57%

Molecular Characterization of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Chungcheong Province, Korea

Sung

Kim

Kwon

et al. 2017

Journal of Microbiology and Biotechnology

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“…P. mirabilis isolates of animal origin were also shown to carry SGI1 or PGI1. Indeed, SGI1-positive P. mirabilis isolates were reported in poultry and swine farms in China (Lei et al, 2014, 2015). In France, we recently described the very first cases of SGI1 (including the VEB-6-producing SGI1-V variant) or PGI1-positive P. mirabilis in dogs (Schultz et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Detection of SGI1/PGI1 Elements and Resistance to Extended-Spectrum Cephalosporins in Proteae of Animal Origin in France

et al. 2017

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Proteae, and especially Proteus mirabilis, are often the cause of urinary tract infections (UTIs) in humans. They were reported as carriers of extended-spectrum β-lactamase (ESBL) genes, and recently of carbapenemases, mostly carried by the Salmonella genomic island 1 (SGI1) and Proteus genomic island 1 (PGI1). Proteae have also lately become an increasing cause of UTIs in companion animals, but antimicrobial susceptibility data in animals are still scarce. Here, we report the characterization of 468 clinical epidemiologically unrelated Proteae strains from animals collected between 2013 and 2015 in France. Seventeen P. mirabilis strains (3.6%) were positive for SGI1/PGI1 and 18 Proteae (3.8%) were resistant to extended-spectrum cephalosporins (ESC). The 28 isolates carrying SGI1/PGI1 and/or ESC-resistance genes were isolated from cats, dogs, and horses. ESBL genes were detected in six genetically related P. mirabilis harboring blaV EB-6 on the SGI1-V variant, but also independently of the SGI1-V, in 3 P. mirabilis strains (blaVEB-6 and blaCTX-M-15) and 1 Providencia rettgeri strain (blaCTX-M-1). The AmpC resistance genes blaCMY -2 and/or blaDHA-16 were detected in 9 P. mirabilis strains. One strain presented both an ESBL and AmpC gene. Interestingly, the majority of the ESBL/AmpC resistance genes were located on the chromosome. In conclusion, multiple ESC-resistance genetic determinants are circulating in French animals, even though SGI1-V-carrying P. mirabilis seems to be mainly responsible for the spread of the ESBL gene blaVEB-6 in dogs and horses. These results are of public health relevance and show that companion animals in close contact with humans should be regarded as a potential reservoir of ESC-resistant bacteria as well as a reservoir of ESC-resistance genes that could further disseminate to human pathogens.

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“…The results indicated that PsTAN3 carried the entire SGI1 sequence. Additionally, all the SGI1-W-positive strains reported globally were characterized by the absence of any deletion or insertion in the SGI1-W backbone genes (6,7,9). The absence of any deletion in ORF S005 (traN) in SGI1-W of PsTAN3 and the presence of two intact ORFs (S006 and S007) coding for homologs of FlhDC SGI1 will result in increasing SGI1 maintenance, transfer, and/or spreading.…”

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confidence: 99%

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Soliman

Nariya

Shimamoto

2019

Antimicrob Agents Chemother

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M ost of the variants of Salmonella genomic island 1 (SGI1) are multidrug resistance (MDR) DNA elements that can be transferred by horizontal gene transfer (HGT) (1). SGI1 was initially described in Salmonella enterica serovar Typhimurium DT104 (1). It consists of a 27.4-kb backbone (28 open reading frames [ORFs]) with an MDR region consisting of a 15-kb complex class 1 integron of the family In104 (1). The SGI1 variant SGI1-L was detected for the first time in Proteus mirabilis and Morganella morganii strains from Palestine in 2006 and from France in 2017, respectively (2, 3). The 3= end of the chromosomal trmE gene is the SGI1-specific integration site in the SGI1-carrying strains (3, 4). Providencia stuartii is Gram-negative bacterium of the family Morganellaceae commonly associated with urinary tract infections or indwelling urinary catheters in hospitalized and nursing home patients (5). It has intrinsic resistance to polymyxin, tigecycline, aminopenicillin, and first-generation cephalosporins (5). Here, we report the first identification (to our knowledge) of SGI1 in a multidrugresistant clinical isolate of P. stuartii. The strain, designed PsTAN3, was isolated from a wound pus swab from a male patient in a teaching hospital in Tanta City, Egypt, in 2014. PsTAN3 was resistant to aztreonam (MIC, Ն512 g/ml), ciprofloxacin (MIC, Ն16 g/ml), chloramphenicol (MIC, Ն512 g/ml), colistin (MIC, Ն128 g/ml), gentamicin (MIC, Ն128 g/ml), tetracycline (MIC, Ն128 g/ml), streptomycin (MIC, Ն512 g/ml), cefoxitin (MIC, Ն256 g/ml), cefoperazone (MIC, Ն512 g/ml), trimethoprim (MIC, Ն512 g/ml), cefotaxime (MIC, Ն512 g/ml), meropenem (MIC, Ն16 g/ml), and nalidixic acid (MIC, Ն512 g/ml) but sensitive to doripenem (MIC, Յ1 g/ml). PsTAN3 was negative by the modified carbapenem inactivation method (mCIM), indicating the absence of carbapenemase production. PCR and DNA sequencing were used to map the entire SGI1 (for a list of PCR primers, see Table S1 in the supplemental material) and identified variant SGI1-W (6, 7). The multidrug resistance region was 6.54 kb with the genetic arrangement intI1-aadA2-lnuF-qacE⌬1-sul1-orf5-orf6-IS6100 (PsTAN3 class 1 integron GenBank accession no. LC370342) (Fig. 1). SGI1-W was integrated at the 3= end of the trmE gene (Fig. 1). The strain also carried bla TEM-1 , bla DHA-1 , qnrD, and floR genes conferring resistance to third-generation cephalosporins, ciprofloxacin, florfenicol, and chloramphenicol, respectively, and the class 1 integrons aadA2 and lnuF. The MDR region of SGI1 in PsTAN3 was 100% identical to that of SGI1-PmC105 reported in a P. mirabilis isolate from a poultry farm in China (7). Furthermore, the circular form was detected in PsTAN3 after two steps of DNA amplification using primers SGI1circ1 and SGI1circ2 with the expected target size (364 bp) (GenBank accession no. LC370343) (see Fig. S1A in the supplemental material). The sequence of the SGI1-W attP site in PsTAN3 (5=-TTCTGTATCGGGAAGTAA-3=) differs in 1 bp (C¡T) from that of S. enterica DT104 and P. mirabilis (5=-TTCTGTATTGGGAAG...

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Molecular Characteristics of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Poultry Farms in China

Cited by 27 publications

References 19 publications

Molecular Characterization of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Chungcheong Province, Korea

Molecular Characterization of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Chungcheong Province, Korea

Detection of SGI1/PGI1 Elements and Resistance to Extended-Spectrum Cephalosporins in Proteae of Animal Origin in France

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

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