Molecular characterization and expression of sensory neuron membrane proteins in the parasitoid <i>Microplitis mediator</i> (Hymenoptera: Braconidae)

Shan, Shuang; Wang, Shan-Ning; Song, Xuan; Khashaveh, Adel; Lu, Ziyue; Dhiloo, Khalid Hussain; Li, Ruijun; Gao, Xiwu; Zhang, Yongjun

doi:10.1111/1744-7917.12667

Cited by 29 publications

(23 citation statements)

References 66 publications

(113 reference statements)

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“…Insect SNMPs play important roles in signal transduction. MmedSNMP2 from M. mediator is not only expressed in antennae, but also in head, legs and other tissues, suggesting the multiple roles of MmedSNMP2 (Shan et al, 2019). AlucSNMP2a identified in legs of A. lucorum may participate in multiple ecological functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum

Zhang

et al. 2020

Front. Physiol.

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Apolygus lucorum (Hemiptera: Miridae), one of the main insect pests, causes severe damage in cotton and many other economic crops. As is well-known, legs play important roles in the chemoreception of insects. In this study, the putative chemosensory proteins in legs of A. lucorum involved in close or contact chemical communication of adult bugs were investigated using RNA transcriptome sequencing and qPCR methods. Transcriptome data of forelegs, middle legs and hind legs of adult bugs demonstrated that 20 odorant binding protein (OBP) genes, eight chemosensory protein (CSP) genes, one odorant receptor (OR) gene, one ionotropic receptor (IR) gene and one sensory neuron membrane protein (SNMP) gene were identified in legs of A. lucorum. Compared to the previous antennae transcriptome data, five CSPs, IR21a and SNMP2a were newly identified in legs. Results of qPCR analysis indicated that all these putative chemosensory genes were ubiquitously expressed in forelegs, middle legs and hind legs of bugs. Furthermore, four types of sensilla on legs of A. lucorum including sensilla trichodea (subtypes: long straight sensilla trichodea, Str1; long curved sensilla trichodea, Str2), sensilla chaetica (subtypes: sensilla chaetica 1, Sch1; sensilla chaetica 2, Sch2; and sensilla chaetica 3, Sch3), sensilla basiconca (subtypes: mediumlong sensilla basiconca, Sba1; short sensilla basiconca, Sba2) and Böhm bristles (BB) were found using scanning electron microscopy. Additionally, the largest number of sensilla was observed on hind legs, while the forelegs had the smallest number of sensilla. Our data provide valuable insights into understanding the chemoreception of legs in A. lucorum.

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Section: Discussionmentioning

confidence: 99%

Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum

Zhang

et al. 2020

Front. Physiol.

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“…SNMP1s have originally been discovered as proteins specific to OSNs of pheromone-sensitive sensilla in the moth Antheraea polyphemus (Rogers et al 1997 ). Accordingly, ISH and IHC investigations of SNMP1 orthologues in other moths (Rogers et al 2001a , Forstner et al 2008 , Gu et al 2013 , Liu et al 2014 ), as well as in flies (Benton et al 2007 ), wasps (Shan et al 2020 ), and locusts (Pregitzer et al 2017 , Pregitzer et al 2019 ), revealed their neuronal expression in OSNs of the antenna.…”

Section: Expression Of Snmps In the Olfactory System And Beyondmentioning

confidence: 99%

The role of SNMPs in insect olfaction

Cassau

Krieger

2020

Cell Tissue Res

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The sense of smell enables insects to recognize olfactory signals crucial for survival and reproduction. In insects, odorant detection highly depends on the interplay of distinct proteins expressed by specialized olfactory sensory neurons (OSNs) and associated support cells which are housed together in chemosensory units, named sensilla, mainly located on the antenna. Besides odorant-binding proteins (OBPs) and olfactory receptors, so-called sensory neuron membrane proteins (SNMPs) are indicated to play a critical role in the detection of certain odorants. SNMPs are insect-specific membrane proteins initially identified in pheromone-sensitive OSNs of Lepidoptera and are indispensable for a proper detection of pheromones. In the last decades, genome and transcriptome analyses have revealed a wide distribution of SNMP-encoding genes in holometabolous and hemimetabolous insects, with a given species expressing multiple subtypes in distinct cells of the olfactory system. Besides SNMPs having a neuronal expression in subpopulations of OSNs, certain SNMP types were found expressed in OSN-associated support cells suggesting different decisive roles of SNMPs in the peripheral olfactory system. In this review, we will report the state of knowledge of neuronal and non-neuronal members of the SNMP family and discuss their possible functions in insect olfaction.

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“…SNMP1 of D. melanogaster is involved in pheromone detection and enhances the Ca 2+ responses served in signal transduction 76 . SNMP1 of M. mediator was determined to participate in both pheromone and general odor detection 77 . In contrast, the general functional mechanism of SNMP2 in parasitoids is still poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Sensory Geneidentificationin the Transcriptome of the Ectoparasitoid Quadrastichus Mendeliinthe Eucalyptus Gall wasp Leptocybe Invasa

Huang¹,

Wang²,

Lu³

et al. 2020

Preprint

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Gall-inducing insects live within plant tissues and induce tumor-like growth that provides insects with food, shelter, and protection from natural enemies. Interestingly, these insects can be precisely targeted by parasitoids. However, the chemical mechanism of the host location by parasitoids of gall-inducing insects has not been elucidated. Empirical evidence has shown that sensory genes play a key role in the host location of parasitoids. To date, the sensory genes that regulate parasitoids to locate gall-inducing insects have not been uncovered. An obligate ectoparasitoid, Quadrastichus mendeli Kim & La Salle (Hymenoptera: Eulophidae: Tetrastichinae), is one of the most important parasitoids of Leptocybe invasa, which is a global gall-making pest in eucalyptus plantations. Therefore, Q. mendeli - L. invasa provides an ideal system to study the way that parasitoids use sensory genes in gall-making pests. In this study, we present the transcriptome of Q. mendeli using high-throughput sequencing. In total, 31820 transcripts were obtained and assembled into 26925 unigenes in Q. mendeli. Then, the major sensory genes were identified, and phylogenetic analyses were performed with these genes from Q. mendeli and other model insect species. Three chemosensory proteins (CSPs), 10 gustatory receptors (GRs), 21 ionotropic receptors (IRs), 58 odorant binding proteins (OBPs), 30 odorant receptors (ORs) and 2 sensory neuron membrane proteins (SNMPs) were identified in Q. mendeli by bioinformatics analysis. Our report is the first to obtain abundant biological information on the transcriptome of Q. mendeli that provided valuable information regarding the molecular basis of Q. mendeli perception, and it may help to understand the host location of parasitoids of gall-making pests.

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Molecular characterization and expression of sensory neuron membrane proteins in the parasitoid Microplitis mediator (Hymenoptera: Braconidae)

Cited by 29 publications

References 66 publications

Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum

Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum

The role of SNMPs in insect olfaction

Sensory Geneidentificationin the Transcriptome of the Ectoparasitoid Quadrastichus Mendeliinthe Eucalyptus Gall wasp Leptocybe Invasa

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