In this study, fourteen genotypes of Brassica (B. juncea) were analyze for correlation co-efficient between oil and its major fatty acid traits and path co-efficient analysis to examine the cause and consequences relationship with direct and indirect components. Correlation coefficient for the major fatty acids revealed that oil content exhibit highly significant with positive correlation and significant for linoleic acid.Moreover, oil content correlated with positively in the direction for palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and erucic acid. Howeverit exhibit in negative direction and non-significant for eicosenoic acid. When dependent characters was oil content and all other residual 7 characters / all fatty acid traits considered as independent contributing characters, linoleic acid (0.180) displayed high order of direct effect on oil content and it is followed by erucic acid (0.154), oleic acid (0.148), Linolenic acid (0.096), palmitic acid (0.095), and stearic acid (0.068). At phenotypic level, linoleic acid (0.115) exhibit high order of direct effect on oil content and it is follow by erucic acid (0.101), oleic acid (0.095), palmitic acid (0.062), Linolenic acid (0.055) and stearic acid (0.045). Residual effect at genotypic level (0.959) and phenotypic (0.983) were high which indicate that most of the characters contributing to the oil content has taken in the present study. Palmitic acid contributed to oil content indirectly via eicosenoic acid; stearic acid via linoleic acid; linoleic acid via stearic acid; Linolenic acid via eicosenoic acid; eicosenoic acid via oleic acid & erucic acid via eicosenoic acid. These characters also been identified as major direct and indirect contributors towards oil contents and fatty acid profile in Indian mustard.