Molecular Characterization and Diagnosis of Macrophomina phaseolina: A Charcoal Rot Fungus

Babu, Bandamaravuri Kishore; Saikia, Ratul; Arora, Dilip K.

doi:10.1007/978-3-642-05042-8_9

Cited by 10 publications

(9 citation statements)

References 49 publications

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“…The study showed variations in the morphological characteristics, including intensity of aerial mycelia, dense of sclerotia formation spreading progress of colony and influence of different solid media on their pathogenicity. Babu et al (2010) reported such differences among the isolates from hosts as sorghum, soybean, chickpea, and corn. M. phaseolina is one of the most pathogens that infect more than 500 plant species throughout the world (Khan, 2007).…”

Section: Discussionmentioning

confidence: 97%

Distinguished Positive Reactions of Macrophomina phaseolina (Tassi) Goid Host Plants, Laboratory Media, and Potting soil

Muhanna

2020

Egyptian Journal of Phytopathology

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Fourteen isolates of Macrophomina phaseolina (Tassi) Goid the causal of charcoal rot, were isolated from different hosts dominated at different locations in Egypt. Macromorphological features of isolates were compared by growing on different agar media at 28±2ºC. Colony appearance, growth rate, aerial mycelia, and production of sclerotia were assessed. Four isolates were scored as fast grow with cod No. M4, M9, M12, M15 and three as slow grow, i.e., M1, M2, M13. The best media for growth were Lima bean Agar (LBA), Czapek's Dox Agar (CZA), Potato Dextrose Agar (PDA), Corn Agar (CA), and potato-sucrose agar (PSA) respectively. The colony characteristic of color was of black dense, light black dense, light grey and dark brown. The best media for sclorotia formation were LBA and PDA media for the isolates M4, M7, M9, M11, M14, M15. Pathogenicity experiments showed that M. phaseolina isolates were able to cause root rot and hypocotyl discoloration of hosts. Three fungal isolates appeared to be less virulent i.e., M2, M3 and M8 whereas two isolates i.e., M11and M15 were scored, highly virulent or drastically virulent to beans, cantaloupe, tomato, cotton and sunflower plants. No relationship between the morphological characteristics and pathogenicity of the isolates. In addition, protein analysis assay of M. phaseolina revealed different numbers of separated bands (1-10) that protein profiling for isolates of M. phaseolina was not related to their virulence, host variety or location. In a greenhouse study, different soil types and bean were used for pathogenicity test of the fungus. Disease severity ranged from 21.3-44.0 %.

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Section: Discussionmentioning

confidence: 97%

Distinguished Positive Reactions of Macrophomina phaseolina (Tassi) Goid Host Plants, Laboratory Media, and Potting soil

Muhanna

2020

Egyptian Journal of Phytopathology

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“…Morphological identification of M. phaseolina is very difficult and often not reliable because of the variation among isolates ( Babu et al, 2010b ; Saleh et al, 2010 ). Biochemical and serological techniques are being employed to identify the fungus but their specificity is limited ( Srivastava and Arora, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

“…Advances in molecular techniques have provided alternative techniques for the reliable identification of fungi. Internal transcribed spacers (ITS) and 18S rRNA have been one of the most conserved genes used to identify the fungus ( Babu et al, 2010b ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Genetic Characterization of Macrophomina phaseolina Strains Causing Pathogenicity on Sunflower and Chickpea

et al. 2017

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Macrophomina phaseolina is the most devastating pathogen which causes charcoal rot and root rot diseases in various economically important crops. Three strains M. phaseolina 1156, M. phaseolina 1160, and M. phaseolina PCMC/F1 were tested for their virulence on sunflower (Helianthus annuus L.) and chickpea (Cicer arietinum L.). The strains showed high virulence on both hosts with a disease score of 2 on chickpea and sunflower. The strains also increased the hydrogen per oxide (H2O2) content by 1.4- to 1.6-fold in root as well as shoot of chickpea and sunflower. A significant increase in antioxidant enzymes was observed in fungal infected plants which indicated prevalence of oxidative stress during pathogen propagation. The M. phaseolina strains also produced hydrolytic enzymes such as lipase, amylase, and protease with solubilization zone of 5–43 mm, 5–45 mm, and 12–35 mm, respectively. The M. phaseolina strains were identified by 18S rRNA and analyzed for genetic diversity by using random amplified polymorphic DNA (RAPD) markers. The findings based on RAPD markers and 18S rRNA sequence analysis clearly indicate genetic variation among the strains collected from different hosts. The genetically diverse strains were found to be pathogenic to sunflower and chickpea.

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“…1F), los cuales se desarrollaron en el tejido del hospedante. Además, de acuerdo con Babu et al 2 , M. phaseolina es una especie muy heterogénea respecto de su morfología, fisiología, ecología y características gené-ricas.…”

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“…Asimismo, la comparación de las secuencias KJ577740 y KJ577741 obtenidas en este estudio mostró un 100 % de similitud con las secuencias KF951786-KF951802 depositadas en la base de datos del GenBank. De acuerdo con Babu et al 2 , las secuencias de ADN obtenidas con los iniciadores específicos MpKFI y MpKRI pueden ser utilizadas para identificar de manera rápida, selectiva y específica el hongo M. phaseolina. De manera similar, Zhang et al 15 y Sánchez et al 12 usaron los iniciadores específicos MpKFI y MpKRI para la identificación y detección de aislados de M. phaseolina en el cultivo de fresa y frijol mungo (Vigna radiata L.), respectivamente.…”

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Caracterización morfológica y molecular de aislados de Macrophomina phaseolina asociados a caña de azúcar en México

Leyva-Mir

Velázquez-Martínez

Tlapal-Bolaños

et al. 2015

Revista Argentina de Microbiología

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Charcoal rot caused by Macrophomina phaseolina is an important disease of sugarcane in Mexico. This study was carried out to characterize isolates of M. phaseolina obtained from sugarcane by the combination of morphological and molecular analyses. The morphological characterization of 10 isolates was performed using scanning electron microscopy and light microscopy. To confirm the morphological identification, rDNA from two representative isolates was extracted, and the internal transcribed spacer (ITS) region was amplified by polymerase chain reaction and sequenced using specific primers MpKF1 and MpKR1. Based on their morphological characteristics, all isolates were identified as M. phaseolina. Moreover, the analysis of two ITS sequences showed 100% similarity with the M. phaseolina sequences deposited in the GenBank. To our knowledge, this is the first study in the world aimed at characterizing isolates of M. phaseolina obtained from sugarcane.

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Molecular Characterization and Diagnosis of Macrophomina phaseolina: A Charcoal Rot Fungus

Cited by 10 publications

References 49 publications

Distinguished Positive Reactions of Macrophomina phaseolina (Tassi) Goid Host Plants, Laboratory Media, and Potting soil

Distinguished Positive Reactions of Macrophomina phaseolina (Tassi) Goid Host Plants, Laboratory Media, and Potting soil

Molecular Identification and Genetic Characterization of Macrophomina phaseolina Strains Causing Pathogenicity on Sunflower and Chickpea

Caracterización morfológica y molecular de aislados de Macrophomina phaseolina asociados a caña de azúcar en México

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