Molecular characterization and expression profiles of two interleukin genes IL-8 and IL-10 in Pacific cod (Gadus macrocephalus)

Jiang, Chen; Yang, Xu; Xu, Hao; Mao, Ming; Chen, Bing; Yang, Zhen; Guan, Yude; Wang, He

doi:10.1016/j.aqrep.2021.100788

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…Larvae fed with TG and LG diets had signifcantly lower mRNA expression of tnf-α which is an important proinfammatory factor. Moreover, il-10 is an anti-infammatory cytokine produced mainly by macrophages and plays a role in counteracting and controlling excessive infammatory response [47][48][49]. In the present study, the mRNA expression of il-10 signifcantly increased with the larvae fed with TG and LG diets.…”

Section: Discussionsupporting

confidence: 55%

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Liu

Wang

Zhang

et al. 2023

Aquaculture Research

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A 30-day feeding experiment was designed to investigate the effects of different adhesives on survival, growth performance, digestive enzyme activities, antioxidant capacity, and inflammation response of large yellow croaker (Larimichthys crocea) larvae (initial weight 8.40 ± 0.18 mg). Four iso-nitrogenous (55% crude protein) and iso-lipidic (22% crude lipid) microbound diets (MBD) were formulated to contain 2% sodium alginate (SA), 2% tragacanth gum (TG), 2% linseed gum (LG), and 2% poloxamer (PX), respectively. Results showed that larvae fed with TG and LG diets had significantly higher survival rate than the larvae fed with SA and PX ( P < 0.05 ). Larvae fed with the PX diet had lower final weight and specific growth rate than the other groups ( P < 0.05 ). Larvae fed with TG diet had significant higher activities of lipase in pancreatic segments and trypsin in intestinal segments than the larvae fed with the PX ( P < 0.05 ). Meanwhile, the lipase activities of larvae fed with LG was significantly higher in intestinal segments than those fed with SA and PX diets ( P < 0.05 ). Larvae fed with TG and LG diets had significantly higher activities of AKP in brush border membranes than larvae fed with SA and PX diets ( P < 0.05 ). Moreover, the mRNA expression of pcna and zo-1 increased, respectively, in larvae fed TG and LG diets compared with the other groups ( P < 0.05 ). Larvae fed with LG and TG diets had significantly higher activities of SOD and T-AOC than those fed with PX diet ( P < 0.05 ). The content of GSH increased significantly in larvae fed with SA, TG, and LG diets compared with PX treatment ( P < 0.05 ). The activities of iNOS significantly increased in larvae fed with TG and LG diets compared to other groups ( P < 0.05 ). Meanwhile, the mRNA expression of il-10 significantly increased in TG and LG groups ( P < 0.05 ). In conclusion, results of the study demonstrated that TG and LG can be used as feed binders for microdiets with positive effects on survival, activities of digestive enzymes, antioxidant capacity, and inflammatory response. However, PX inhibited the survival and growth, and further study is needed to determine the suitable usage and dosage.

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Section: Discussionsupporting

confidence: 55%

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Liu

Wang

Zhang

et al. 2023

Aquaculture Research

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“…Once the cellular homeostasis is interrupted by diseases or tissue damage, interleukins trigger immediate immune responses against this emergent stressor. However, excessive interleukin synthesis has pathological consequences such as serious systemic inflammatory response and immune diseases (69). Wang et al (70) documented similar inflammatory indications, verified by a higher level of IL-1β expression in hybrid snakehead fish exposed to Nano\MPs and cadmium.…”

Section: Discussionmentioning

confidence: 91%

Toxicity of co-exposure of microplastics and lead in African catfish (Clarias gariepinus)

Soliman,

Salaah,

Hamed

et al. 2023

Front. Vet. Sci.

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Microplastics (MPs) are an emerging threat to freshwater ecosystems with several ecotoxicological ramifications for fish. Microplastics (MPs) can adsorb heavy metals on their surfaces and increase their availability to aquatic organisms. The combined impact of lead and microplastics on fish has only been studied seldom utilizing a variety of markers. The present study aimed to evaluate the hematological, biochemical, and inflammatory signals (cytokines), as well as antioxidant enzymes in African catfish (Clarias gariepinus) exposed to lead (Pb) and MPs individually and combined for 15 days (acute toxicity experiment). The fish were split into four groups, the first of which was the control group. The second group received exposure to 1 mg/L of lead nitrate [Pb(NO3)2]. The third group was given 100 mg/L of MPs. A solution containing 100 mg/L of MPs and 1 mg/L of lead nitrate [Pb(NO3)2] was administered to the fourth group (the combination group). According to the findings, when MPs and Pb were combined for 15 days, the red blood cells (RBCs), thrombocytes, and lymphocytes were significantly reduced in comparison to the control fish. When compared to the control fish, the fish exposed to MPs and Pb alone or together showed a significant rise in blood interleukin-1β (IL-1β) and interleukin-6 (IL-6) cytokines. Both MPs and Pb exposure in catfish resulted in significant changes in the plasma electrolytes. The fish treated with MPs and Pb individually or in combination showed significant reduction in superoxide dismutase (SOD) and total antioxidant capacity (TAC) levels compared to the control group. The fish exposed to the combined action of MPs and Pb showed a considerable modification in all biochemical markers. The difference in the mean concentration of Pb (mg/L) between the fish exposed to Pb alone and the fish subjected to Pb and MPs combination was not statistically significant. In conclusion, according to this investigation, exposure to Pb caused an insignificant increase in Pb accumulation when MPs were present. However, co-exposure may result in anemia, cellular harm, extremely high levels of oxidative stress, and an inflammatory reaction.

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“…Similar inflammatory indications found in earlier studies [ 56 ] confirmed by an increased expression level of IL-1β gene in snakehead fish after exposure to nano-microplastics and cadmium and in C. gariepinus exposed to microplastics and lead [ 57 ]. Uncontrolled production of pro-inflammatory cytokines leads to serious systemic inflammatory response with pathological consequences [ 58 ] which was confirmed in the current histological alterations of vital organs.…”

Section: Discussionmentioning

confidence: 69%

Polystyrene nanoplastic and engine oil synergistically intensify toxicity in Nile tilapia, Oreochromis niloticus

Sayed,

Emeish,

Bakry

et al. 2024

BMC Vet Res

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Polystyrene nanoplastic (PS-NPs) and Engine oil (EO) pose multiple ecotoxic effects with increasing threat to fish ecosystems. The current study investigated the toxicity of 15 days exposure to PS-NPs and / or EO to explore their combined synergistic effects on Nile tilapia, Oreochromis niloticus (O. niloticus). Hematobiochemical parameters, proinflammatory cytokines, and oxidative stress biomarkers as well as histological alterations were evaluated. The experimental design contained 120 acclimated Nile tilapia distributed into four groups, control, PS-NPs (5 mg/L), EO (1%) and their combination (PS-NPs + EO). After 15-days of exposure, blood and tissue samples were collected from all fish experimental groups. Results indicated that Nile tilapia exposed to PS-NPs and / or EO revealed a significant decrease in almost all the measured hematological parameters in comparison to the control, whereas WBCs and lymphocyte counts were significantly increased in the combined group only. Results clarified that the combined PS-NPs + EO group showed the maximum decrease in RBCs, Hb, MCH and MCHC, and showed the maximum significant rise in interleukin-1β (IL-1β), and interleukin-6 (IL-6) in comparison to all other exposed groups. Meanwhile, total antioxidant capacity (TAC) showed a significant (p < 0.05) decline only in the combination group, whereas reduced glutathione (GSH) showed a significant decline in all exposed groups in comparison to the control. Both malondialdehyde (MDA) and aspartate aminotransferase (AST) showed a significant elevation only in the combination group. Uric acid showed the maximum elevation in the combination group than all other groups, whereas creatinine showed significant elevation in the EO and combination group when compared to the control. Furthermore, the present experiment proved that exposure to these toxicants either individually or in combination is accompanied by pronounced histomorpholgical damage characterized by severe necrosis and hemorrhage of the vital organs of Nile tilapia, additionally extensively inflammatory conditions with leucocytes infiltration. We concluded that combination exposure to both PS-NPs and EO caused severe anemia, extreme inflammatory response, oxidative stress, and lipid peroxidation effects, thus they can synergize with each other to intensify toxicity in fish.

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Molecular characterization and expression profiles of two interleukin genes IL-8 and IL-10 in Pacific cod (Gadus macrocephalus)

Cited by 5 publications

References 45 publications

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Tragacanth Gum and Linseed Gum as Adhesives Improved the Survival, Digestive Function, Antioxidant Enzyme Activities, and Immunity in Large Yellow Croaker (Larimichthys crocea) Larvae

Toxicity of co-exposure of microplastics and lead in African catfish (Clarias gariepinus)

Polystyrene nanoplastic and engine oil synergistically intensify toxicity in Nile tilapia, Oreochromis niloticus

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