Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Li, Fajun; Cui, Xiaocui; Mao, Meina; Fu, Chunpeng; Li, Zongzhen; Liu, Jie; Wang, Aili

doi:10.1016/j.aqrep.2024.102190

Aquaculture Reports

2024

DOI: 10.1016/j.aqrep.2024.102190

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Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Fajun Li,

Xiaocui Cui,

Meina Mao

et al.

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2024

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A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

Cai,

Zhang,

Jiang

et al. 2024

Sci Rep

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Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized the role of the Mn-DHCR24 gene in the oriental river prawn ( Macrobrachium nipponense ). We used bioinformatics to analyze sequence features and phylogenetic relationships of a single Mn-DHCR24 gene. The expression patterns of Mn-DHCR24 across different tissues and developmental stages were determined by real-time PCR, and its localization in testis was determined by in situ hybridization. RNA interference was used to knock down Mn-DHCR24 expression, followed by examining changes in sex ratio and gonadal development at the PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, and tissue sections were used to characterize gonadal development. The results indicated that Mn-DHCR24 was high expression in testis, which was critical for sperm maturation and gonadal differentiation. RNAi experiments showed the role of Mn-DHCR24 during reproductive regulation rather than as a master gene for sex differentiation. This study further showed that Mn-DHCR24 regulated sex and hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into the genetic and hormonal mechanisms of gonadal differentiation in M. nipponense , and supported the development of monosex culture technology.

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A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

Cai,

Zhang,

Jiang

et al. 2024

Sci Rep

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show abstract

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Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Cited by 1 publication

References 39 publications

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

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