Molecular characterization and epitope-based vaccine predictions for ompA gene associated with biofilm formation in multidrug-resistant strains of A.baumannii

Sogasu, Deepthi; Girija, A. S. Smiline; Gunasekaran, Shoba; Priyadharsini, J. Vijayashree

doi:10.1007/s40203-020-00074-7

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…Similarly, members of the OmpA family are essential OMPs, which have been widely studied in Acinetobacter baumanni [40], Mannheimia haemolytica, E. coli [41], Haemophilus species, and others [42,43]. In general, OmpA-like proteins sense and respond to external stimuli or stressors [44].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Three New Outer Membrane Adhesion Proteins in Fusobacterium necrophorum

Bista,

Pillai,

Narayanan

2023

Microorganisms

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Fusobacterium necrophorum, an anaerobic Gram-negative pathogen, causes necrotic cattle infections, impacting livestock health and the US feedlot industry. Antibiotic administration is the mainstay for treating F. necrophorum infections, although resistance hampers their effectiveness. Vaccination, especially targeting outer membrane proteins (OMPs) due to their antigenic properties and host specificity, offers an alternative to antibiotics. This study identified high-binding-affinity adhesion proteins from F. necrophorum using binding and pull-down assays with bovine adrenal gland endothelial cells (EJG). Four OMP candidates (17.5 kDa/OmpH, 22.7 kDa/OmpA, 66.3 kDa/cell surface protein (CSP), and a previously characterized 43 kDa OMP) were expressed as recombinant proteins and purified. Rabbit polyclonal antibodies to recombinant OMPs were generated, and their ability to inhibit bacterial binding in vitro was assessed. The results show that treatment with individual polyclonal antibodies against 43 kDa significantly inhibited bacterial adhesion, while other antibodies were less potent. However, combinations of two or more antibodies showed a more prominent inhibitory effect on host-cell adhesion. Thus, our findings suggest that the identified OMPs are involved in fusobacterial attachment to host cells and may have the potential to be leveraged in combination for vaccine development. Future in vivo studies are needed to validate their roles and test the feasibility of an OMP-based subunit vaccine against fusobacterial infections.

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Section: Discussionmentioning

confidence: 99%

Characterization of Three New Outer Membrane Adhesion Proteins in Fusobacterium necrophorum

Bista,

Pillai,

Narayanan

2023

Microorganisms

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“…In another study, out of 10 epitopes, 5 epitopes with high score were selected for further analysis of antigenicity. Out of 5 epitopes, 2 epitopes were predicted as probable antigens with the antigenic score of > 0.5 [58]. In another study, out of 10 epitopes, 3 epitopes were selected since it showed positive values [59].…”

Section: Discussionmentioning

confidence: 99%

Epitope Based Vaccine Peptide Predictions for fimH gene of Acinetobacter baumannii

Girija

Ganesh

et al. 2021

JPRI

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Background: Acinetobacter baumannii is an opportunistic bacterial pathogen that is primarily associated with hospital-acquired infections. Recently, there is a dramatic increase in the incidence of multidrug-resistant (MDR) strains, which has significantly raised the profile of this emerging opportunistic pathogen. MDR is often associated with the formation of biofilms and various other virulence factors. Amidst all the genes, fimH gene is our area of interest in this research, because it is an important virulence factor in A. baumannii which encodes the Type 1 fimbriae, that helps bacteria bind to the surface of host cells initiating further infection. Aim: The aim of this study was to assess the epitope based vaccine epitope peptide predictions for the fimH protein of A. baumannii. Materials and Methods: The fimH gene for epitope prediction was selected. Druggability and physico-chemical properties were analysed. Antigenicity was predicted. Epitope mapping of T-cell MHC class 1, Class 1 immunogenicity, conservancy and toxicity analysis was done. T-cell class II epitopes were further mapped together with the immuno-dominant B-cell epitopes. Results: From the selected 20 epitopes, 2 best epitopes (AALVASVCL and YSSGANAFT) were selected after analysing their antigenicity and allergenicity. The epitope YSSGANAFT showed better values in association with HLA alleles - HLA-DP, HLA-DQ, HLA-DR and TLR-2. Conclusion: The finding of the study documents a single immunodominant peptide (sequence) as a promising vaccine candidate to treat infections caused by A. baumannii. However further experimental analysis must be performed to assess the immunological memory and response of the peptide in both in-vitro and in-vivo studies.

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“…Computational based approaches seem to be of higher value in assessing the drug ligand interactions based docking studies. In a previous study, csgA protein of A.baumannii and the anti-biofilm activity of A.indica was assessed and predictions of epitope peptides against the virulent protein of A.baumanni has also been assessed [24,25]. However no earlier studies had documented the inhibitory effect of Ocimum sanctum biocompounds on ptk of A.baumannii.…”

Section: Discussionmentioning

confidence: 99%

A Computational Approach on the Anti-biofilm Effect of Ocimum sanctum Bio-compounds Against ptk of Acinetobacter baumannii

Kamalli

Girija

Ganesh

et al. 2021

JPRI

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Introduction: Acinetobacter baumannii is a gram negative coccobacilli often considered as a nosocomial pathogen and as an opportunistic pathogen in immunocompromised patients. It is considered to be multi-drug resistant and a potent bacteria forming vital biofilms. Ptk which is protein tyrosine kinase is a protein coding gene involved with the synthesis of capsular polysaccharide. Ocimum sanctum is a perennial plant belonging to the Lamiaceae family. Tulsi and holy basil are the common names of this plant. In-silico docking approach method is much more convenient and cost effective to assess the bioactive properties of the natural drugs against any target ligands. Aim: The aim of the study to assess the inhibitory effect of Ocimum sanctum bio-compounds against ptk of Acinetobacter baumannii using a computational approach. Materials and Methods: Retrieval of the structure of ptk was followed by Ligand preparation and optimisation. Further drug likeliness was assessed using Molinspiration parameters, docking simulations and visualisation for the binding energy and hydrogen bonds. Results: Among the bio compounds of O.sanctum, benzofuran is selected as an active inhibitory compound with -11.12 as its binding energy showing a high affinity. Conclusion: The findings of the present study documents benzofuran as the promising candidate to design novel drugs from O.sanctum and to target the ptk of A.baumannii. However further experimental validation must be done to observe its efficacy and safety in the treatment of nosocomial infections caused by A.baumannii.

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Molecular characterization and epitope-based vaccine predictions for ompA gene associated with biofilm formation in multidrug-resistant strains of A.baumannii

Cited by 11 publications

References 33 publications

Characterization of Three New Outer Membrane Adhesion Proteins in Fusobacterium necrophorum

Characterization of Three New Outer Membrane Adhesion Proteins in Fusobacterium necrophorum

Epitope Based Vaccine Peptide Predictions for fimH gene of Acinetobacter baumannii

A Computational Approach on the Anti-biofilm Effect of Ocimum sanctum Bio-compounds Against ptk of Acinetobacter baumannii

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