Molecular characterization and functional analysis of the vitellogenin receptor from eri silkworm, Samia ricini

Thomas, Dyna Susan; Varma, Meenakshi; Manoharan, Chitra; Rao, Guru Prasad; Vijayan, K.; Mishra, Rakesh; Gopalapillai, Ravikumar; Sinha, Deepak K.

doi:10.1016/j.cbpb.2020.110417

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…In S. exigua , Vg ( SeVg ) and Vg receptor ( SeVgR ) are the key genes associated with yolk synthesis and accumulation. Vg is the main nutrient for egg development in pests, and the vitellogenin receptor plays a key role in the uptake of SeVg by oocytes and transport to the ovary ( Zhao et al, 2018 ; Thomas et al, 2020 ; Hafeez et al, 2021 ; Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The main component of the aphid alarm pheromone (E)-β-farnesene affects the growth and development of Spodoptera exigua by mediating juvenile hormone-related genes

Sun

Zhang

et al. 2022

Front. Plant Sci.

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The (E)-β-farnesene (EβF) is one of the most important secondary metabolites in some plants and provides indirect defense against aphids. However, the direct effect of EβF against pests is still unclear. In this study, various concentrations of EβF (0.16, 0.8, and 4 g/kg) were provided in an artificial diet to determine the direct effects of EβF on Spodoptera exigua. The results showed that an artificial diet containing 4 g/kg of EβF reduced the final survival of the S. exigua larvae and per female fecundity of adults significantly when compared with CK and SC controls (p < 0.05), then ultimately it also significantly affected the intrinsic rate of increase (p < 0.05). Furthermore, the results of the EβF bioassay in an artificial diet also indicated that the proliferation of the S. exigua population was inhibited by the ingestion of EβF in a dose-dependent manner. Combined differential RNA-seq data and RT-qPCR analysis, it was found that four key genes involved in juvenile hormone degradation significantly upregulated in S. exigua larvae treated by EβF at a dose of 0.8 and 4 g/kg when compared with two controls (p < 0.05). This indicated that EβF could disturb the normal function of juvenile hormones and reduce the survival rate of S. exigua larvae. Additionally, two key genes that regulate per fecundity of S. exigua females, including SeVg and SeVgR, were significantly downregulated in adult females (p < 0.05) when they were treated with 0.8 and 4 g/kg of EβF at the larval stage, relative to the expression of these genes after treatment with controls. These findings suggested that EβF first disturbed the normal function of juvenile hormone by upregulating key degradation genes, and then inhibited the expression of SeVg/SeVgR genes and proteins, thus reducing the population size of S. exigua by increasing larval mortality and inhibiting per female fecundity.

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Section: Discussionmentioning

confidence: 99%

The main component of the aphid alarm pheromone (E)-β-farnesene affects the growth and development of Spodoptera exigua by mediating juvenile hormone-related genes

Sun

Zhang

et al. 2022

Front. Plant Sci.

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“…The down-regulated expression of VgR would disrupt ovary development, resulting in malformed ovarioles and abnormal development of eggs. 53 Zhang et al 54 found that the decreased expression level of AhVgR inhibited yolk protein deposition in the ovaries, drastically reducing egg production. Shu et al 31 discovered that the decreased expression of VgR led to low Vg deposition in the ovary and the failure of insect oviposition.…”

Section: Discussionmentioning

confidence: 99%

The fitness advantages of bistrifluron resistance related to chitin synthase A in Spodoptera litura (Fab.) (Noctuidae: Lepidoptera)

Gong

Wang

Huang

et al. 2021

Pest Management Science

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BACKGROUND: Spodoptera litura is one of the major agricultural pests in China, and it has developed serious resistance to many traditional chemical insecticides. In the present study, the bistrifluron-resistant (Bis-SEL) strain accompanied by a higher oviposition, 113.8-fold RR compared to the bistrifluron-susceptible (Bis-UNSEL) strain, was obtained by bidirectional screening. A comparison of their gonad coefficiency and genes related to oviposition or resistance was used to elucidate the resurgence mechanism.RESULTS: The ovarian index, oviposition, and potential egg production in the Bis-SEL strain of female adults were significantly higher than those in the Bis-UNSEL strain, and the length of ovariole in the Bis-SEL strain was also significantly elongated. The protein contents of vitellogenin (Vg) and vitellogenin receptor (VgR) in the Bis-UNSEL strain were lower than those in the Bis-SEL strain, consistent with their gene expressions levels, and there was a significantly positive linear correlation between Vg and VgR protein contents, further confirming that resistant strains have high reproductive fitness. Moreover, the chitin synthase A in the Bis-SEL strain was clearly up-regulated, and a mutation (H866Y) near the QRRRW in the catalytic domain caused a rise in the hydrogen bond between UDP-GlcNAc and chitin synthase, and its chitin content was higher than that in the Bis-UNSEL strain. Nevertheless, the sensitivity of the Bis-SEL strain to bistrifluron was significantly recovered when it was knocked down though RNA interference. CONCLUSION:The fitness advantages of bistrifluron resistance may be related to the up-regulation and mution of chitin synthase A.

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“…VgR belongs to low‐density lipoprotein receptor superfamily (Raikhel & Dhadialla, 1992; Sappington & Raikhel, 1998), and plays indispensible role in vitellogenesis (Cong et al, 2015; Shang et al, 2018). Knockdown VgR by RNAi or knockout VgR by Crispr/cas9 in many insects produces similar phenotypes such as reduced Vgs in ovary, accumulation of Vgs in hemolymph and reduced fecundity (Du et al, 2019; Hu et al, 2019; Husain et al, 2020; Liu et al, 2019; Lu et al, 2015; Miao et al, 2020; Mitchell et al, 2007; Peng et al, 2019; Shang et al, 2018; Shu et al, 2016; Thomas et al, 2020; Tufail & Takeda, 2005; Xiang et al, 2019), suggesting a conserved role for VgR in insects. One VgR exists in locust and is expressed primarily in ovary (Ferenz, 1993).…”

Section: Discussionmentioning

confidence: 99%

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized LmDDX3 expression in female adult locust and analyzed its function in oocyte development. LmDDX3 was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. LmDDX3 RNAi led to a reduced vitellogenin (Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of vitellogenin receptor (VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for LmDDX3 in regulating the transcription of Vg and VgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.

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Molecular characterization and functional analysis of the vitellogenin receptor from eri silkworm, Samia ricini

Cited by 7 publications

References 34 publications

The main component of the aphid alarm pheromone (E)-β-farnesene affects the growth and development of Spodoptera exigua by mediating juvenile hormone-related genes

The main component of the aphid alarm pheromone (E)-β-farnesene affects the growth and development of Spodoptera exigua by mediating juvenile hormone-related genes

The fitness advantages of bistrifluron resistance related to chitin synthase A in Spodoptera litura (Fab.) (Noctuidae: Lepidoptera)

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

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