Molecular characterization and genomic mapping of the pathogenesis-related protein 1 (PR-1) gene family in hexaploid wheat (Triticum aestivum L.)

Lu, Shunwen; Friesen, Timothy L.; Faris, Justin D.

doi:10.1007/s00438-011-0618-z

Cited by 62 publications

(69 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are 23 TaPR‐1‐type genes in wheat identified thus far and these are divided into three major groups, based on their theoretical isoelectric point (Lu et al ., ). As described above, SnTox3 interacts with the basic group I proteins TaPR‐1‐1 and TaPR‐1‐5.…”

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Wheat PR‐1 proteins are targeted by necrotrophic pathogen effector proteins

et al. 2016

View full text Add to dashboard Cite

Recent studies have identified that proteinaceous effectors secreted by Parastagonospora nodorum are required to cause disease on wheat. These effectors interact in a gene-for-gene manner with host-dominant susceptibilty loci, resulting in disease. However, whilst the requirement of these effectors for infection is clear, their mechanisms of action remain poorly understood. A yeast-two-hybrid library approach was used to search for wheat proteins that interacted with the necrotrophic effector SnTox3. Using this strategy we indentified an interaction between SnTox3 and the wheat pathogenicity-related protein TaPR-1-1, and confirmed it by in-planta co-immunprecipitation. PR-1 proteins represent a large family (23 in wheat) of proteins that are upregulated early in the defence response; however, their function remains ellusive. Interestingly, the P. nodorum effector SnToxA has recently been shown to interact specifically with TaPR-1-5. Our analysis of the SnTox3-TaPR-1 interaction demonstrated that SnTox3 can interact with a broader range of TaPR-1 proteins. Based on these data we utilised homology modeling to predict, and validate, regions on TaPR-1 proteins that are likely to be involved in the SnTox3 interaction. Precipitating from this work, we identified that a PR-1-derived defence signalling peptide from the C-terminus of TaPR-1-1, known as CAPE1, enhanced the infection of wheat by P. nodorum in an SnTox3-dependent manner, but played no role in ToxA-mediated disease. Collectively, our data suggest that P. nodorum has evolved unique effectors that target a common host-protein involved in host defence, albeit with different mechanisms and potentially outcomes.

show abstract

Section: Resultsmentioning

confidence: 97%

“…A detailed genomic mapping study by Lu et al . () identified 23 TaPR‐1 genes in wheat. Based on theoretical isoelectric points, these 23 genes encode seven acidic and 16 basic TaPR‐1 proteins.…”

Section: Discussionmentioning

confidence: 99%

Wheat PR‐1 proteins are targeted by necrotrophic pathogen effector proteins

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The genomic and coding sequences of the wheat ACT1 gene (GenBank accession numbers KC775780 and KC775781, respectively) were amplified by PCR/reverse transcriptase PCR as described previously50 and the PCR products were cloned into a pCR2.1 vector using the TOPO TA Cloning Kit (Invitrogen) and sequenced at Cornell University Biotechnology Resource Center. Double-stranded DNA fragments were amplified by PCR using primers given in Supplementary Table S3 and PCR products were purified using the QIAquick PCR Purification Kit (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

Zn2+ Blocks Annealing of Complementary Single-Stranded DNA in a Sequence-Selective Manner

2014

Sci Rep

Self Cite

View full text Add to dashboard Cite

Zinc is the second most abundant trace element essential for all living organisms. In human body, 30–40% of the total zinc ion (Zn2+) is localized in the nucleus. Intranuclear free Zn2+ sparks caused by reactive oxygen species have been observed in eukaryotic cells, but question if these free Zn2+ outrages could have affected annealing of complementary single-stranded (ss) DNA, a crucial step in DNA synthesis, repair and recombination, has never been raised. Here the author reports that Zn2+ blocks annealing of complementary ssDNA in a sequence-selective manner under near-physiological conditions as demonstrated in vitro using a low-temperature EDTA-free agarose gel electrophoresis (LTEAGE) procedure. Specifically, it is shown that Zn2+ does not block annealing of repetitive DNA sequences lacking CG/GC sites that are the major components of junk DNA. It is also demonstrated that Zn2+ blocks end-joining of double-stranded (ds) DNA fragments with 3′ overhangs mimicking double-strand breaks, and prevents renaturation of long stretches (>1 kb) of denatured dsDNA, in which Zn2+-tolerant intronic DNA provides annealing protection on otherwise Zn2+-sensitive coding DNA. These findings raise a challenging hypothesis that Zn2+-ssDNA interaction might be among natural forces driving eukaryotic genomes to maintain the Zn2+-tolerant repetitive DNA for adapting to the Zn2+-rich nucleus.

show abstract

“…The fundamental frequency (f0) and the glottal-to-noise excitation ratio (GNE) were the measures considered for this analysis [6]. …”

Section: Methodsmentioning

confidence: 99%

Voice analysis after cancer treatment with organ preservation

et al. 2011

View full text Add to dashboard Cite

BackgroundThis cross-sectional study objects to measure, subjectively and objectively, the voice and life quality of patients with oral cavity, pharyngeal and laryngeal cancer, after organ-preservation treatment.Methods25 cases diagnosed and treated at a high complexity oncology center in southeastern Brazil. All had oral cavity, pharyngeal or laryngeal cancer, with a therapeutic proposal of radiotherapy alone or simultaneous radiochemotherapy. Acoustic voice analysis and the Voice Handicap Index protocol were used to measure voice quality. The data were analyzed through the χ2, Student's t and Kruskal Wallis tests. Significance level was 5%.ResultsAfter treatment, 40% complained of hoarseness, 56% complained of throat clearing, and no patient reported aphonia. On the voice quality auditory scale, 36% had moderate dysphonia. Acoustic voice analysis ranged from 184 to 221 Hz in females, and from 92 to 241 Hz in males. As for quality of life, most patients had mild physical, functional and emotional handicaps.ConclusionsChemio-radiation organ preservation protocols in the patients studied may leave the organ but with reduced function which brings communication sequelae. In such cases, voice assessment and quality of life protocols, as well as speech therapy rehabilitation, are important tools to preserve function, measure and treat alterations, and reintegrate patients into the community.

show abstract

Molecular characterization and genomic mapping of the pathogenesis-related protein 1 (PR-1) gene family in hexaploid wheat (Triticum aestivum L.)

Cited by 62 publications

References 74 publications

Wheat PR‐1 proteins are targeted by necrotrophic pathogen effector proteins

Wheat PR‐1 proteins are targeted by necrotrophic pathogen effector proteins

Zn2+ Blocks Annealing of Complementary Single-Stranded DNA in a Sequence-Selective Manner

Voice analysis after cancer treatment with organ preservation

Contact Info

Product

Resources

About