Molecular characterization and functional analysis of the Halloween genes and CYP18A1 in Bemisia tabaci MED

Liu, Shaonan; He, Chao; Liang, Jinjin; Su, Qi; Hua, Dengke; Wang, Shaoli; Wu, Qingjun; Xie, Wen; Zhang, Youjun

doi:10.1016/j.pestbp.2020.104602

Cited by 20 publications

(33 citation statements)

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“…S1). CYP18A1 played a controlling role in 20-hydroxyecdysone inactivation in B. mori 52 , and were reported to function in development, especially to regulate dimorphic metamorphosis via by insect hormones 53 , 54 . FoLDH could induce oxidation of farnesol to farnesal and produce the second branch of JH III in Pl.…”

Section: Discussionmentioning

confidence: 99%

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Wang

et al. 2022

Sci Rep

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Neuropeptides and neuropeptide receptors are crucial regulators to insect physiological processes. The 21.0 Gb bases were obtained from Illumina sequencing of two libraries representing the female and male heads of Phauda flammans (Walker) (Lepidoptera: Phaudidae), which is a diurnal defoliator of ficus plants and usually outbreaks in the south and south-east Asia, to identify differentially expressed genes, neuropeptides and neuropeptide receptor whose tissue expressions were also evaluated. In total, 99,386 unigenes were obtained, in which 156 up-regulated and 61 down-regulated genes were detected. Fifteen neuropeptides (i.e., F1b, Ast, NP1, IMF, Y, BbA1, CAP2b, NPLP1, SIF, CCH2, NP28, NP3, PDP3, ARF2 and SNPF) and 66 neuropeptide receptor genes (e.g., A2-1, FRL2, A32-1, A32-2, FRL3, etc.) were identified and well-clustered with other lepidopteron. This is the first sequencing, identification neuropeptides and neuropeptide receptor genes from P. flammans which provides valuable information regarding the molecular basis of P. flammans.

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Section: Discussionmentioning

confidence: 99%

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Wang

et al. 2022

Sci Rep

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“…For instance, the five Halloween genes ( CYP307A1 , CYP306A1 , CYP302A1 , CYP315A1 and CYP314A1 ) were first identified in Drosophila melanogaster , and their mutations resulted in very low ecdysteroid titer and similar lethal embryonic phenotypes, including disruptions in head involution, dorsal closure and a failure to produce embryonic cuticle 9–11 . In Hemiptera and Lepidoptera, RNA interference (RNAi) and 20E rescue experiments showed that most of the Halloween genes were necessary and played a crucial role in the development of nymphs or larvae 13–18 . Moreover, in Schistocerca gregaria (Orthoptera), silencing of SgCYP307A1 , SgCYP306A1 and SgCYP314A1 caused a reduction in 20E titer, 19,20 and knockdown of SgCYP307A1 interfered with nymphal development and metamorphosis 21 .…”

Section: Introductionmentioning

confidence: 99%

“…At present, RNAi, a tool to study gene function, has been developed as a new pest management tool 27 . Studies have been reported that targeting insect ecdysteroid metabolism genes could block the metamorphosis effectively and provide useful information for evaluating the efficacy of biopesticides 18 …”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11] In Hemiptera and Lepidoptera, RNA interference (RNAi) and 20E rescue experiments showed that most of the Halloween genes were necessary and played a crucial role in the development of nymphs or larvae. [13][14][15][16][17][18] Moreover, in Schistocerca gregaria (Orthoptera), silencing of SgCYP307A1, SgCYP306A1 and SgCYP314A1 caused a reduction in 20E titer, 19,20 and knockdown of SgCYP307A1 interfered with nymphal development and metamorphosis. 21 Similarly, silencing of LmCYP307A2 in Locusta migratoria resulted in severe molting delays and molting failure, which was rescued by supplementary 20E.…”

Section: Introductionmentioning

confidence: 99%

“…27 Studies have been reported that targeting insect ecdysteroid metabolism genes could block the metamorphosis effectively and provide useful information for evaluating the efficacy of biopesticides. 18 In this study, we identified seven cytochrome P450 genes related to 20E metabolism in SHB and analyzed their developmental and tissue-specific expression patterns. Furthermore, using RNAi and rescue experiments, we determined that AtCYP307A1 is essential for larval-pupal transition.…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of CYP307A1 as a molecular target for controlling the small hive beetle, Aethina tumida

et al. 2022

Pest Management Science

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Background: The molting hormone 20-hydroxyecdysone (20E) plays a key role in insect development, metamorphosis, and reproduction. Previous studies have shown that ecdysteroid metabolism is regulated by a series of CYP genes in most of the insect species. However, the roles of these CYP genes in a Coleopteran beetle, Aethina tumida (small hive beetle, SHB) have not yet been explored.Results: In the current study, we identified seven CYP genes (six Halloween genes and one AtCYP18A1 gene) related to 20E metabolism. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) showed that AtCYP307A1 and AtCYP307B1 were primarily expressed in the embryonic stage and in the cephalothorax of larvae. RNA interference (RNAi) screening revealed that suppression of AtCYP307A1 expression caused a lethal phenotype during the larval-pupal metamorphosis. Furthermore, Hematoxylin and Eosin staining of the integument showed that the RNAi of AtCYP307A1 inhibited the apolysis and degradation of the old cuticle. In addition, silencing of AtCYP307A1 resulted in significant down-regulation of 20E titers and the expression levels of 20E signaling pathway genes. Finally, the AtCYP307A1 RNAi phenotype was rescued by topical application of 20E.Conclusion: Our studies suggest that AtCYP307A1 involved in 20E synthesis is indispensable during the larval-pupal metamorphosis of beetles, which could serve as a putative insecticide target for pest control.

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Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

Zhang,

Tan,

Jin

et al. 2024

Arch Insect Biochem Physiol

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In insects, the expression of 20E response genes that initiate metamorphosis is triggered by a pulse of 20‐hydroxyecdysone (20E). The 20E pulse is generated through two processes: synthesis, which increases its level, and inactivation, which decreases its titer. CYP18A1 functions as an ecdysteroid 26‐hydroxylase and plays a role in 20E removal in several representative insects. However, applying 20E degradation activity of CYP18A1 to other insects remains a significant challenge. In this study, we discovered high levels of Hvcyp18a1 during the larval and late pupal stages, particularly in the larval epidermis and fat body of Henosepilachna vigintioctopunctata, a damaging Coleopteran pest of potatoes. RNA interference (RNAi) targeting Hvcyp18a1 disrupted the pupation. Approximately 75% of the Hvcyp18a1 RNAi larvae experienced developmental arrest and remained as stunted prepupae. Subsequently, they gradually turned black and eventually died. Among the Hvcyp18a1‐depleted animals that successfully pupated, around half became malformed pupae with swollen elytra and hindwings. The emerged adults from these deformed pupae appeared misshapen, with shriveled elytra and hindwings, and were wrapped in the pupal exuviae. Furthermore, RNAi of Hvcyp18a1 increased the expression of a 20E receptor gene (HvEcR) and four 20E response transcripts (HvE75, HvHR3, HvBrC, and HvαFTZ‐F1), while decreased the transcription of HvβFTZ‐F1. Our findings confirm the vital role of CYP18A1 in the pupation, potentially involved in the degradation of 20E in H. vigintioctopunctata.

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Molecular characterization and functional analysis of the Halloween genes and CYP18A1 in Bemisia tabaci MED

Cited by 20 publications

References 50 publications

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

Identification and characterization of CYP307A1 as a molecular target for controlling the small hive beetle, Aethina tumida

Molecular characterization of the cytochrome P450 enzyme CYP18A1 in Henosepilachna vigintioctopunctata

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