Molecular characterization and antibiotic susceptibility profiles of Helicobacter pylori isolated from patients with Gastrodeudenal diseases in Jordan

Abu-Qatouseh, Luay; Abu-Sini, Mohammad; Mayyas, Amal; Darwish, Rula M.; Aburjai, Talal; abdoh, Qusai abdoh Qusai; Sabri, Israr

doi:10.3823/794

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Although more boys failed to resolve their infection, which was consistent with data previously reported by Hu et al [59], in our cohort, this was not statistically significant. Unfortunately, our cohort is a retrospective cohort, the cause of non-resolution cannot be judged with confidence, whether due to ineffectiveness of eradication, especially with the high level of drug resistance in our population [66], or due to re-infection in the families with the high prevalence rate in our population [67].…”

Section: Discussionmentioning

confidence: 92%

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Helicobacter pylori gastritis in Jordanian children: persistence versus resolution

Altamimi,

Salameh,

AlNsou

et al. 2024

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Introduction: Helicobacter pylori (H. pylori) is the most common cause of infectious gastritis. Helicobacter pylori is an infection that is typically acquired during childhood.Aim: This study aims to describe children with H. pylori infection and compare the clinicopathological features of children with resolved and persistent infection.Material and methods: This is a retrospective review of all children with biopsy-proven H. pylori infection over a 6-year period. Hospital electronic files, endoscopic database, and histopathology database were reviewed. Patients who underwent follow-up endoscopy were identified, and their data was compared.Results: A total of 176 patients were identified, of whom 100 (56.2%) were females. The average age at presentation was 10.2 years (range: 2.5-17 years). Children older than 10 years were the most affected age group. The most commonly presenting symptom was recurrent abdominal pain (92 (51.69%)), followed by epigastric pain/dyspepsia and vomiting (44 (24.72%) and 18 (10.11%), respectively). The most common macroscopic feature was antral nodularity (76 (42.70%)). The most prevalent microscopic findings were moderate inflammation of moderate chronicity. None of the patients developed gastric atrophy. Forty-four (23.5%) patients had comorbid diseases. On follow-up, upper endoscopy was available for 42 (23.59%) patients. The resolution of H. pylori based on histological examination was observed in only 10 (23.81%) patients. Children whose infections resolved were older.Conclusions: A significant number of children with biopsy-proven H. pylori infection presented with abdominal pain. Female gender, older age, and less severe macroscopic and microscopic findings may be associated with a higher chance of infection resolution.

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Section: Discussionmentioning

confidence: 92%

“…Metronidazole is used only if the patient has a penicillin allergy. A previous study on the antibiotic resistance profile of H. pylori isolates from Jordanian patients showed that the prevalence of metronidazole resistance was very high (92%) while that for clarithromycin was 23% [66].…”

Section: Discussionmentioning

confidence: 93%

Helicobacter pylori gastritis in Jordanian children: persistence versus resolution

Altamimi,

Salameh,

AlNsou

et al. 2024

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“…CLR is a prescription macrolide antibiotic [ 13 ]. It is administered orally to treat certain bacterial infections caused by specific types of bacteria, such as community-acquired pneumonia, throat infections (pharyngitis), acute sinus infections, H-pylori, and others [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Patch Loaded with Clarithromycin Nanovesicles for Transdermal Drug Delivery

Alkilani

Musleh

Hamed

et al. 2023

JFB

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Clarithromycin (CLR), categorized as a Biopharmaceutical Classification System class II drug, has several gastrointestinal tract side effects and an extremely unpalatable bitter taste. The current study aimed to design transdermal patch-embedded CLR niosomes to overcome the aforementioned CLR-related challenges. Various niosomal formulations were successfully fabricated and characterized for their morphology, size, in vitro release, and antimicrobial efficacy. Subsequently, the CLR niosomes were loaded into transdermal patches using the solvent casting method. The polydispersity index of the niosomes ranged from 0.005 to 0.360, indicating the uniformity of the niosomes. The encapsulating efficiency (EE)% varied from 12 to 86%. The optimal Chol: surfactant ratio for drug release was found to be 0.5:1. In addition, the encapsulation of CLR into niosomal nanovesicles did not reduce the antibacterial activity of the CLR. The niosomal patch had a significantly higher permeability coefficient of CLR than the conventional patch. In addition to that, a shear-thinning behavior was observed in the niosomal gels before loading them into a niosomal patch. The flux (Jss) of the niosomal patch was significantly higher than the conventional patch by more than 200 times. In conclusion, niosome-based transdermal patches could be a promising method for the transdermal drug delivery of class II drugs and drugs experiencing GIT side effects.

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In vitro Anti-Helicobacter pylori Activity of Capsaicin

Tayseer¹,

Aburjai²,

Abu-Qatouseh³

et al. 2020

J. Pure Appl. Microbiol.

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Worldwide, peptic ulcer and gastritis considered to be one of the biggest health challenge, Helicobacter pylori is responsible for more than eighty percent of chronic active gastritis where continual infection remains for decennary. However, the success of commercially available drugs for the management of H. pylori has overwhelmed by antibiotic-resistant strains, especially, metronidazole and clarithromycin, therefore, an urgent need arise to search for new options for treatment with enhanced anti-H. pylori activities, while being less toxic to human cells. Naturally occurring plant products, including spices, are one of these strategies that showed activity against H.pylori. Present study aim to test the antibacterial activity of capsaicin and other pure plant-derived compounds against a standard (NCTC 11916) H. pylori strain In vitro and to test for possible synergistic effect when combined with conventional therapy. Capsaicin shows good antibacterial activity on regular antimicrobial sensitivity testing methods (Anti-MSTM) and titration checkerboard assay MIC (0.0625 mg/ml), whereas piperine MIC was (0.125 mg/ ml). While for curcumin no inhibition was found. The strain was found to be resistant to metronidazole with (MIC=250 μg/ml). When combining capsaicin with metronidazole, (FIC) Fractional inhibitory concentration values shown a synergistic effect, While the additive effect was found for capsaicin combination with piperine. Our obtained data indicate that capsaicin possesses promising anti H.pylori bioactivity and synergistic activity when combined with metronidazole but more work is necessary to examine the mechanisms by which these happened. Furthermore, it is necessary to ensure its activity against H.pylori In vivo and clinical settings.

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Molecular characterization and antibiotic susceptibility profiles of Helicobacter pylori isolated from patients with Gastrodeudenal diseases in Jordan

Cited by 4 publications

References 34 publications

Helicobacter pylori gastritis in Jordanian children: persistence versus resolution

Helicobacter pylori gastritis in Jordanian children: persistence versus resolution

Preparation and Characterization of Patch Loaded with Clarithromycin Nanovesicles for Transdermal Drug Delivery

In vitro Anti-Helicobacter pylori Activity of Capsaicin

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