Molecular characterization and expression analysis of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE gene family in Paeonia suffruticosa

Wang, Shunli; Ren, Xiuxia; Xue, Jingqi; Xue, Yuqian; Cheng, Xiaodan; Hou, Xiaogai; Zhang, Xiuxin

doi:10.1007/s00299-020-02573-5

Cited by 12 publications

(13 citation statements)

References 33 publications

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“… 5 Several genes associated with flowering have also been cloned in tree peony, such as GA biosynthesis and signaling genes, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. 19 , 20 The mechanism regulating flowering period in tree peony has not been fully elucidated. 17 A better understanding of the molecular mechanism of flowering time in tree peony could provide a theoretical basis for manipulating flowering time and duration.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed miRNAs and their target genes in response to brassinolide treatment on flowering of tree peony (Paeonia ostii)

Zhang

Song

Guo

et al. 2022

Plant Signaling & Behavior

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Tree peony is a famous flower plant in China, but the short and concentrated flowering period limits its ornamental value and economic value. Brassinolide (BR) plays an important role in plant growth and development including flowering. There have been a large number of reports on the molecular aspects of the flowering process, but the genetic mechanism that was responsible for miRNA-guided regulation of tree peony is almost unclear. In this study, the leaves of tree peony cultivar, ‘Feng Dan’, were sprayed with different concentrations of BR, and the obvious bloom delay was found at the treatment with BR 50 μg/L. The small RNA sequencing and transcriptome sequencing were performed on the petals of tree peony under an untreated control (CK) and the treatment with BR 50 μg/L during four consecutive flowering development stages. A total of 22 known miRNAs belonging to 12 families were identified and 84 novel miRNAs were predicted. Combined with transcriptome data, a total of 376 target genes were predicted for the 18 differentially expressed known miRNAs and 177 target genes were predicted for the 23 differentially expressed novel miRNAs. Additionally, the potential miRNAs and their target genes were identified, including miR156b targeting SPL , miR172a_4 targeting AP2 and four novel miRNAs targeting SPA1 , and revealed that they might affect the flowering time in tree peony. Collectively, these results would provide a theoretical basis for further analysis of miRNA-guided regulation on flowering period in tree peony.

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Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed miRNAs and their target genes in response to brassinolide treatment on flowering of tree peony (Paeonia ostii)

Zhang

Song

Guo

et al. 2022

Plant Signaling & Behavior

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“…Fresh bulb samples were weighted and dried in an oven at 80 °C before reweighing until they attained a constant weight. Hierarchical clustering was performed based on their weight using the MeV (Multi Experiment Viewer) tool ( ) ( Wang et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Hierarchical clustering was performed based on their weight using the MeV (Multi Experiment Viewer) tool (https://www. tm4.org/mev.html) (Wang et al, 2020).…”

Section: Measurements Of the Mass For The Bulbs Leaves And Flowersmentioning

confidence: 99%

“…Flower buds and flowers were collected from 0 d to 70 d, while leaf samples were collected from 14 d to 70 d. The samples were immediately frozen in liquid nitrogen and stored at -80 °C until further analysis. The total RNA from the samples was collected, extracted and reverse transcribed to cDNA synthesis according to Wang et al (2015Wang et al ( , 2020 protocol. The primers for amplifying photosynthesis and flowering-related genes were designed by Primer 5.0 (PRIMER Biosoft, San Francisco, CA, USA).…”

Section: Total Rna Extraction and Gene Expression Analysismentioning

confidence: 99%

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The red/blue light ratios from light-emitting diodes affect growth and flower quality of Hippeastrum hybridum ‘Red Lion’

Wang

Liu

et al. 2022

Front. Plant Sci.

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Light quality strongly impacts the growth and flower quality of ornamental plants. The optimum light quality for the growth and flowering of Hippeastrum remains to be validated. In the present study, we investigated the effect of the red/blue light ratio of LEDs on the growth and flowering quality of H. hybrid ‘Red Lion’. Two LEDs with red/blue light ratio of 1:9 (R10B90) and 9:1 (R90B10) were designed. LEDs of white light were the control. In the earlier vegetative and reproductive growth phase, R90B10 increased the biomass of the bulbs, leaves, and flowers. Compared with the control and R10B90 group, R90B10 LEDs delayed flowering by 2.30 d and 3.26 d, respectively. Based on chlorophyll contents, photosynthetic capacity, chlorophyll fluorescence parameters, and carbohydrate contents, the photosynthesis rate was higher in the R10B90 group. Optimal red and blue light intensity promoted the accumulation of carbohydrates and early flowering and prolonged the flowering period of H. hybrid. Microscopic analysis showed that stomatal density was high, and the number of chloroplasts was large in the R10B90 treatment group, which enhanced photosynthesis. Particularly, R10B90 promoted the expression of seven key genes related to chlorophyll synthesis. R10B90 also promoted early overexpression of the HpCOL gene that promotes early flowering. Thus, higher blue light and 10% red light intensities promote early and extended flowering, while higher red light and 10% blue light promote vegetative plant growth but delay flowering.

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“…Importantly, SPL genes are distributed in a vast majority of green plants, including single-celled algae, mosses, gymnosperms, and angiosperms [4][5][6]. To date, since the completion of genome sequencing and the thorough development of the functional genome, 17, 19, 56, 16, and 14 SPL genes have been systematically identified in A. thaliana, O. sativa, Triticum aestivum, Peaonia suffruticosa, and Paeonia suffruticosa, respectively [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The Mulberry SPL Gene Family and the Response of MnSPL7 to Silkworm Herbivory through Activating the Transcription of MnTT2L2 in the Catechin Biosynthesis Pathway

Luo

et al. 2022

IJMS

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SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, as unique plant transcription factors, play important roles in plant developmental regulation and stress response adaptation. Although mulberry is a commercially valuable tree species, there have been few systematic studies on SPL genes. In this work, we identified 15 full-length SPL genes in the mulberry genome, which were distributed on 4 Morus notabilis chromosomes. Phylogenetic analysis clustered the SPL genes from five plants (Malus × domestica Borkh, Populus trichocarpa, M. notabilis, Arabidopsis thaliana, and Oryza sativa) into five groups. Two zinc fingers (Zn1 and Zn2) were found in the conserved SBP domain in all of the MnSPLs. Comparative analyses of gene structures and conserved motifs revealed the conservation of MnSPLs within a group, whereas there were significant structure differences among groups. Gene quantitative analysis showed that the expression of MnSPLs had tissue specificity, and MnSPLs had much higher expression levels in older mulberry leaves. Furthermore, transcriptome data showed that the expression levels of MnSPL7 and MnSPL14 were significantly increased under silkworm herbivory. Molecular experiments revealed that MnSPL7 responded to herbivory treatment through promoting the transcription of MnTT2L2 and further upregulating the expression levels of catechin synthesis genes (F3′H, DFR, and LAR).

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Molecular characterization and expression analysis of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE gene family in Paeonia suffruticosa

Cited by 12 publications

References 33 publications

Identification of differentially expressed miRNAs and their target genes in response to brassinolide treatment on flowering of tree peony (Paeonia ostii)

Identification of differentially expressed miRNAs and their target genes in response to brassinolide treatment on flowering of tree peony (Paeonia ostii)

The red/blue light ratios from light-emitting diodes affect growth and flower quality of Hippeastrum hybridum ‘Red Lion’

The Mulberry SPL Gene Family and the Response of MnSPL7 to Silkworm Herbivory through Activating the Transcription of MnTT2L2 in the Catechin Biosynthesis Pathway

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