Molecular characterization and hematological analysis of Listeria monocytogenes infection in dairy cows in Punjab (Pakistan)

Munir, Muhammad Zubair; Khan, Jawaria Ali; Ijaz, Muhammad; Akhtar, Fareeha

doi:10.1007/s00203-022-02804-1

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…Pakistan and especially Punjab, has inadequate information about the risks of cow's milk. According to this study, 3.43% of cow's milk contains L. monocytogenes (Munir et al 2022). Over the years, there have been several listeriosis outbreaks reported globally.…”

Section: Overview Of Listeriosis Prevalence Worldwidementioning

confidence: 85%

Global Prevalence of Listeriosis

2023

IJAB

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Listeria monocytogenes, a Gram-positive bacterium, is the causative agent of listeriosis, a severe foodborne infection affecting both animals and humans. Despite being initially recognized as a rare disorder, recent outbreaks in various regions have brought attention to its significant impact on public health. This zoonotic pathogen, categorized as a rare condition by ORPHANET, has become the sixth most prevalent zoonotic disease in Europe, with high mortality rates. Listeriosis cases have been on the rise in Europe since 2008, posing a substantial threat to vulnerable populations. The adaptability of L. monocytogenes to diverse environmental conditions, including food processing and agricultural areas, highlights its ability to persist and spread. Virulence factors, bacterial strain characteristics, and host susceptibility contribute to the severity of listeriosis. Insufficient epidemiological evidence hampers the estimation of contamination severity in most outbreaks. Government regulatory agencies enforce strict guidelines and programs in the food industry to control L. monocytogenes spread. Listeriosis primarily spreads through contaminated food, with infective dosages varying based on health conditions. Highrisk groups, such as the elderly and immunocompromised individuals, are advised to avoid consuming high-concentration L. monocytogenes foods. The disease's protracted incubation period, propensity for severe clinical signs, and challenges in controlling its spread contribute to its severity. Global prevalence, outbreaks, and risk factors underscore the need for effective control measures. L. monocytogenes can contaminate various food sources, emphasizing the importance of stringent food safety practices. Laboratory data, microbiological diagnosis, and health risks associated with listeriosis further contribute to understanding and managing this public health threat.

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Section: Overview Of Listeriosis Prevalence Worldwidementioning

confidence: 85%

Global Prevalence of Listeriosis

2023

IJAB

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“…L. monocytogenes can cause clinical Listeriosis in encephalitis, abortion and septicaemia in humans (Sanaa et al, 1993;Savran et al, 2018). Dairy products including processed and raw milk have been strongly implicated in prevalent of Listeriosis (Schmidt et al, 2009;Munir et al, 2022;Camargo et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial mechanism of garviecin LG34 against S. aureus and L. monocytogenes and its application in milk preservation.

Gao

2024

Preprint

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Purpose The objective of this study was to reveal the antibacterial mode of action of garviecin LG34 against S. aureus CICC 21600 and L. monocytogenes CICC 21633 and measure the inhibitions on these two foodborne pathogenic bacteria in milk. Methods Antibacterial mechanism of garviecin LG34 was ascertained by its effect on the efflux of Potassium (K+) ions, extracellular electrical conductivity, UV-absorbing substances, potential across the membrane (ΔΨ) and cell permeability. The inhibitions of garviecin LG34 against S. aureus CICC 21600 and L. monocytogenes CICC 21600 in milk was studied by viable counting method. Results Supplementation with 160 AU/ml of garviecin LG34 had a bactericidal effect on S. aureus CICC 21600 and L. monocytogenes CICC 21633. 160 AU/ml of garviecin LG34 resulted in the effusion of potassium ion and UV-absorbing substances, the leakage of cellular electrolytes and the dissipation of electrical potential across the membrane of these two food-borne bacteria. Moreover, the increase in cell permeability of both strains were observed by flow cytometer after cells treated with 160 AU/ml of garviecin LG34. Garviecin LG34 significantly inhibited the growth of these two food-borne bacteria in the dairy products especially in skimmed milk. Conclusion Garviecin LG34 could cause pore formation, intracellular materials release and permeability increase of S. aureus CICC 21600 and L. monocytogenes CICC 21633, and could be applied to milk as bio-preservative.

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