Molecular characteristics of serotype 3 Streptococcus pneumoniae isolates among community-acquired pneumonia patients in Japan

Isozumi, Rie; Ito, Yutaka; Ishida, Tadashi; Hirai, Toyohiro; Ito, Isao; Maniwa, Ko; Hayashi, Michio; Kagioka, Hitoshi; Hirabayashi, Masataka; Onaru, Koichi; Tomioka, Haruaki; Tomii, Keisuke; Gohma, Iwao; Osawa, Makoto; Imai, Seiichiro; Takakura, Shunji; Iinuma, Yoshitsugu; Chin, Kazuo; Ichiyama, Satoshi; Mishima, Michiaki

doi:10.1007/s10156-008-0600-9

Cited by 13 publications

(18 citation statements)

References 14 publications

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“…In the present study we provided the first evidence in Japan of serotype variation of nasopharyngeal S. pneumoniae and H. influenzae isolates from flu + pediatric patients who had just started to develop a fever. For S. pneumoniae, major serotypes were 3, 6, 19, and 23, in agreement with the previous observation that major serotypes are 3, 6, 14, 19, and 23 for S. pneumoniae-associated pneumonia in children and adults, 1,23 and that major serotypes are 1, 6, 19 and 23 for S. pneumoniae-associated meningitis in children. 1,6 In contrast, serotype 11, which is a minor type in invasive infection cases, 1,6 was a major type.…”

Section: Discussionsupporting

confidence: 91%

Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza

Takano

Ozaki

Nitahara

et al. 2009

Pediatrics International

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Section: Discussionsupporting

confidence: 91%

Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza

Takano

Ozaki

Nitahara

et al. 2009

Pediatrics International

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Section: Introductionmentioning

confidence: 99%

“…This lineage is geographically highly widespread, having been found across Europe [21], Japan [22] and North and South America [21], [23]–[25]. Although it is not associated with penicillin resistance, macrolide resistant representatives of the genotype have been identified [22]. Since 2001, this lineage has been observed to increase in prevalence among invasive disease isolates from the USA following the introduction of the heptavalent conjugate vaccine, which does not protect against serotype 3 pneumococci [23].…”

Section: Introductionmentioning

confidence: 99%

Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection

et al. 2013

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Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis of a complete reference genome and 81 draft sequences from clonal complex 180, the predominant serotype 3 clone in much of the world, found most sampled isolates belonged to a clade affected by few diversifying recombinations. However, other isolates indicate significant genetic variation has accumulated over the clonal complex's entire history. Two closely related genomes, one from the blood and another from the cerebrospinal fluid, were obtained from a patient with meningitis. The pair differed in their behaviour in a mouse model of disease and in their susceptibility to antimicrobials, with at least some of these changes attributable to a mutation that up-regulated the patAB efflux pump. This indicates clinically important phenotypic variation can accumulate rapidly through small alterations to the genotype.

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“…Serotype 3 is a less frequent cause of IPD, but nevertheless, cases of type 3 IPD have a high fatality rate (19,22,60). In Europe, Japan, and North America, sequence type 180 (ST180) is the major clone within serotype 3 (7,28,42,60), whereas in South Africa and Ghana, ST458 is more dominant (35,46). The second serotype examined, 11A, is a common nasopharyngeal isolate in Aboriginal children but is rarely isolated from the middle ear (34).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we have analyzed isolates from remote communities using a variety of techniques, including Western blot analysis, a hemolysis assay, a capsular polysaccharide assay, MLST, and murine virulence and colonization models. Although the isolates were acquired from diverse locations, the serotype 3 isolates were found to be from the ST458 clonal cluster, which is unrelated to the ST180 clone that normally dominates in developed countries (7,28,42,60). Interestingly, this ST has also been seen to dominate over ST180 in South Africa and Ghana, which are also socioeconomically disadvantaged (35,46).…”

Section: Downloaded Frommentioning

confidence: 99%

Contribution of Serotype and Genetic Background to Virulence of Serotype 3 and Serogroup 11 Pneumococcal Isolates

et al. 2011

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The capsular serotype has long been associated with the virulence of Streptococcus pneumoniae. Here we present an in-depth study of phenotypic and genetic differences between serotype 3 and serogroup 11 S. pneumoniae clinical isolates from both the general and indigenous populations of Australia. Both serotypes/ groups included clonally unrelated strains with differences in well-known polymorphic virulence genes, such as nanA and pspA, as demonstrated by multilocus sequence typing and Western blot analysis. Nonetheless, the serotype 3 strains were consistently and significantly more virulent in mice than the serogroup 11 strains. Despite extensive genomic analysis, noncapsular genes common to one serotype/group but not the other were not identified. Nevertheless, following the conversion of a serotype 11A isolate to serotype 3 and subsequent analysis in an intranasal infection model, it was evident that both capsular and noncapsular factors determine the virulence phenotype in mice. However, it appears that these noncapsular factors vary from strain to strain.Streptococcus pneumoniae (the pneumococcus) is a formidable pathogen, being responsible for a broad spectrum of diseases, including pneumonia, meningitis, bacteremia, and otitis media (OM), and it accounts for more deaths worldwide than any other single pathogen (48). In Australia, the overall rate of invasive pneumococcal disease (IPD) in the indigenous population is over 4 times the rate seen in nonindigenous Australians (54). Furthermore, chronic suppurative OM, which is normally rare in developed countries, is present in remote Aboriginal communities at 10 times the rate defined as a major public health concern by the World Health Organization (9). Not only do children living in these communities suffer the highest rates of tympanic membrane perforation in the world, but 80% are estimated to suffer from some degree of OMrelated hearing impairment (45).Significant research on the pneumococcus has already been undertaken, but there are still many unanswered questions due to the complex nature of its pathogenicity. The polysaccharide capsule that surrounds the pneumococcus and determines the serotype, of which there are more than 90, is the most wellestablished virulence factor. Certain serotypes have been shown to have a higher association with disease or carriage than others (7,19,20,60). In addition, a number of conserved virulence proteins, including neuraminidase A (NanA), pneumolysin (Ply), autolysin (LytA), and pneumococcal surface proteins A and C (PspA and PspC), have been widely characterized and shown to be critical in pathogenesis (29, 50). However, these are only a subset of potential virulence factors. Like other pathogens, such as Haemophilus influenzae, another common cause of invasive disease and OM, S. pneumoniae has a pangenome (23, 24). Core genes, which are conserved across pneumococcal strains, represent only 70 to 80% of the genome, and a gene pool of over 5,000 orthologous clusters is estimated to be available to this naturally tran...

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Molecular characteristics of serotype 3 Streptococcus pneumoniae isolates among community-acquired pneumonia patients in Japan

Cited by 13 publications

References 14 publications

Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza

Streptococcus pneumoniae and Haemophilus influenzae at the initial stage of influenza

Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection

Contribution of Serotype and Genetic Background to Virulence of Serotype 3 and Serogroup 11 Pneumococcal Isolates

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