Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Li, Shi-Zhu; Liu, Wei; Li, Zhi; Wang, Yang; Zhou, Li; Yi, Meisheng; Gui, Jian‐Fang

doi:10.1016/j.gene.2016.07.027

Cited by 26 publications

(15 citation statements)

References 42 publications

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“…Early differentiation of gonads and of female germline cells in species that belong to family Cyprinidae has been described in detail in C. carpio (Mazzoni et al, 2010; van Winkoop, Booms, Dulos, & Timmermans, 1992), in the gibel carp Carassius gibelio (Li et al, 2016; Xu, Gui, & Hong, 2005), the black carp Mylopharyngodon piceus (Xue et al, 2017), in D. rerio (Maack & Segner, 2003; Marlow & Mullins, 2008) as well as in the European barbel Barbus barbus (Thiry & Poncin, 2005). The results obtained during this study revealed that early oocyte development in differentiated ovarian follicles of L. marequensis is similar to that of other species of Cyprinidae and other species of Teleostei (Beams & Kessel, 1973; Grier, 2012; Grier, Uribe, & Parenti, 2007; Grier et al, 2018; Kjesbu et al, 2011; Kobayashi & Iwamatsu, 2000; McPherson & Kjesbu, 2012; Selman & Wallace, 1989; Żelazowska & Halajian, 2019).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Asymmetry in the cytoplasm of oocytes of largescale yellowfish Labeobarbus marequensis Smith 1841 (Teleostei: Cypriniformes: Cyprinidae)

Żelazowska

Halajian

2020

Journal of Morphology

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The ovaries of the largescale yellowfish, Labeobarbus marequensis (Teleostei: Cypriniformes: Cyprinidae), are made up of the germinal epithelium, nests of late chromatin nucleolus stage oocytes, and ovarian follicles. Each follicle is composed of a single oocyte, which is surrounded by somatic follicular cells and a basal lamina covered by thecal cells. We describe polarization and ultrastructure of oocytes during the primary growth stage. The oocyte nucleus contains lampbrush chromosomes, nuclear bodies and fibrillar material in which multiple nucleoli arise. Nuage aggregations composed of material of a nuclear origin are present in the perinuclear cytoplasm. The Balbiani body (Bb) contains aggregations of nuage, rough endoplasmic reticulum, individual mitochondria and complexes of mitochondria with nuage (cement). Some mitochondria in the Bb come into close contact with endoplasmic reticulum cisternae and vesicles that contain granular material. At the start of primary growth, the Bb is present in the cytoplasm close to the nucleus. Next, it expands towards the oocyte plasma membrane. In these oocytes, a spherical structure, the so-called yolk nucleus, arises in the Bb. It consists of granular nuage in which mitochondria and vesicles containing granular material are immersed. Later, the Bb becomes fragmented and a fully grown yolk nucleus is present in the vegetal region. It contains numerous threads composed of granular nuage, mitochondria, lysosomelike organelles and autophagosomes. We discuss the formation of autophagosomes in the cytoplasm of primary growth oocytes. During the final step of primary growth, the cortical alveoli arise in the cytoplasm and are distributed evenly. The eggshell is deposited on the external surface of the oocyte plasma membrane and is made up of two egg envelopes that are pierced by numerous pore canals. The external egg envelope is covered in protuberances. During primary growth no lipid droplets are synthesized or stored in the oocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Asymmetry in the cytoplasm of oocytes of largescale yellowfish Labeobarbus marequensis Smith 1841 (Teleostei: Cypriniformes: Cyprinidae)

Żelazowska

Halajian

2020

Journal of Morphology

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“…The subsequent discovery of homologs of the LS-12 genes across a broad range of tropical and subtropical teleost fishes called into question whether the LS-12 protein, and therefore the new class of Type IV AFPs, are actually apolipoproteins that coincidentally have negligible antifreeze properties in polar species, or whether they have different functions altogether (eg, gastrulation; lipid metabolism). 25 - 28 Li et al 29 analyzed the expression of the LS-12-like gene homolog in the gibel carp ( Carassius auratus gibelio ) and found that the gene is predominantly expressed during embryogenesis; and that the protein was no longer detectable in larvae older than 13 days. Deng and Laursen 30 showed that the concentration of the LS-12 gene in the longhorn sculpin, M. octodecimspinosis , is so low that without concentration of the protein, antifreeze activity could not be detected.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics of a Greenlandic Snailfish Reveals Exceptionally High Expression of Antifreeze Protein Transcripts

Burns

Gruber

Gaffney

et al. 2022

Evol Bioinform Online

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Polar fishes have evolved antifreeze proteins (AFPs) that allow them to survive in subzero temperatures. We performed deep transcriptomic sequencing on a postlarval/juvenile variegated snailfish, Liparis gibbus (Actinopterygii: Scorpaeniformes: Cottoidei: Liparidae), living in an iceberg habitat (−2°C) in Eastern Greenland and report detection of highly expressed transcripts that code for putative AFPs from 2 gene families, Type I and LS-12-like proteins (putative Type IV AFPs). The transcripts encoding both proteins have expression levels among the top <1% of expressed genes in the fish. The Type I AFP sequence is different from a reported Type I AFP from the same species, possibly expressed from a different genetic locus. While prior findings from related adult sculpins suggest that LS-12-like/Type IV AFPs may not have a role in antifreeze protection, our finding of very high relative gene expression of the LS-12-like gene suggests that highly active transcription of the gene is important to the fish in the iceberg habitat and raises the possibility that weak or combinatorial antifreeze activity could be beneficial. These findings highlight the physiological importance of antifreeze proteins to the survival of fishes living in polar habitats.

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“…Dnd was initially identified in zebrafish as an RNA-binding protein that is specifically expressed in germ cells causing aberrant PGC migration and germ cell-deficient when blocked by specific antisense morpholinos [ 4 ]. Additionally, homologous dnd genes are characterized in diverse vertebrates, such as mice ( Mus musculus ) [ 5 ], frog ( Xenopus laevis ) [ 6 ], chickens ( Gallus gallus ) [ 7 ], medaka ( Oryzias latipes ) [ 8 ], Chinese sturgeon ( Acipenser sinensis ) [ 9 ], gibel carp ( Carassius Gibelio ) [ 10 ], orange-spotted grouper ( Epinephelus coioides ) [ 11 ] and Celebes medaka ( Oryzias celebensis ) [ 12 ]. All these studies suggest that dnd is primarily expressed in PGCs during embryogenesis, but it is shown sexually dimorphic in adult gonads across species.…”

Section: Introductionmentioning

confidence: 99%

“…In mice, dnd is only detectable in testis [ 5 ], whereas it is restricted to the ovary in frog [ 6 ]. Dnd occurs in germ cells of both sexes in medaka [ 8 ], zebrafish ( Danio rerio ) [ 4 ], carp ( Carassius gibelio ) [ 10 ], turbot ( Scophthalmus maximus ) [ 13 ] and starry flounder ( Platichthys stellatus ) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Spinyhead Croaker Germ Cells Gene dnd Visualizes Primordial Germ Cells in Medaka

Liu

Wen

et al. 2022

Life

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Spinyhead croaker (Collichthys lucidus) is an economically important fish suffering from population decline caused by overfishing and habitat destruction. Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conservation of the involved species. Dead end (dnd) gene plays an indispensable role in PGC specification, maintenance, and development. In the current study, we report the cloning and expression patterns of dnd in C. lucidus (Cldnd). RT-PCR analysis revealed that Cldnd was specifically expressed in both sexual gonads. In the ovary, Cldnd RNA was uniformly distributed in the oocytes and abundant in oogonia, and gradually decreased with oogenesis. A similar expression pattern was also detected in testis. Dual fluorescent in situ hybridization of Cldnd and Clvasa demonstrated that they almost had the same distribution except in oocytes at stage I, in which the vasa RNA aggregated into some particles. Furthermore, Cldnd 3′ UTR was sufficient to guide the Green Fluorescent Protein (GFP) specifically and stably expressed in the PGCs of medaka. These findings offer insight into that Cldnd is an evolutionarily conserved germline-specific gene and even a potential candidate for PGC manipulation in C. lucidus.

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Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Cited by 26 publications

References 42 publications

Asymmetry in the cytoplasm of oocytes of largescale yellowfish Labeobarbus marequensis Smith 1841 (Teleostei: Cypriniformes: Cyprinidae)

Asymmetry in the cytoplasm of oocytes of largescale yellowfish Labeobarbus marequensis Smith 1841 (Teleostei: Cypriniformes: Cyprinidae)

Transcriptomics of a Greenlandic Snailfish Reveals Exceptionally High Expression of Antifreeze Protein Transcripts

Spinyhead Croaker Germ Cells Gene dnd Visualizes Primordial Germ Cells in Medaka

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