Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Ritdachyeng, Eakartit; Manaboon, Manaporn; Tobe, Stephen S.; Singtripop, Tippawan

doi:10.1016/j.jinsphys.2012.09.002

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…fuscidentalis has not been determined. A previous study has shown that the expression level of juvenile hormone binding protein (JHBP) encoding gene in the fat body was high in the diapause period and low from the late-diapause until pupation [75]. Change in the JH titer in the hemolymph toward diapause termination is still to be determined.…”

Section: Discussionmentioning

confidence: 99%

Larval diapause termination in the bamboo borer, Omphisa fuscidentalis

et al. 2017

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In insects, juvenile hormone (JH) and 20-hydroxyecdysone (20E) regulate larval growth and molting. However, little is known about how this cooperative control is terminating larval diapause especially in the bamboo borer, Omphisa fuscidentalis. In both in vivo and in vitro experiments, we here measured the expression levels of genes which were affected by juvenile hormone analogue (JHA: S-methoprene) and 20-hydroxyecdysone (20E) in diapausing O. fuscidentalis larvae. Corresponding mRNA expression changes in the subesophageal ganglion (SG) and prothoracic gland (PG) were evaluated using qRT-PCR. The data showed similar response patterns of JH receptor gene (OfMet), diapause hormone gene (OfDH-PBAN), ecdysone receptor genes (OfEcR-A and OfEcR-B1) and ecdysone inducible genes (OfBr-C, OfE75A, OfE75B, OfE75C and OfHR3). JHA induced the expressions of OfMet and OfDH-PBAN in both SG and PG, whereas ecdysone receptor genes and ecdysone inducible genes were induced by JHA only in PG. For 20E treatment group, expressions of ecdysone receptor genes and ecdysone inducible genes in both SG and PG were increased by 20E injection. In addition, the in vitro experiments showed that OfMet and OfDH-PBAN were up-regulated by JHA alone, but ecdysone receptor genes and ecdysone inducible genes were up-regulated by JHA and 20E. However, OfMet and OfDH-PBAN in the SG was expressed faster than OfMet and OfDH-PBAN in the PG and the expression of ecdysone receptor genes and ecdysone inducible genes induced by JHA was much later than observed for 20E. These results indicate that JHA might stimulate the PG indirectly via factors (OfMet and OfDH-PBAN) in the SG, which might be a regulatory mechanism for larval diapause termination in O. fuscidentalis.

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Section: Discussionmentioning

confidence: 99%

Larval diapause termination in the bamboo borer, Omphisa fuscidentalis

et al. 2017

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“…It is possible that JHBP mRNA levels correlate with JH titre during the development of O. fuscidentalis (Ritdachyeng et al ., 2012). Moreover, the changes in the cuticle and expression of OfJHBP in the integument after JHA application are evidence for the integument comprising a JH-target organ.…”

Section: Discussionmentioning

confidence: 99%

“…The gene-specific primers used for amplifying cDNA fragments corresponding to OfJHBP (GenBank accession number JQ658990) were: forward primer (5-TTC AAA ATG GAC AGA GAG GCG-3 ) and reverse primer (5 -CTC ACA CGT TAT AGT CTC AGG-3 ) and, for endogenous control, OfRpL3 (O. fuscidentalis ribosomal protein L3, accession number EF453378), forward primer (5 -TCT ACC CCA AGA AGA GGT CTC G-3 ) and reverse primer (5 -ACG ACA GTC CTC AGA CAT GTG C-3 ). These primers were based on the respective sequences of the cloned fulllength OfJHBP and OfRpL3 (Ritdachyeng et al ., 2012). The THUNDERBIRD SYBR qPCR Mix (Toyobo, Japan) was used to perform qRT-PCR in an iCycler iQ5 Real-time PCR Detection System (Bio-Rad, Hercules, California).…”

Section: Qrt-pcrmentioning

confidence: 99%

“…Ritdachyeng et al . () report the characterization and cloning of the gene encoding JHBP from O. fuscidentalis ( OfJHBP , GenBank accession number JQ658990). The expression patterns of OfJHBP in various tissues reveal the target organs of JH.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, JHBP is found in cytosol of larval and pupal epidermis of Galleria mellonella (Wiśniewski et al ., 1988) and a JHBP with receptor properties has been detected in the epidermis of the Dipteran Drosophila hydei (Klages et al ., 1980(Klages et al ., , 1981. Ritdachyeng et al . (2012) report the characterization and cloning of the gene encoding JHBP from O. fuscidentalis (OfJHBP , GenBank accession number JQ658990).…”

Section: Introductionmentioning

confidence: 99%

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Possible roles of Juvenile Hormone and Juvenile Hormone binding protein on changes in the integument during termination of larval diapause in the bamboo borer Omphisa fuscidentalis

et al. 2013

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To understand the role of Juvenile Hormone (JH) on changes in the integument of Omphisa fuscidentalis Hampson (Lepidoptera: Pyralidae) during larval–pupal development, the expression patterns of transcripts of the O. fuscidentalis JH binding protein gene (OfJHBP) are determined in the integument of diapausing larvae. Quantitative real‐time polymerase chain reaction (qRT‐PCR) studies show that the relative levels OfJHBP mRNA in the integument do not differ significantly in different segments of the body. Application of the JH analogue (JHA) methoprene to diapausing larvae results in a relatively low expression of OfJHBP mRNA from days 0 to 8 after JHA application and reaches maximal levels in the pupal stage. Changes in OfJHBP expression after 20‐hydroxyecdysone (20E) injection also show low levels of OfJHBP mRNA from days 0 to 8 and show a peak of expression in the pupal stage. Incubation of the integument in vitro in the presence of JHA (3.22 µm) induces high levels of OfJHBP expression within 120 min, whereas incubation with 20E (2.08 µm) induces gene expression at 150 min. To study the cooperative effect on OfJHBP expression of these two hormones, dose–response experiments are performed. Larval integument is maintained in the presence of 0.32 µm JHA or 2.08 µm 20E or a combination of both hormones in vitro for 120 min. Induction of OfJHBP expression by JHA and 20E in combination is significantly higher than that of either hormone alone. The results impact not only on our understanding of how methoprene (and hence JH) terminates larval diapause through an increase in ecdysteroid titre in the haemolymph, but also on how JH acts directly on the integument to stimulate the expression of a JH binding protein gene.

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Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

Suang

Hiruma

Kaneko

et al. 2019

Arch Insect Biochem Physiol

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Larval diapause in many lepidopteran insects is induced and maintained by high juvenile hormone (JH). In the case of the bamboo borer, Omphisa fuscidentalis, the effect of JH is the opposite: The application of juvenile hormone analog (JHA: S‐methoprene) terminates larval diapause, unlike in other insect species. Here, we analyzed the expression of JH‐receptor Met, DH‐PBAN, and Kr‐h1 in the subesophageal ganglion (SG) from October to April using semi‐quantitative polymerase chain reaction (PCR). The results show that OfMet and OfDH‐PBAN messenger RNA in the SG are mainly expressed during the larval diapause stage, while OfKr‐h1 increases during the pupal stage. Using tissue culture techniques and an enzyme‐linked immunosorbent assay (ELISA), diapause hormone (DH) was found to induce ecdysteroidogenesis in the culture medium of the prothoracic gland (PG) after incubation for 30 min with 25 ng and 50 ng of DH. Thus, DH is a novel stimulator for the PG. We identified a DHR homolog in the bamboo borer and confirmed that it is expressed in the PG. In addition, for in vitro experiments, DH increased the expression levels of OfDHR, OfEcR‐A, and ecdysone‐inducible genes in the PG. These results demonstrate that DH can function as a prothoracicotropic factor, and this function of DH might be through of DHR expressed on PG cells. Consequently, DH is one of the key factors in larval diapause break which is triggered by JH in the bamboo borer, O. fuscidentalis.

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Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Cited by 21 publications

References 26 publications

Larval diapause termination in the bamboo borer, Omphisa fuscidentalis

Larval diapause termination in the bamboo borer, Omphisa fuscidentalis

Possible roles of Juvenile Hormone and Juvenile Hormone binding protein on changes in the integument during termination of larval diapause in the bamboo borer Omphisa fuscidentalis

Diapause hormone directly stimulates the prothoracic glands of diapause larvae under juvenile hormone regulation in the bamboo borer, Omphisa fuscidentalis Hampson

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