Molecular characterization and functional analysis of the OsPsbR gene family in rice

Abstract

Low temperature may exert a negative impact on agronomical productivity. PsbR was known as the 10 kDa Photosystem II polypeptide. Although plant PsbR is thought to play important roles in photosynthesis, little is known about the contribution of plant PsbR to abiotic stress resistance. The expression patterns of three OsPsbR gene family members, OsPsbR1, OsPsbR2, and OsPsbR3, were characterized in rice 'Nipponbare'. Under normal condition, OsPsbR1 and OsPsbR3 showed tissue-specific expression, while the expression of OsPsbR2 could not be detected in all tested tissues. OsPsbR1 was upregulated in response to cold stress, and downregulated under drought, salt, or heat conditions. The upregulation of OsPsbR3 was observed under the treatment of ABA, and its downregulation was detected under drought or heat conditions. Upregulation of OsPsbR1 in rice resulted in significantly increased resistance to cold, but did not affect the yield of rice. Furthermore, after 8 h cold-stress treatment, the expression levels of three cold stress-induced marker genes were significantly higher in the overexpression lines L11 and L19 in comparison with the wild type. All these results suggest that OsPsbR1 may play key roles in photosynthesis and cold stress response and thus has the potential to improve cold stress tolerance of crops.

Genome-wide identification and analyses of the rice OsDUF936 family

“…At the emergence of the fourth leaf, the seedlings of rice Nipponbare were subjected to various stresses and ABA treatment as described previously [36]. For high salinity treatment, NaCl solution was added to achieve a final concentration of about 200 mmol/L.…”

“…Then, the total RNA was treated with RNasefree DNase I (Invitrogen, Carlsbad, CA, USA) for 15 min to degrade any potentially contaminated DNA and the resulting RNA was used for first-strand cDNA synthesis. Real-time PCR was performed as described [36]. The rice Actin1 gene (LOC_Os03g50885) was used as an internal control with primers 5'-TGGCATCTCTCAGCACATTC-3' and 5'-TGCACAATGGATGGGCCAGA-3'.…”

Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass

“…Here we identified and functionally characterized three switchgrass promoters that were highly green tissue-specific and light-regulated. Their expression patterns were very similar to those of their rice homologs [ 53 – 57 ]. To our best knowledge, these are the first characterized green tissue-specific promoters from switchgrass.…”

“…The IRGSP gene IDs of the five plant-type rice PEPC genes Osppc1 , 2a , 2b , 3, and 4 were Os02g0244700, Os08g0366000, Os09g0315700, Os01g0758300, and Os01g0208700, respectively [ 56 ]. The IRGSP gene IDs of the three rice PsbR genes OsPsbR1, 2 , and 3 were Os07g05360, Os07g05365, and Os08g10020, respectively [ 53 ]. The deduced amino acid sequences of the switchgrass homologs of these rice genes were aligned together with their respective query protein sequences using ClustalX 2.0 [ 62 ] (Additional file 1 : Fig.…”

Identification of Sugarcane Host Factors Interacting with the 6K2 Protein of the Sugarcane Mosaic Virus

“…PsbR is involved in binding PsbP [92], and is essential for the optimal oxygen-evolving activity of PSII [47,93,94]. PsbR is required for the binding of LHCSR3 to PSII-LHCII supercomplexes, the efficiency of NPQ in Chlamydomonas reinhardtii [95], and the tolerance to cold stress in rice [96]. VTE3 is involved in a key methylation step in both vitamin E and plastoquinone synthesis [97].…”