Molecular characterization and phylogenetic analysis of NBS-LRR genes in wild relatives of eggplant (Solanum melongena L

Abstract

Eggplantor brinjal (Solanum melongena L.), is highly susceptible to various soil-borne diseases. The extensive use of chemical fungicides to combat these diseases can be minimized by identification of resistance gene analogs (RGAs) in wild species of cultivated plants.In the present study, degenerate PCR primers for the conserved regions ofnucleotide binding site-leucine rich repeat (NBS-LRR) were used to amplify RGAs from wild relatives of eggplant (Black nightshade (Solanum nigrum), Indian nightshade (Solanumviolaceum)and Solanu mincanum) which showed resistance to the bacterial wilt pathogen, Ralstonia solanacearumin the preliminary investigation. The amino acid sequence of the amplicons when compared to each other and to the amino acid sequences of known RGAs deposited in Gen Bank revealed significant sequence similarity. The phylogenetic analysis indicated that they belonged to the toll interleukin-1 receptors (TIR)-NBS-LRR type R-genes. Multiple sequence alignment with other known R genes showed significant homology with P-loop, Kinase 2 and GLPL domains of NBS-LRR class genes. There has been no report on R genes from these wild eggplants and hence the diversity analysis of these novel RGAs can lead to the identification of other novel R genes within the germplasm of different brinjal plants as well as other species of Solanum.

Documentation of floristic diversity in Ponparakunnu hills, Kozhikode District, Kerala and biological activities of Eclipta prostrata L. and Eupatorium triplinerve Vahl (Asteraceae)

“…Similarly Prakashkumar Unnikrishnan et al, (2014) [27] reported that that the essential oil extracted from the leaves and stem of E. triplinerve is the source of thymohydroquinone dimethyl ether which is found to have significant antimicrobial effect. Sona Dev et al, (2018) [31] reported that Silver nanoparticles (AgNPs) which are bio synthesized from the leaf extracts of E. triplinerve showed excellent antibacterial activity. Akhtar Nahid et al, (2017) [32] interpreted the antimicrobial activity of E. prostrata as the plant extract showed the ability to inhibit the growth of both gram positive and gram negative bacterial strains.…”

​Association of ISSR and ISSR-RGA Markers with Powdery Mildew Resistance Gene in Mungbean

“…It is interesting to note that a large number of R genes, which share several conserved domains i.e., leucine-rich repeat (LRR), nucleotidebinding sites (NBS) and protein kinase domain, implicate resistance against a large spectrum of pathogens. These conserved domains have been found in genes isolated from genomes of several plant species i.e., tomato, eggplant and legume crops (Wei et al, 2016;Dev et al, 2018;Tirnaz et al, 2020). Recently, we developed a new type of marker, ISSRanchored resistance gene analog (ISSR-RGA), using ISSR primers combined with gene-targeted RGA primers to detect the SSRs dispersed at multiple loci in proximity to RGAs throughout the mungbean genome and found that the ISSR-RGA markers are more effective than ISSRs for successfully identifying the gene conferring PM resistance in the 'CN72 × V4718' cross (Poolsawat et al, 2017).…”

Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential

“…The SERK3 was amplified in all the three stages of calli showing its importance in SE as it is required for the formation and maturation of embryos (Figure 4a, b). If the Phylogenetic analyses of the predicted sequences is performed, then that may be helpful to analyze or characterize the genetic role for accurate functioning of the gene for regeneration potential in the local varieties as has been observed by Dev et al (2018) in Eggplant.…”