Molecular characterization and functional analysis of serine/threonine protein phosphatase of Toxocara canis

Ma, Guangxu; Zhou, Rong Qiong; Hu, Shi Jun; Huang, Han; Zhu, Tao; Xia, Qing

doi:10.1016/j.exppara.2014.03.019

Cited by 9 publications

(8 citation statements)

References 39 publications

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“…A variety of putative PP1r molecules have been identified (Watanabe et al, 2001;Ceulemans and Bollen, 2004;Shi, 2009;Heijman et al, 2013), which have contributed to investigations of PP1 function. Specifically, the important roles of male-specific stp1 in spermatogenesis of nematodes have been investigated in several worms (Boag et al, 2000;Hu et al, 2007;Campbell et al, 2010;Ma et al, 2014); however, the precise molecular mechanisms remain largely unknown. In the current study, a leucine-rich repeat protein cDNA sequence, TcM-1309, obtained from a cDNA library of male adult T. canis was cloned and characterized, from which leucine-rich repeat protein structures were found, corresponding to the consensus of mouse PP1r7 LxxLxLxxNxIxxIxxLxxL and human sds22 ExLxxLxxLxxLxLxxNxIxxI (Renouf et al, 1995;Wang and Sperry, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Pre-immune serum was used as a control. Polyclonal antibody and pre-immune serum used in this experiment were produced in our previous study (Ma et al, 2014). Fluorescence was detected using a fluorescence imaging microscope (Olympus Corporation, Tokyo, Japan).…”

Section: Indirect Fluorescence Immunocytochemistrymentioning

confidence: 99%

“…Specifically, the functional roles of the male-specific serine/threonine protein phosphatase 1 (PP1) gene (stp-1) in reproduction have been investigated in Oesophagostomum dentatum (Boag et al, 2000;Boag et al, 2003), Trichostrongylus vitrinus, Haemonchus contortus and T. canis (Hu et al, 2007;Campbell et al, 2010;Ma et al, 2014), indicating potential roles of stp-1 in spermatogenesis of nematodes. However, functional PP1 requires formation of a complex composed of a catalytic subunit (PP1c) with PP1-binding regulatory subunits (PP1r) (Barford et al, 1998;Heijman et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Zhou

Song

et al. 2015

Acta Tropica

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Section: Discussionmentioning

confidence: 99%

Section: Indirect Fluorescence Immunocytochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Zhou

Song

et al. 2015

Acta Tropica

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“…Among them, serine/threonine-protein phosphatase (STPs) was identified as an important interacting protein. STPs from various parasites have been functionally characterized [ 93 – 95 ]. Protein phosphatases are involved in major biological processes such as cell division, apoptosis and exocytosis [ 96 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles

Gadahi¹,

Wang²,

Gao³

et al. 2016

PLoS ONE

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Haemonchus contortus is a parasitic gastrointestinal nematode, and its excretory and secretory products (HcESPs) interact extensively with the host cells. In this study, we report the interaction of proteins from HcESPs at different developmental stages to goat peripheral blood mononuclear cells (PBMCs) in vivo using liquid chromatography-tandem mass spectrometry. A total of 407 HcESPs that interacted with goat PBMCs at different time points were identified from a H. contortus protein database using SEQUEST searches. The L4 and L5 stages of H. contortus represented a higher proportion of the identified proteins compared with the early and late adult stages. Both stage-specific interacting proteins and proteins that were common to multiple stages were identified. Forty-seven interacting proteins were shared among all stages. The gene ontology (GO) distributions of the identified goat PBMC-interacting proteins were nearly identical among all developmental stages, with high representation of binding and catalytic activity. Cellular, metabolic and single-organism processes were also annotated as major biological processes, but interestingly, more proteins were annotated as localization processes at the L5 stage than at the L4 and adult stages. Based on the clustering of homologous proteins, we improved the functional annotations of un-annotated proteins identified at different developmental stages. Some unnamed H. contortus ATP-binding cassette proteins, including ADP-ribosylation factor and P-glycoprotein-9, were identified by STRING protein clustering analysis.

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“…Previously, various STPs from number of parasites have been identified and characterized, including those of Oesophagostomum dentatum, Trichostrongylus vitrinus, Toxoplasma gondii, Toxocara canis , and Plasmodium falciparum ( 13 – 17 ), and also suggested that STP-1 from H. contortus play a key role in some biological processes, such as spermatogenesis ( 18 ). However, information regarding the functions of STPs in parasitic nematode H. contortus is scant.…”

Section: Introductionmentioning

confidence: 99%

The Serine/Threonine-Protein Phosphatase 1 From Haemonchus contortus Is Actively Involved in Suppressive Regulatory Roles on Immune Functions of Goat Peripheral Blood Mononuclear Cells

et al. 2018

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Serine/threonine-protein phosphatases (STPs), as integral constituents of parasitic excretory/secretory proteins, are assumed to be released during the host–parasite interactions. However, knowledge about these phosphatases and their immunoregulatory and immune protective efficiencies with host peripheral blood mononuclear cells (PBMCs) is scant. In this study, an open reading frame of STP from Haemonchus contortus designated as HcSTP-1 was amplified and cloned using reverse-transcription-polymerase chain reaction (RT-PCR) method. The 951-bp nucleotides sequence was encoded to a protein of 316 amino acid residues, conserved in characteristics motifs GDXHG, GDYVDRG, GNHE, HGG, RG, and H. The HcSTP-1 protein was detected at approximately 35 kDa as recombinant protein fused in an expression vector system and resolved on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunohistochemically, HcSTP-1 was found to be localized in both male and female adult worm sections. Using immunofluorescence assay, the binding activity of rHcSTP-1 was confirmed on surface of goat PBMCs, which resulted in expression of multiple cytokines and various immunoregulatory activities in vitro. The RT-PCR results showed that mRNA level of interleukin-2, TGF-β1, IFN-γ, and IL-17 (with 10 µg/ml) was upregulated and IL-10 was decreased. However, IL-6 showed no change after PBMCs incubated with rHcSTP-1 protein. Further functional analysis showed that migratory activity of cells, intracellular nitrite production (NO), and apoptotic efficiency of PBMCs were elevated at significant level, whereas the proliferation of goat PBMCs and monocytes-associated major histocompatibility complex (MHC)-I and MHC-II expressions were decreased significantly at concentration-dependent fashion. Our results showed that the HcSTP-1 protein engaged in vital suppressive regulatory roles on host immune cells, which might represent a potential molecular target for controlling H. contortus infection in future.

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Molecular characterization and functional analysis of serine/threonine protein phosphatase of Toxocara canis

Cited by 9 publications

References 39 publications

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles

The Serine/Threonine-Protein Phosphatase 1 From Haemonchus contortus Is Actively Involved in Suppressive Regulatory Roles on Immune Functions of Goat Peripheral Blood Mononuclear Cells

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