Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin

Yan, Wen; Li, Guoqing; Jiao, Xiaoyu; Cheng, Xi; Abdullah, Muhammad; Li, Dahui; Lin, Yi; Cai, Yongping; Nie, Fan

doi:10.1242/bio.036483

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…Genetic modifications of fungal strains in order to improve lignin degradation, restrict carbohydrates loss, raise the growth rate, or increase the optimal growth temperature have the potential to improve the fungal pretreatment process in the future. White-rot fungi have been modified for overexpression of ligninolytic enzymes [106], heterologous expression of lignocellulases [107,108], and avoidance of cellulase production to restrict cellulose loss [32]. Developing new microbial strains that exploit the ligninolytic systems of white-rot fungi, while including benefits of different microorganisms, such as the higher growth rate of bacteria or yeast, could help develop a better platform for the optimization of biological pretreatment.…”

Section: Potential Strategies To Reduce the Sugar Production Costmentioning

confidence: 99%

Techno-Economic Bottlenecks of the Fungal Pretreatment of Lignocellulosic Biomass

Vasco‐Correa

Shah

2019

Fermentation

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Fungal pretreatment is a biological process that uses rotting fungi to reduce the recalcitrance and enhance the enzymatic digestibility of lignocellulosic feedstocks at low temperature, without added chemicals and wastewater generation. Thus, it has been presumed to be low cost. However, fungal pretreatment requires longer incubation times and generates lower yields than traditional pretreatments. Thus, this study assesses the techno-economic feasibility of a fungal pretreatment facility for the production of fermentable sugars for a 75,700 m 3 (20 million gallons) per year cellulosic bioethanol plant. Four feedstocks were evaluated: perennial grasses, corn stover, agricultural residues other than corn stover, and hardwood. The lowest estimated sugars production cost ($1.6/kg) was obtained from corn stover, and was 4-15 times as much as previous estimates for conventional pretreatment technologies. The facility-related cost was the major contributor (46-51%) to the sugar production cost, mainly because of the requirement of large equipment in high quantities, due to process bottlenecks such as low sugar yields, low feedstock bulk density, long fungal pretreatment times, and sterilization requirements. At the current state of the technology, fungal pretreatment at biorefinery scale does not appear to be economically feasible, and considerable process improvements are still required to achieve product cost targets.Fermentation 2019, 5, 30 2 of 23 of water [5]. Additionally, most commercially available pretreatments, such as dilute acid and liquid hot water pretreatment, require an additional detoxification step, because their severe conditions promote the generation of compounds derived from lignocellulose, such as furans, weak acids and aromatic compounds, which could inhibit fermentative microorganisms, such as yeast and bacteria [6].A series of alternative methods have been proposed to reduce the severity of the conditions required for biomass fractionation and pretreatment. Use of extrusion, microwaves, ultrasound, or pulse electric field has shown to improve the biomass pretreatment process and allowed for the use of milder pretreatment conditions [7]. Biomass pretreatment with aqueous organic solvents (organosolv) [8,9], ozone (ozonolysis) [10], ionic liquids [11,12], or deep eutectic solvents [13] has shown promising results in biomass fractionation [14,15]. However, concerns about the costs, handling, and recovery of the chemicals present limitations in their large-scale applicability [14,16].Fungal pretreatment is as an alternative to conventional pretreatments that is performed at 25-30 • C, atmospheric pressure, in solid-state with low water use, and without added chemicals that can be corrosive and would need disposal or recovery [17]. Fungal pretreatment is generally performed using wood-rotting fungi, such as white-, brown-, or soft-rot fungi, due to their ability to modify the components of the lignocellulosic biomass. Brown-rot fungi mainly degrade cellulose and hemicellulose, with little modificati...

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Section: Potential Strategies To Reduce the Sugar Production Costmentioning

confidence: 99%

Techno-Economic Bottlenecks of the Fungal Pretreatment of Lignocellulosic Biomass

Vasco‐Correa

Shah

2019

Fermentation

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“…Pleurotus spp. are thought to degrade lignin with laccases (LaCs) and manganese dependent peroxidases (MnPs) that they produce ( Knop et al, 2014 ; Xiao et al, 2017 ; Wang et al, 2019b ). However, whether they can produce lignin peroxidases (LiPs) is debatable and has yet to be proven ( Asgher et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Ligninolytic characteristics of Pleurotus ostreatus cultivated in cotton stalk media

Wang

et al. 2022

Front. Microbiol.

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Biodelignification is widely regarded as a low-efficiency process because it is usually slow and difficult to control. To improve its efficiency and understand its mechanism, the present study analyzed the delignification characteristics of Pleurotus ostreatus grown on a cotton stalk medium. The results demonstrated that all strains of P. ostreatus can selectively degrade the cotton stalk lignin. When cultured in a cotton stalk medium for 60 days, P. ostreatus degraded lignin primarily during its mycelium growth with up to 54.04% lignin degradation and produced laccase and manganese dependent peroxidase with high activity levels at the peaks of 70.17 U/ml and 62.39 U/ml, respectively, but no detectable lignin peroxidase. The results of nuclear magnetic resonance spectroscopy and Fourier transform infrared spectroscopy analyses of significant changes in lignin structure revealed that syringyl (S) lignin units were more degraded than guaiacyl (G) lignin units, with a significantly elevated G/S ratio. The Gas Chromatography-Mass Spectrometer analysis of low-molecular-weight compounds revealed that the delignification resulted in the formation of alcohols, organic acids, benzodiazepines, and alkanes. Identified benzodiazepines implied the degradation of G and S units of lignin. These findings will help to improve the efficiency of biodelignification and expand our understanding of its mechanism.

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“…In contrast, the overexpression of the enzyme manganese peroxidase pomnp6 and versatile peroxidase povp3 in P. ostreatus led to higher lignin degradation in cotton stalks. These data suggested that pomnp6 and povp3 might help develop sustainable energy (Wang et al, 2019). Likewise, the protein kinase A catalytic subunit (PKAc) is essential for inducing the wood degradation system in P. ostreatus.…”

Section: Mutants and Overexpression Of Genesmentioning

confidence: 96%

Overview of Pleurotus spp., edible fungi with various functional properties

Juárez-Hernández,

Pérez-Zavala,

Román-Reyes

et al. 2023

IFRJ

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Pleurotus fungi, also known as basidiomycetous fungi, have been a part of human culture for thousands of years. They exhibit anticancer, antitumor, antibacterial, and immunomodulatory effects, having biotechnological, medicinal, and aesthetic applications. They are also versatile, highly resistant to illnesses and pests, and do not require special growing conditions. These properties make them readily marketable, and can be found in supermarkets worldwide, generating multimillion-dollar sale revenues. The global edible mushroom market was valued at USD 5.08 billion in 2021, which is expected to grow to USD 6.43 billion in 2028. China produces about 87% of Pleurotus spp. globally; other Asian countries generate 12%, and Europe and America account for approximately 1%. Pleurotus spp. have distinct functional characteristics, including high protein content with a proper essential amino acid score pattern, dietary fibre profile, high amounts of vitamins (e.g., B and D) and minerals (e.g., Fe, Zn, Cu, and Se), and low fat. Therefore, Pleurotus spp. can provide alternative industrial tools. The present review discusses Pleurotus spp. as biotechnological tools for acquiring metabolites of interest, studying them, and analysing bioactive substances that can be used in various fields, including medicine and food.

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Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin

Cited by 7 publications

References 40 publications

Techno-Economic Bottlenecks of the Fungal Pretreatment of Lignocellulosic Biomass

Techno-Economic Bottlenecks of the Fungal Pretreatment of Lignocellulosic Biomass

Ligninolytic characteristics of Pleurotus ostreatus cultivated in cotton stalk media

Overview of Pleurotus spp., edible fungi with various functional properties

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