Molecular characterization and functional analysis of a novel C-type lectin receptor-like gene from a teleost fish, Plecoglossus altivelis

Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages

“…The bactericidal assay for mudskipper kidney-derived MO/MΦ was modified from a previously described method [31]. A standard curve was generated with the RT-qPCR results to assess the bacterial number.…”

Molecular characterization of a transmembrane C-type lectin receptor gene from ayu (Plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages

“…The expression profile of PaCTLRC differed from the two previously reported ayu CTLRs. aCLR mRNA was mainly expressed in the head kidney and PBLs (Chen et al, 2010), while PaCD209L was mainly expressed in the brain and heart (Yang et al, 2015). The mRNA expression of all three ayu CTLRs responded rapidly to pathogen infection.…”

“…1) indicates that PaCTLRC can bind to mannose in a Ca 2+ -dependent manner. PaCTLRC differs from the other two ayu CTLRs, aCLR (Chen et al, 2010) and PaCD209L (Yang et al, 2015), not only in sequence identity but also in protein structure (Fig. 2).…”

Molecular Characterization and Biological Effects of a C-Type Lectin-Like Receptor in Large Yellow Croaker (Larimichthys crocea)

“…Studies on the tissue expression of fish CTLRs under normal or stimulated conditions have been reported [ 10 , 12 , 13 , 16 , 17 ]. In Atlantic salmon, three CTLR (CTLRA, B, and C) genes were expressed in most tissues of healthy fish, and their transcriptional levels were upregulated in the liver post-infection with A. salmonicida [ 10 ].…”

“…At present, many fish CTLR genes have been identified, such as natural killer cell CTLR genes in cichlid fish ( Paralabidochromis chilotes ) and Nile tilapia ( Oreochromis niloticus ) [ 9 ], three kinds of CTLR (CTLR A, B, and C) genes in Atlantic salmon ( Salmo salar ) [ 10 ], lectin-like receptor genes in zebrafish ( Danio rerio ) [ 11 , 12 ], mannose receptor (MR) genes in blunt snout bream ( Megalobrama amblycephala ) and grass carp ( Ctenopharyngodon idella ) [ 13 , 14 ], and three CTLR-like genes in ayu ( Plecoglossus altivelis ) [ 15 , 16 , 17 ]. In ayu, a CTLR-like protein PaCD209L could bind Gram-negative and Gram-positive bacteria in the absence of Ca 2 + and plays a role in the regulation of the phagocytosis and bacterial killing of monocytes/macrophages (MO/Mϕ) [ 16 ], while another CTLR (PaCTLRC) agglutinates several Gram-negative and Gram-positive bacteria in a Ca 2+ -dependent manner [ 17 ]. Blunt snout bream MR has been found to mediate phagocytosis of bacteria in macrophages in a Ca 2+ -dependent manner [ 14 ].…”