Molecular Characteristics of Staphylococcus aureus From Food Samples and Food Poisoning Outbreaks in Shijiazhuang, China

Lv, Guoping; Jiang, Ruiping; Zhang, Han; Wang, Lei; Li, Lijie; Gao, Weili; Zhou, Hong; Pei, Yantao; Xiu-ping, Wei; Dong, Huijuan; Liyun, Qin

doi:10.3389/fmicb.2021.652276

Cited by 34 publications

(17 citation statements)

References 32 publications

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“…However, our study highlighted the presence of other CCs in European countries, for example, the high prevalence of CC398 in the Netherlands, followed by Germany, Italy, and Denmark, remarks the emerging spread of this clone in these areas. Furthermore, CC1 and CC59 are most prevalent in China, as has been found in previous studies [ 21 , 22 , 23 ], while CC8, CC45, and CC121 were the most abundant in Singapore, Taiwan, and Thailand, respectively. Other studies also described differences among Asian countries, for example, Turner et al (2019) [ 24 ] identified CC8 as a common CC in Korea, CC30 in Japan, and CC59 in Taiwan, while Song et al (2011) [ 25 ] described CC8 as widespread throughout Asian Countries and CC59 mostly isolated in Taiwan [ 24 , 25 ].…”

Section: Discussionsupporting

confidence: 73%

Antimicrobial Resistance Genes Analysis of Publicly Available Staphylococcus aureus Genomes

et al. 2022

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Staphylococcus aureus is a pathogen that can cause severe illness and express resistance to multiple antimicrobial agents. It is part of the ESKAPE organisms and it has been included by the Centers for Disease Control and Prevention (CDC) of USA in the list of serious threats to humans. Many antimicrobial mechanisms have been identified, and, in particular, antimicrobial resistance genes (ARGs) can be determined by whole genome sequencing. Mobile genetic elements (MGEs) can determine the spread of these ARGs between strains and species and can be identified with bioinformatic analyses. The scope of this work was to analyse publicly available genomes of S. aureus to characterise the occurrence of ARGs present in chromosomes and plasmids in relation to their geographical distribution, isolation sources, clonal complexes, and changes over time. The results showed that from a total of 29,679 S. aureus genomes, 24,765 chromosomes containing 73 different ARGs, and 21,006 plasmidic contigs containing 47 different ARGs were identified. The most abundant ARG in chromosomes was mecA (84%), while blaZ was the most abundant in plasmidic contigs (30%), although it was also abundant in chromosomes (42%). A total of 13 clonal complexes were assigned and differences in ARGs and CC distribution were highlighted among continents. Temporal changes during the past 20 years (from 2001 to 2020) showed that, in plasmids, MRSA and macrolide resistance occurrence decreased, while the occurrence of ARGs associated with aminoglycosides resistance increased. Despite the lack of metadata information in around half of the genomes analysed, the results obtained enable an in-depth analysis of the distribution of ARGs and MGEs throughout different categories to be undertaken through the design and implementation of a relatively simple pipeline, which can be also applied in future works with other pathogens, for surveillance and screening purposes.

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Section: Discussionsupporting

confidence: 73%

Antimicrobial Resistance Genes Analysis of Publicly Available Staphylococcus aureus Genomes

et al. 2022

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“…S. aureus produces a wide variety of heat-stable staphylococcal enterotoxins (SE) in foods responsible for staphylococcal food poisoning (SFP) in the human population. SFP is characterized by gastroenteritis and has been recognized as one of the main culprits of food poisoning epidemics worldwide [ 39 ]. Methicillin-resistant strains of S. aureus (MRSA) have recently been classified as high priority bacteria with the potential to cause devastating mortality globally if adequate treatment options are not developed [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Knowledge, Hygienic Practices, and Toxi-Infectious Risks Associated with Ready-to-Eat Gbeli: A Particular Chip Derived from Cassava (Manihot esculenta Crantz) Tuber Vended in Streets of Abomey-Calavi Municipality, Benin

Noumavo

Ohin

Fadipe

et al. 2022

BioMed Research International

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The lack of regulations relating to street food remains a real problem in most developing countries. To remedy this, it is essential to have reliable data on the actors and the conditions of streed food activity. The present study is aimed at (i) establishing the sociodemographic profile of producers and vendors of Gbeli in the municipality of Abomey-Calavi, (ii) developing the technical production diagram and assessing the adoption level of good hygienic practices during the production and sale of Gbeli, and finally, (iv) evaluating the microbiological contamination risks associated with Gbeli consumption. Direct observations followed by a semistructured survey on 92 Gbeli vendors were carried out in Abomey-Calavi municipality. A Gbeli sample was collected from each vendor for microbiological analyses according to standard microbiology analytical techniques. Forty vendors were assisted during Gbeli production. The results showed that the production and sale of Gbeli are activities carried out solely by women (100%), mostly adults (97.82%) and unschooled (58.69%). This trade generates income allowing the vendors to contribute to their household expenses and to save. No major difference was noted in the production process of Gbeli contrary to the sale modes (stationary, itinerant, and mixed). Production process, packaging form, and sale condition of Gbeli present enormous hygienic practice deficiencies. Some corrective measures were thus suggested. About 56% of Gbeli samples analyzed were microbiologically not up to standard. The prevalence and abundance of mycotoxinogenic molds, thermotolerant coliforms, and coagulase-positive Staphylococci in these chips are very worrying. The consumption of Gbeli therefore exposes its consumers to a permanent risk of food poisoning. Training of Gbeli vendors on good hygiene and production practices is indispensable.

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“…However, other less well-known clones, such as ST6 and ST398, have also been reported. ST6 has been implicated in infectious transmission in communities and hospitals and ST15 in cystic fibrosis patients in China, Europe and the Czech Republic [ 50 , 51 ]. Concerning ST398, which has rarely been described in Algeria [ 44 ], it was initially associated with livestock and subsequently detected in workers in close contact with livestock [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria

et al. 2022

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Staphylococcus aureus causes a wide range of life-threatening infections. In this study, we determined its prevalence in the hospital environment and investigated nasal carriage among healthcare workers and patients admitted to a hospital in western Algeria. A total of 550 specimens were collected. An antibiogram was performed and the genes encoding resistance to methicillin, inducible clindamycin and toxins were sought among the 92 S. aureus isolates. The spread of clones with a methicillin- and/or clindamycin-resistance phenotype between these ecosystems was studied using genomic analysis. A prevalence of 27%, 30% and 13% of S. aureus (including 2.7%, 5% and 1.25% of MRSA) in patients, healthcare workers and the hospital environment were observed, respectively. The presence of the mecA, erm, pvl and tsst-1 genes was detected in 10.9%, 17.4%, 7.6% and 18.5% of samples, respectively. Sequencing allowed us to identify seven sequence types, including three MRSA-IV-ST6, two MRSA-IV-ST80-PVL+, two MRSA-IV-ST22-TSST-1, two MRSA-V-ST5, and one MRSA-IV-ST398, as well as many virulence genes. Here, we reported that both the hospital environment and nasal carriage may be reservoirs contributing to the spread of the same pathogenic clone persisting over time. The circulation of different pathogenic clones of MRSA, MSSA, and iMLSB, as well as the emergence of at-risk ST398 clones should be monitored.

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Molecular Characteristics of Staphylococcus aureus From Food Samples and Food Poisoning Outbreaks in Shijiazhuang, China

Cited by 34 publications

References 32 publications

Antimicrobial Resistance Genes Analysis of Publicly Available Staphylococcus aureus Genomes

Antimicrobial Resistance Genes Analysis of Publicly Available Staphylococcus aureus Genomes

Knowledge, Hygienic Practices, and Toxi-Infectious Risks Associated with Ready-to-Eat Gbeli: A Particular Chip Derived from Cassava (Manihot esculenta Crantz) Tuber Vended in Streets of Abomey-Calavi Municipality, Benin

Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria

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