Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans

Lang, Guo-Jun; Zhang, Mingyan; Li, Baoling; Yu, Lie; Lu, Xin-Jiang; Zhang, Chuan‐Xi

doi:10.5483/bmbrep.2010.43.8.573

Cited by 3 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we propose that ace2 functions upstream of ace1. Furthermore, ace2 is the only AChE gene in D. melanogaster and other Cyclorrhapha (Diptera) (Weill et al, 2002), including the silkworm maggot (Exorista sorbillans Wiedemann), Australian blow fly [Lucilia cuprina (Wiedemann)], olive fruit fly (Bactrocera oleae Gmelin), and the house fly (Musca domestica L.) (Lang et al, 2010). Both ace genes derive from an old gene duplication and apparently, ace1 was secondarily lost in the lineage that lead to the Cyclorrapha (Huchard et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Functional study of acetylcholinesterase genes in Bombyx mori ovary cells using RNA interference

Cao

Liu

Wang

et al. 2012

Entomologia Exp Applicata

View full text Add to dashboard Cite

Acetylcholinesterase (AChE) plays a key role in neurotransmission, in which it maintains the transmission of nerve impulses by catalyzing acetylcholine in the neuronal synapse. Acetylcholinesterase in Lepidoptera is encoded by two ace genes, ace1 and ace2. In this study, we used RNA interference to individually knock down the expression of ace1 or ace2 in a Bombyx mori (L.) (Lepidoptera: Bombycidae) cell line (BmN) to explore their functions and relationships. Knockdown efficiency was confirmed using quantitative real‐time PCR. We also monitored transcription levels of ace1 and ace2 at different time points after B. mori nucleopolyhedrovirus (BmNPV) infection. We found that when the expression of ace1 was knocked down to 37.5%, the expression of ace2 increased by 66%; when ace2 expression was reduced to 33.4%, the ace1 expression was similarly reduced to 26.7%. Acetylcholinesterase activity was decreased to 86.2% when ace2 expression was inhibited. However, no significant difference was observed in AChE activity when ace1 expression was knocked down. After BmNPV infection, ace1 expression was gradually increasing, whereas ace2 expression was gradually decreasing. Our study indicates that ace2 plays an important compensatory role for the loss of ace1 in maintaining AChE activity by increasing its expression level. In addition, ace2 may also regulate transcription of ace1. Overall, our findings help elucidate the relationship between the two ace genes in Lepidoptera.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional study of acetylcholinesterase genes in Bombyx mori ovary cells using RNA interference

Cao

Liu

Wang

et al. 2012

Entomologia Exp Applicata

View full text Add to dashboard Cite

show abstract

“…(iv) For density treatment, the larval parasitoids feed on a single host at densities of 1-5 larvae/host, the parasitoids were collected on Day 1 at 3rd instar. (v) For pesticide treatment, the 5th instar B. mori larvae were sprayed with 0.05% dimethoate (Aladdin) (100 μL/host), a commonly used insecticide for management of E. sorbillans in sericulture, at 1 day after E. sorbillans parasitism (Lang et al, 2010). The parasitoids from double distilled H 2 O treated hosts were used as controls.…”

Section: Experimental Set-upmentioning

confidence: 99%

Evaluation of suitable reference genes for expression analysis using quantitative real‐time polymerase chain reaction in the parasitoid Exorista sorbillans (Diptera: Tachinidae)

Yang

Zhe

et al. 2023

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The parasitoid Exorista sorbillans (Diptera: Tachinidae) is a larval endoparasitoid of the silkworm Bombyx mori, causing severe damage to silkworm cocoon industry. It is also an important natural enemy resource of insect pests in agriculture and forestry. Despite their roles in biocontrol and pest status on sericulture, there has been limited research on the functional studies of dipteran parasitoids.Quantitative real-time polymerase chain reaction (qRT-PCR) is the most commonly used to address gene functions.Using qRT-PCR, stably expressed reference genes under different experimental conditions are required to normalize the expression of target genes. However, no information regarding suitable qRT-PCR reference genes in dipteran parasitoids has been reported. In this study, we evaluate the expression stability of nine commonly used reference genes in insects including eukaryotic translation elongation factor 1δ (eEF1δ), elongation factor 2, 18S ribosomal RNA (18S rRNA), tubulin 3, actin87, ribosomal protein 49 (RP49), ribosomal protein S15, glyceraldehyde-3-phosphate dehydrogenase, and TATA-box binding protein (TBP) in E. sorbillans under different treatments, including tissues, developmental stages, genders, feeding density and pesticide stress, using ΔC t , BestKeeper, geNorm, Normfinder

show abstract

Cloning and Characterization of the Acetylcholinesterase1 Gene of Tetranychus cinnabarinus (Acari: Tetranychidae)

Feng

Wang

et al. 2015

Journal of Economic Entomology

View full text Add to dashboard Cite

The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a major agriculture pest. It can be found worldwide, has an extensive host plant range, and has shown resistance to pesticides. Organophosphate and carbamate insecticides account for more than one-third of all insecticide sales. Insecticide resistance and the toxicity of organophosphate and carbamate insecticides to mammals have become a growing concern. Acetylcholinesterase (AChE) is the major targeted enzyme of organophosphate and carbamate insecticides. In this study, we fully cloned, sequenced and characterized the ace1 gene of T. cinnabarinus, and identified the differences between T. cinnabarinus AChE1, Tetranychus urticae Koch AChE1, and human AChE1. Resistance-associated target-site mutations were displayed by comparing the AChE amino acid sequences and their AChE three-dimensional (3D) structures of the insecticide-susceptible strains of T. cinnabarinus and T. urticae to that of a T. urticae-resistant strain. We identified variation in the active-site gorge and the sites interacting with gorge residues by comparing AChE1 3D structures of T. cinnabarinus, T. urticae, and humans, though their 3D structures were similar. Furthermore, the expression profile of T. cinnabarinus AChE, at the different developmental stages, was determined by quantitative real-time polymerase chain reaction; the transcript levels of AChE were higher in the larvae stage than in other stages. The changes in AChE expression between different developmental stages may be related to their growth habits and metabolism characteristics. This study may offer new insights into the problems of insecticide resistance and insecticide toxicity of nontarget species.

show abstract

Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans

Cited by 3 publications

References 15 publications

Functional study of acetylcholinesterase genes in Bombyx mori ovary cells using RNA interference

Functional study of acetylcholinesterase genes in Bombyx mori ovary cells using RNA interference

Evaluation of suitable reference genes for expression analysis using quantitative real‐time polymerase chain reaction in the parasitoid Exorista sorbillans (Diptera: Tachinidae)

Cloning and Characterization of the Acetylcholinesterase1 Gene of Tetranychus cinnabarinus (Acari: Tetranychidae)

Contact Info

Product

Resources

About