Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia

Ichikawa‐Seki, Madoka; Allamanda, Puttik; Wibowo, Putut Eko; Mohanta, Uday Kumar; Sodirun,; Guswanto, Azirwan; Nishikawa, Yoshifumi

doi:10.1016/j.parint.2016.06.004

Cited by 22 publications

(18 citation statements)

References 17 publications

(46 reference statements)

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“…Phylogenetic analysis of nad 1 sequences suggests that haplotypes of F. gigantica found in Asian countries can be categorized into three haplogroups: A, B and C [20, 44, 51, 52]. Haplogroup A is found in Indian subcontinent, including Nepal and Bangladesh [20, 52], while all three haplotypes are found in Southeast Asia, including Thailand and Myanmar [44, 51, 53, 54]. Our analysis suggested that Egyptian haplotypes of F. gigantica formed a monophyletic clade sister to those from Asia to the exclusion of Zambian samples (Fig.…”

Section: Discussionmentioning

confidence: 99%

Identity of Fasciola spp. in sheep in Egypt

et al. 2016

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BackgroundIn Egypt, liver flukes, Fasciola spp. (Digenea: Fasciolidae), have a serious impact on the farming industry and public health. Both Fasciola hepatica and Fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. Little is known on the identity and genetic variability of Fasciola populations in sheep.MethodsThis study was performed to determine the prevalence of liver flukes in sheep in Menofia Province as a representative area of the delta region in Egypt, as measured by postmortem examination of slaughtered animals at three abattoirs. The identity and genetic variability of Fasciola spp. in slaughtered animals were determined by PCR-sequence analysis of the nuclear ribosomal internal transcribed spacer 1 (ITS1) and the mitochondrial NADH dehydrogenase subunit 1 (nad1) genes.ResultsPhysical inspection of the liver indicated that 302 of 2058 (14.7%) slaughtered sheep were infected with Fasciola spp. Sequence analysis of the ITS1 and nad1 genes of liver flukes from 17 animals revealed that 11 animals were infected with F. hepatica, four with F. gigantica, and two with both species. Seventy eight of 103 flukes genetically characterized from these animals were F. hepatica, 23 were F. gigantica, and two had ITS1 sequences identical to F. hepatica but nad1 sequences identical to F. gigantica. nad1 sequences of Egyptian isolates of F. gigantica showed pronounced differences from those in the GenBank database. Egyptian F. gigantica haplotypes formed haplogroup D, which clustered in a sister clade with haplogroups A, B and C circulating in Asia, indicating the existence of geographic isolation in the species.ConclusionsBoth F. hepatica and F. gigantica are prevalent in sheep in Egypt and an introgressed form of the two occurs as the result of genetic recombination. In addition, a geographically isolated F. gigantica population is present in the country. The importance of these observations in epidemiology of fascioliasis needs to be examined in future studies.

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Section: Discussionmentioning

confidence: 99%

Identity of Fasciola spp. in sheep in Egypt

et al. 2016

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“…taxa in South West of Asia, in India and Bangladesh, near the studied region of Iran, but The Fg type of these flukes was detected in aspermic Fasciola sp. using ITS-RFLP, and also the phylogenetic study with ND1 gene showed that they were placed in F. gigantica complex (8,9). These flukes were probably considered as abnormal F. gigantica with oligozoospermia, which might have occurred because of the aging of flukes.…”

Section: Discussionmentioning

confidence: 93%

“…Morphological criteria such as body size and shape are among the traditional and important methods to distinguish between the 2 species, but these methods are not commonly trusted because of the variable range in different species (6 (8,9).…”

Section: Introductionmentioning

confidence: 99%

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Rouhani

Raeghi

Harandi

et al. 2017

Arch Clin Infect Dis

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Background: Fascioliasis is a neglected zoonotic disease with worldwide distribution. Phylogenetic analysis of Fasciola species is critical because of their different epidemiology. Objectives: The current study aimed at identifying Fasciola spp. based on spermatogenesis and phylogenetic analysis of mitochondrial (ND1) gene. Methods: One hundred and fifty adults with fascioliasis were selected from the Eastern provinces of Iran, based on spermatogenetic ability, measurement criteria, and ITS (nuclear ribosomal DNA internal transcribed spacers) 1 gene restriction fragment length polymorphism (RFLP) pattern. Then, their genetic diversity indices and phylogenetic relationships were analyzed using mitochondrial DNA marker of Fasciola population spread in the East of Iran. Results: Aspermic F. gigantica was observed in the eastern part of Iran. Partial sequences of mtDNA showed new haplotypes in both species. Pairwise fixation index between different F. gigantica populations calculated from the nucleotide data set of ND1 gene were statistically significant and showed the genetic differences in pairwise population. Conclusions:The results of the study showed that F. hepatica, lives in this region of Iran, had different genetic structures compared with the other Fasciola populations in the world. Fasciola gigantica present in the East of Iran had different genetic structures, compared with the other Fasciola population, based on genetic index.

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“…Over past few decades, high levels of animal movement have been reported in domestic ruminants in several European and Asian countries (Kelley et al, 2016;Vilas et al, 2012); hence genetic analyses are needed to understand the corresponding spread of F. gigantica infections and aid in the development of parasite control strategies (Hayashi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and multiplicity of infection in Fasciola gigantica isolates of Pakistani livestock

Rehman

Zahid

Rashid

et al. 2020

Parasitology International

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Fasciola gigantica liver flukes are responsible for over 3 billion US dollars of production loss annually in farmed livestock and cause widespread zoonotic disease. Nevertheless, the understating of the emergence and spread of the trematode species is poor. The multiplicity of F. gigantica infection and its spread is potentially influenced by multiple factors, including the abundance of suitable intermediate hosts, climatic conditions favoring the completion of the parasite's lifecycle, and translocation of infected animals or free-living parasite stages between regions. Here we describe the development of a 'tremabiome' metabarcoding sequencing method to explore the numbers of F. gigantica genotypes per infection and patterns of parasite spread, based on genetic characteristics of the mitochondrial NADH dehydrogenase 1 (mt-ND-1) locus. We collected F. gigantica from three abattoirs in the Punjab and Balochistan provinces of Pakistan, and our results show a high level of genetic diversity in 20 F. gigantica populations derived from small and large ruminants consigned to slaughter in both provinces. This implies that F. gigantica can reproduce in its definitive hosts through meiosis involving cross-and self-breeding, as described in the closely related species, Fasciola hepatica. The genetic diversity between the 20 populations derived from different locations also illustrates the impact of animal movements on gene flow. Our results demonstrate the predominance of single haplotypes, consistent with a single introduction of F. gigantica infection in 85% of the hosts from which the parasite populations were derived. This is consistent with clonal reproduction in the intermediate snail hosts.

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Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia

Cited by 22 publications

References 17 publications

Identity of Fasciola spp. in sheep in Egypt

Identity of Fasciola spp. in sheep in Egypt

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Genetic diversity and multiplicity of infection in Fasciola gigantica isolates of Pakistani livestock

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