Molecular characterization and gene evolution of the heat shock protein 70 gene in snakehead fish with different tolerances to temperature

Eid, Ihlam Ibrahim; Bhassu, Subha; Goh, Zee Hong; Khoo, Li Teng; Tan, Geok Yuan Annie

doi:10.1016/j.bse.2016.02.011

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…HSP is a protein that will respond to external stressful conditions [44]. It protects cells from extreme physiological, pathological, and environmental conditions, and plays a role in protein misfolding correction, preserving immature polypeptides from aggregation under stress [45,46]. Cortisol is the main glucocorticoid hormone in teleosts that are involved in the regulation of metabolic adjustments.…”

Section: Pre-experiment: Selection Of Tiger Grouper Transport Temperamentioning

confidence: 99%

Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Wang

Cao

et al. 2020

Biology

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Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.

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Section: Pre-experiment: Selection Of Tiger Grouper Transport Temperamentioning

confidence: 99%

Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Wang

Cao

et al. 2020

Biology

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“…This may be because fish obtain a better energy distribution in response to stress via growth suppression. Heat shock protein 70 (HSP70), a main molecular chaperone, combines with unfolded or misfolded proteins and also promotes the degradation and removal of denatured proteins [48].…”

Section: Response Regulation Under Salinity and Cold Effectmentioning

confidence: 99%

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

Wang

Tang

et al. 2020

BMC Genomics

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Background: Coilia nasus (C. nasus) is an important anadromous fish species that resides in the Yangtze River of China, and has high ecological and economical value. However, wild resources have suffered from a serious reduction in population, attributed to the over-construction of water conservancy projects, overfishing, and environmental pollution. The Ministry of Agriculture and Rural Affairs of the People's Republic of China has issued a notice banning the commercial fishing of wild C. nasus in the Yangtze River. Wild C. nasus populations urgently need to recover. A better understanding of C. nasus migration patterns is necessary to maximize the efficiency of conservation efforts. Juvenile C. nasus experience a simultaneous effect of increasing salinity and cold stress during seaward migration, and the brain plays a comprehensive regulatory role during this process. Therefore, to explore the early seaward migration regulation mechanism of juvenile C. nasus, we performed a comparative transcriptome analysis on the brain of juvenile C. nasus under salinity and cold stress simultaneously. Results: Relevant neurotransmitters, receptors, and regulatory proteins from three categories of regulatory pathway play synergistic regulatory roles during the migration process: neuronal signaling, the sensory system, and environmental adaptation. The significant differential expression of growth-related hormones, thyroid receptors, haptoglobin, and prolactin receptors was similar to the results of relevant research on salmonids and steelhead trout. Conclusions: This study revealed a regulatory network that the brain of juvenile C. nasus constructs during migration, thereby providing basic knowledge on further studies could build on. This study also revealed key regulatory genes similar to salmonids and steelhead trout, thus, this study will lay a theoretical foundation for further study on migration regulation mechanism of anadromous fish species.

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“…HSP70, HSP90 and metallothionein (MT‐1 and MT‐2) transcription in heart tissues variably changed over time following cold shock (12 to 5°C) in common carp, with all gene transcription levels increasing to some degree except MT‐1 which decreased over 1–2 h of exposure (Ali et al ., 2010). HSP70 transcription was significantly upregulated in the kidneys, muscles and fins of striped snakehead ( Channa striata Bloch 1793) cold shocked (28 to 16°C) for 1 h relative to transcription levels in the liver (which were treated as a baseline) and stomach and gill tissues (which were slightly upregulated; Eid et al ., 2016). HSP90 transcription was upregulated in the liver, spleen, brain and intestine, but not kidney, in cold‐shocked grass carp (4°C for 2 h from an unspecified starting temperature; Wu et al ., 2012).…”

Section: Cold Stress and The Generalized Stress Response In Fishmentioning

confidence: 99%

An updated review of cold shock and cold stress in fish

Reid

Patrick²,

Rytwinski

et al. 2022

Journal of Fish Biology

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Temperature is critical in regulating virtually all biological functions in fish. Low temperature stress (cold shock/stress) is an often-overlooked challenge that many fish face as a result of both natural events and anthropogenic activities. In this study, we present an updated review of the cold shock literature based on a comprehensive literature search, following an initial review on the subject by M.R. Donaldson and colleagues, published in a 2008 volume of this journal. We focus on how knowledge on cold shock and fish has evolved over the past decade, describing advances in the understanding of the generalized stress response in fish under cold stress, what metrics may be used to quantify cold stress and what knowledge gaps remain to be addressed in future research. We also describe the relevance of cold shock as it pertains to environmental managers, policymakers and industry professionals, including practical applications of cold shock. Although substantial progress has been made in addressing some of the knowledge gaps identified a decade ago, other topics (e.g., population-level effects and interactions between primary, secondary and tertiary stress responses) have received little or no attention despite their significance to fish biology and thermal stress. Approaches using combinations of primary, secondary and tertiary stress responses are crucial as a research priority to better understand the mechanisms underlying cold shock responses, from short-term physiological changes to individual-and population-level effects, thereby providing researchers with better means of quantifying cold shock in laboratory and field settings.

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Molecular characterization and gene evolution of the heat shock protein 70 gene in snakehead fish with different tolerances to temperature

Cited by 11 publications

References 19 publications

Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

An updated review of cold shock and cold stress in fish

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