Molecular characterization and antibiotic susceptibility of<i>Vibrio cholerae</i>non-O1

Dalsgaard, Anders; Serichantalergs, Oralak; Pitarangsi, Chittima; Echeverria, Peter

doi:10.1017/s0950268800051906

Cited by 26 publications

(31 citation statements)

References 34 publications

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“…h Non-O1, non-O139 strains isolated from patients in Thailand (18). i O1 strains isolated from patients in Samutsakorn, Thailand (17).…”

Section: Resultsmentioning

confidence: 99%

“…A total of 176 V. cholerae strains, mainly isolated in Thailand, were tested for the presence and contents of class 1 integrons. The strain collection was selected from a large number of V. cholerae strains studied previously for various phenotypic and genotypic characteristics (5,14,(16)(17)(18)(19). The majority of strains were sulfonamide resistant, but strains showing intermediate resistance or susceptibility to sulfonamides were also studied.…”

Section: Methodsmentioning

confidence: 99%

“…Studies of non-O1, non-O139 strains from Thailand showed that strains frequently contained small-size plasmids. However, the plasmids did not appear to encode antibiotic resistance (14,18).…”

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confidence: 99%

“…The importance of non-O1, non-O139 serotypes of V. cholerae as causes of diarrhea are increasingly being recognized (13,14,18,33,41). Although high prevalences of multipleantibiotic-resistant non-O1, non-O139 strains have been reported recently, little is known about the mechanisms of resistance (14,33,41).…”

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confidence: 99%

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Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand

Dalsgaard

Forslund

Serichantalergs

et al. 2000

Antimicrob Agents Chemother

107

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In this study, 176 clinical and environmental Vibrio cholerae strains of different O serotypes isolated inThailand from 1982 to 1995 were selected and studied for the presence of class 1 integrons, a new group of genetic elements which carry antibiotic resistance genes. Using PCR and DNA sequencing, we found that 44 isolates contained class 1 integrons harboring the aadB, aadA2, blaP1, dfrA1, and dfrA15 gene cassettes, which encode resistance to gentamicin, kanamycin, and tobramycin; streptomycin and spectinomycin; ␤-lactams; and trimethoprim, respectively. Each cassette array contained only a single antibiotic resistance gene. Although resistance genes in class 1 integrons were found in strains from the same epidemic, as well as in unrelated non-O1, non-O139 strains isolated from children with diarrhea, they were found to encode only some of the antibiotic resistance expressed by the strains. Serotype O139 strains did not contain class 1 integrons. However, the appearance and disappearance of the O139 serotype in the coastal city Samutsakorn in 1992 and 1993 were associated with the emergence of a distinct V. cholerae O1 strain which contained the aadA2 resistance gene cassette. A 150-kb self-transmissible plasmid found in three O1 strains isolated in 1982 contained the aadB gene cassette. Surprisingly, several strains harbored two integrons containing different cassettes. Thus, class 1 integrons containing various resistance gene cassettes are distributed among different V. cholerae O serotypes of mainly clinical origin in Thailand.

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“…h Non-O1, non-O139 strains isolated from patients in Thailand (18). i O1 strains isolated from patients in Samutsakorn, Thailand (17).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand

Dalsgaard

Forslund

Serichantalergs

et al. 2000

Antimicrob Agents Chemother

107

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“…Studies performed in Tanzania and Bangladesh reported strains of V. cholerae with plasmid-mediated resistance to a wide range of antibiotics (Mhalu et al 1979, Threlfall & Rowe 1982. Dalsgaard et al (1995) reported that of 23 environmental V. cholerae non-O1 strains, 78.3% exhibited resistance to tetracycline and 8.7% were resistant to ampicillin and chloramphenicol. These results differ from the results of the present study.…”

Section: Discussionmentioning

confidence: 99%

Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern

Isaac-Márquez

Lezama-Dávila

Eslava-Campos

et al. 1998

Mem. Inst. Oswaldo Cruz

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Vibrio

Farmer

Janda

Brenner

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Vib' ri.o . L. v. vibrio move rapidly back and forth, vibrate; M.L. masc. n. Vibrio the vibrating, darting organism. Proteobacteria / Gammaproteobacteria / “Vibrionales” / Vibrionaceae / Vibrio Small, straight, slightly curved, curved, or comma‐shaped rods , 0.5–0.8 × 1.4–2.6 µm. Involution forms often occur in old cultures and are formed under adverse growth conditions. Do not form endospores or microcysts. Gram negative. In liquid media, motile by monotrichous or multitrichous polar flagella enclosed in a sheath continuous with the outer membrane of the cell wall. On solid media, some species synthesize numerous lateral flagella with a wavelength shorter than that of the sheathed polar flagellum. Facultative anaerobes capable of both fermentative and respiratory metabolism . Molecular oxygen is a universal electron acceptor. Most do not denitrify or fix molecular nitrogen. All are chemoorganotrophs; most are able to grow in a mineral medium containing D ‐glucose as the sole carbon source and NH + as the sole nitrogen source . A few strains have organic growth factor requirements. Na 4 + stimulates growth of all species and is an absolute requirement for most ; the minimal concentration necessary for optimal growth ranges from 5 to 700 mM (0.029–4.1%). Most species grow well in media containing a seawater base. All ferment D ‐glucose producing acid but rarely gas ; several species produce acetoin and acetyl methyl carbinol ( positive Voges–Proskauer test ). Most ferment and utilize D ‐fructose, maltose, and glycerol; are oxidase positive and reduce nitrate to nitrite . Several grow at 4°C; all grow at 20°C; most grow at 30°C; many grow at 35–37°C. A few species are bioluminescent , as are a few strains of normally nonluminescent species. Primarily aquatic ; species distribution is usually dependent on Na + and nutrient content of the water as well as its temperature. Very common in marine and estuarine environments and on the surfaces and in the intestinal contents of marine animals. Species with a low Na + requirement are also found in freshwater habitats. Twelve species occur in human clinical specimens; 11 of these are apparently pathogenic for humans, causing diarrhea or extraintestinal infections. Several species cause diseases of other vertebrates and invertebrates . The mol % G + C of the DNA is : 38–51. Type species : Vibrio cholerae Pacini 1854, 411.

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Molecular characterization and antibiotic susceptibility ofVibrio choleraenon-O1

Cited by 26 publications

References 34 publications

Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand

Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand

Serotypes of Vibrio cholerae Non-O1 Isolated from Water Supplies for Human Consumption in Campeche, México and their Antibiotic Susceptibility Pattern

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