Molecular characterization and expression of two interleukin-17 receptor A genes on different chromosomes in Japanese medaka, Oryzias latipes

Okamura, Yo; Morimoto, Natsuki; Sawada, Shuzo; Kono, Tomoya; Hikima, Jun-ichi; Sakai, Masahiro

doi:10.1016/j.cbpb.2019.110386

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…This study is the first to establish a mutant medaka line of the IL-17RA1 gene using the CRISPR–Cas9 genome-editing system, with comprehensive transcriptomic analyses. In our previous report, we revealed that medaka have two IL-17RA genes (IL-17RA1 and IL-17RA2) encoded on different chromosomes 23 . Synteny analysis suggested that the medaka IL-17RA1 is equivalent to the homologous mammalian IL-17RA gene, and qPCR analysis confirmed remarkably higher expression of IL-17RA1 than of IL-17RA2 in both the anterior and posterior intestines 23 .…”

Section: Discussionmentioning

confidence: 97%

“…In our previous report, we revealed that medaka have two IL-17RA genes (IL-17RA1 and IL-17RA2) encoded on different chromosomes 23 . Synteny analysis suggested that the medaka IL-17RA1 is equivalent to the homologous mammalian IL-17RA gene, and qPCR analysis confirmed remarkably higher expression of IL-17RA1 than of IL-17RA2 in both the anterior and posterior intestines 23 . Thus, we selected IL-17RA1 as the target gene for further exploration in teleosts.…”

Section: Discussionmentioning

confidence: 97%

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A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

Okamura

Miyanishi

Kinoshita

et al. 2021

Sci Rep

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In the intestine, the host must be able to control the gut microbiota and efficiently absorb transiently supplied metabolites, at the risk of enormous infection. In mammals, the inflammatory cytokine interleukin (IL)-17A/F is one of the key mediators in the intestinal immune system. However, many functions of IL-17 in vertebrate intestines remain unclarified. In this study, we established a gene-knockout (KO) model of IL-17 receptor A1 (IL-17RA1, an IL-17A/F receptor) in Japanese medaka (Oryzias latipes) using genome editing technique, and the phenotypes were compared to wild type (WT) based on transcriptome analyses. Upon hatching, homozygous IL-17RA1-KO medaka mutants showed no significant morphological abnormality. However, after 4 months, significant weight decreases and reduced survival rates were observed in IL-17RA1-KO medaka. Comparison of gene-expression patterns in WT and IL-17RA1-KO medaka revealed that various metabolism- and immune-related genes were significantly down-regulated in IL-17RA1-KO medaka intestine, particularly genes related to mevalonate metabolism (mvda, acat2, hmgcs1, and hmgcra) and genes related to IL-17 signaling (such as il17c, il17a/f1, and rorc) were found to be decreased. Conversely, expression of genes related to cardiovascular system development, including fli1a, sox7, and notch1b in the anterior intestine, and that of genes related to oxidation–reduction processes including ugp2a, aoc1, and nos1 in posterior intestine was up-regulated in IL-17RA1-KO medaka. These findings show that IL-17RA regulated immune- and various metabolism-related genes in the intestine for maintaining the health of Japanese medaka.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

Okamura

Miyanishi

Kinoshita

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…This study is the rst to establish a mutant medaka line of the IL-17RA1 gene using the CRISPR-Cas9 genome-editing system, with comprehensive transcriptomic analyses. In our previous report, we revealed that medaka have two IL-17RA genes (IL-17RA1 and IL-17RA2) encoded on different chromosomes 22 . Synteny analysis suggested that the medaka IL-17RA1 is equivalent to the homologous mammalian IL-17RA gene, and qPCR analysis con rmed remarkably higher expression of IL-17RA1 than of IL-17RA2 in both the anterior and posterior intestines 22 .…”

Section: Discussionmentioning

confidence: 97%

“…In our previous report, we revealed that medaka have two IL-17RA genes (IL-17RA1 and IL-17RA2) encoded on different chromosomes 22 . Synteny analysis suggested that the medaka IL-17RA1 is equivalent to the homologous mammalian IL-17RA gene, and qPCR analysis con rmed remarkably higher expression of IL-17RA1 than of IL-17RA2 in both the anterior and posterior intestines 22 . Thus, we selected IL-17RA1 as the target gene for further exploration in teleosts.…”

Section: Discussionmentioning

confidence: 97%

Interleukin-17 Receptor A1 Gene Knockout Causes Weight Loss and Reduction of Intestinal Metabolism-Related Genes in The Japanese Medaka, Oryzias Latipes

Okamura¹,

Miyanishi²,

Kinoshita³

et al. 2020

Preprint

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In the intestine, the host must be able to control the gut microbiota and efficiently absorb transiently supplied metabolites, at the risk of enormous infection. In mammals, the inflammatory cytokine interleukin (IL)-17A/F is one of the key mediators in the intestinal immune system. However, many functions of IL-17 in vertebrate intestines remain unclarified. In this study, we established a gene-knockout (KO) model of IL-17 receptor A1 (RA1), an IL-17A/F receptor, in Japanese medaka (Oryzias latipes) using genome editing technique and the phenotypes were compared to wild type (WT) based on transcriptome analyses. Upon hatching, homozygous IL-17RA1-KO medaka mutants showed no significant morphological abnormality. However, after 4 months, significant weight decreases and reduced survival rates were observed in IL-17RA1-KO medaka. Comparing gene-expression patterns in WT and IL-17RA1-KO medaka revealed that various metabolism- and immune-related genes were significantly down-regulated in IL-17RA1-KO medaka intestine, particularly genes related to mevalonate metabolism (mvda, acat2, hmgcs1, and hmgcra) and genes related to IL-17 signaling (such as il17c, il17a/f1, and rorc) were found to be decreased. These findings show that IL-17RA regulated immune- and various metabolism-related genes in the intestine for maintaining the health of Japanese medaka.

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“…The two FnIII domains are linked together with the IL17RC (heteromeric receptor complex) bind to the IL17A and IL17F molecules (Ely et al, 2009;Liu et al, 2013). These binding complexes trigger the intracellular signaling pathways through the activation of transcription factors of the mitogen-activated protein kinases, nuclear factor-kappa B, and CCAAT enhancer-binding protein (Iwakura et al, 2011;Wang et al, 2014;Okamura et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Association of a non-synonymous SNP of IL17RA gene with litter size traits in Large White and Landrace pigs

Norseeda

Liu

Teltathum³

et al. 2021

VIS

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Interleukin-17 receptor A (IL17RA) is one of the cytokine receptors of the pro-inflammatory interleukin-17 (IL17) cytokine family. The IL17 and IL17RA genes are involved in inflammatory and immune responses as well as reproductive process of mammals. The purposes of this study were to examine polymorphisms in the porcine IL17RA gene and to assess its effects on litter size traits in Large White and Landrace pigs. Three non-synonymous single nucleotide polymorphisms (SNPs) in the porcine IL17RA gene were verified. The porcine IL17RA c.785C>T (p.Ala262Val) was found to be segregating in the Large White and Landrace pigs. No polymorphisms in the coding region of the porcine IL17RA gene at the two non-synonymous SNPs loci of c.997G>A (p.Val333Ile) and c.1962T>G (p.Asp654Glu) were found. The porcine IL17RA c.785C>T polymorphism was significantly associated with the total number born (TNB) and the number born alive (NBA) in Large White pigs (P<0.05). Moreover, the porcine IL17RA c.785C>T was significantly associated with the TNB, NBA, total birth weight (TBW), and total weaning weight of piglets at 21 days (TWW) in Landrace pigs (P<0.05). These results supported the importance of the porcine IL17RA gene in the litter size traits of pigs. Thus, the porcine IL17RA could be used as a potential candidate gene for improving litter size traits in pig breeding.

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Molecular characterization and expression of two interleukin-17 receptor A genes on different chromosomes in Japanese medaka, Oryzias latipes

Cited by 10 publications

References 31 publications

A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

A defective interleukin-17 receptor A1 causes weight loss and intestinal metabolism-related gene downregulation in Japanese medaka, Oryzias latipes

Interleukin-17 Receptor A1 Gene Knockout Causes Weight Loss and Reduction of Intestinal Metabolism-Related Genes in The Japanese Medaka, Oryzias Latipes

Association of a non-synonymous SNP of IL17RA gene with litter size traits in Large White and Landrace pigs

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