Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

Ahmadi, Behzad; Masoomi‐Aladizgeh, Farhad; Shariatpanahi, Mehran E.; Azadi, Pejman; Keshavarz-Alizadeh, Mehdi

doi:10.1007/s00299-015-1878-6

Cited by 27 publications

(24 citation statements)

References 46 publications

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“…Furthermore, it was observed in lettuce (Lactuca sativa L.) that the inhibition of endogenous SERK expression by antisense RNA induced a reduction in the formation of in vitro somatic embryonic structures (Santos et al 2009). In this paper, GhSERK2 expression level was highly induced in globular embryos at early phases of SE, and the expression pattern was consistent with those in other species (Che et al 2006;Talapatra et al 2014;Ahmadi et al 2016;Rajesh et al 2016). It was also found that Ser/Thr protein kinase like protein might be involved in cellular communication or signal transduction in SE of cotton (Zeng et al 2006).…”

Section: Discussionsupporting

confidence: 83%

“…SERKs are a subgroup of leucine-rich repeats and receptor-like kinases (LRR-RLKs) (Ma et al 2012a, b) that were first isolated as genes involving in signal transduction in embryonic cell development (Schmidt et al 1997). After that, the SERK homologs were isolated from both monocotyledons (Somleva et al 2000;Baudino et al 2001;Hu et al 2005;Bhumica et al 2008;Cueva et al 2012), and dicotyledons (Hecht et al 2001;Thomas et al 2004;Santos et al 2005;Schellenbaum et al 2008;Sharma et al 2008;Nolan et al 2009;Pérez-Núñez et al 2009;Hedayat et al 2010;Huang et al 2010;Yang et al 2011;Ma et al 2012b;Shi et al 2012Shi et al , 2014Ahmadi et al 2016;Rocha et al 2016). SERKs located in membranes and these homologs were responsible for the cellular signaling and metabolic regulation, which have an important role in the acquisition of embryogenic competence in plant cells (Albrecht et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of GhSERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton

Liu

Zhao

Zeng

et al. 2018

Journal of Integrative Agriculture

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Somatic embryogenesis (SE) is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE (SERK) gene is known to function in SE. A homolog GhSERK2 (Accession number: JF430801) was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid (IBA), the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid (2,4-D) induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus (EC). The level of hormones, including 3-indoleacetic acid (IAA), abscisic acid (ABA), and brassinosteroid (BR), were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Characterization of GhSERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton

Liu

Zhao

Zeng

et al. 2018

Journal of Integrative Agriculture

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“…The hypothesis that SERK1 is a flexible RLK co‐regulator is supported by the fact that it is expressed in several differentiated tissues, including the procambia of vascular bundles of roots, hypocotyls and inflorescence stems (Kwaaitaal and de Vries ) as well as in the primary meristems of the root, the lateral roots and the meristems of the shoot (Nolan et al ). SERK1 might co‐regulate sporogenesis and gametogenesis (Albrecht et al ), the development of the tapetum and the maturation of microspores (Colcombet et al , Ahmadi et al ), brassinosteroid signaling (Karlova et al , Albrecht et al 2008, Gou et al ) and zygotic and somatic embryogenesis (Ahmadi et al ).…”

Section: Introductionmentioning

confidence: 99%

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Pérez-Pascual

Jiménez-Guillen

Villanueva-Alonzo

et al. 2018

Physiologia Plantarum

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Somatic embryogenesis receptor-like kinase 1 (SERK1) is a membrane receptor that might serve as common co-regulator of plant cell differentiation processes by forming heterodimers with specific receptor-like kinases. The Coffea canephora SERK1 homolog (CcSERK1) was cloned in this work, and its early function in the transcription of embryogenesis master genes and of genes encoding proteins involved in auxin metabolism was investigated by externally manipulating its expression in embryogenic leaf explants, before the appearance of embryogenic structures. Overexpression of CcSERK1 early during embryogenesis caused an increase in the number of somatic embryos when the 55-day process was completed. Suppression of CcSERK1 expression by RNA interference almost abolished somatic embryogenesis. Real time-PCR experiments revealed that the transcription of the CcAGL15, CcWUS, CcBBM, CcPKL, CcYUC1, CcPIN1 and CcPIN4 homologs was modified in direct proportion to the expression of CcSERK1 and that only CcLEC1 was inversely affected by the expression levels of CcSERK1. The expression of the CcYUC4 homolog was induced to more than 80-fold under CcSERK1 overexpression conditions, but it was also induced when CcSERK1 expression was silenced. The level of CcTIR1 was not affected by CcSERK1 overexpression but was almost abolished during CcSERK1 silencing. These results suggest that CcSERK1 co-regulates the induction of somatic embryogenesis in Coffea canephora by early activation of YUC-dependent auxin biosynthesis, auxin transport mediated by PIN1 and PIN4, and probably auxin perception by the TIR1 receptor, leading to the induction of early-stage homeotic genes (CcAGL15, CcWUS, CcPKL and CcBBM) and repression of late-stage homeotic genes (CcLec1).

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“…This phenomenon has been widely utilized to obtain homozygous parental materials in crops (Ferrie and Caswell, 2011; Corral-Martínez and Seguí-Simarro, 2012; Yuan et al, 2015). Several investigations have explored the natural basis of microspore embryogenesis (Belmonte et al, 2010; Rodríguez-Serrano et al, 2012; Solís et al, 2012, 2015; Ahmadi et al, 2016). Microspores or early binucleate pollen grains were demonstrated to more easily undergo embryogenesis in vitro (Keller et al, 1987; Ferrie, 2003).…”

Section: Discussionmentioning

confidence: 99%

Small RNA Sequencing Reveals Differential miRNA Expression in the Early Development of Broccoli (Brassica oleracea var. italica) Pollen

Wang

et al. 2017

Front. Plant Sci.

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Pollen development is an important and complex biological process in the sexual reproduction of flowering plants. Although the cytological characteristics of pollen development are well defined, the regulation of its early stages remains largely unknown. In the present study, miRNAs were explored in the early development of broccoli (Brassica oleracea var. italica) pollen. A total of 333 known miRNAs that originated from 235 miRNA families were detected. Fifty-five novel miRNA candidates were identified. Sixty of the 333 known miRNAs and 49 of the 55 predicted novel miRNAs exhibited significantly differential expression profiling in the three distinct developmental stages of broccoli pollen. Among these differentially expressed miRNAs, miRNAs that would be involved in the developmental phase transition from uninucleate microspores to binucleate pollen grains or from binucleate to trinucleate pollen grains were identified. miRNAs that showed significantly enriched expression in a specific early stage of broccoli pollen development were also observed. In addition, 552 targets for 127 known miRNAs and 69 targets for 40 predicted novel miRNAs were bioinformatically identified. Functional annotation and GO (Gene Ontology) analysis indicated that the putative miRNA targets showed significant enrichment in GO terms that were related to plant organ formation and morphogenesis. Some of enriched GO terms were detected for the targets directly involved in plant male reproduction development. These findings provided new insights into the functions of miRNA-mediated regulatory networks in broccoli pollen development.

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Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

Cited by 27 publications

References 46 publications

Characterization of GhSERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton

Characterization of GhSERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Small RNA Sequencing Reveals Differential miRNA Expression in the Early Development of Broccoli (Brassica oleracea var. italica) Pollen

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