Molecular Characterization and Toxicity Confirmation of LukM/F′-PV Producing Staphylococcus aureus Isolated from Bovine Mastitis Samples in Mysore, India

Halami, Prakash M.

doi:10.1007/s12088-013-0359-2

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…The presence and production of the S. aureus leukocidin LukMF′ is associated with bovine mastitis in several epidemiological studies ( 18 , 20 – 23 ). However, the mechanism of LukMF′ activity and its role in the pathophysiology of mastitis are not well understood.…”

Section: Discussionmentioning

confidence: 99%

“…LukMF′ is located within the genome of temperate phage φSa1 and can be horizontally transmitted between strains ( 18 , 19 ). The frequency of distribution of LukMF′ among bovine isolates ranges between 10 and 86% and differs according to geographic region and site of isolation ( 18 , 20 – 23 ). In cattle, the presence of LukMF′ genes is associated with mastitis-causing strains ( 18 , 20 – 23 ).…”

Section: Introductionmentioning

confidence: 99%

“…The frequency of distribution of LukMF′ among bovine isolates ranges between 10 and 86% and differs according to geographic region and site of isolation ( 18 , 20 – 23 ). In cattle, the presence of LukMF′ genes is associated with mastitis-causing strains ( 18 , 20 – 23 ). LukMF′ is thought to be a virulence factor involved in the pathogenesis of mastitis because of its lytic effect on neutrophils, the most important effector cells in preventing intramammary infections ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

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Bovine Staphylococcus aureus Secretes the Leukocidin LukMF′ To Kill Migrating Neutrophils through CCR1

Vrieling

Koymans

Heesterbeek

et al. 2015

mBio

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Although Staphylococcus aureus is best known for infecting humans, bovine-specific strains are a major cause of mastitis in dairy cattle. The bicomponent leukocidin LukMF′, exclusively harbored by S. aureus of ruminant origin, is a virulence factor associated with bovine infections. In this study, the molecular basis of the host specificity of LukMF′ is elucidated by identification of chemokine receptor CCR1 as its target. Bovine neutrophils, the major effector cells in the defense against staphylococci, express significant cell surface levels of CCR1, whereas human neutrophils do not. This causes the particular susceptibility of bovine neutrophils to pore formation induced by LukMF′. Bovine S. aureus strains produce high levels of LukMF′ in vitro. In culture supernatant of the mastitis field isolate S1444, LukMF′ was the most important cytotoxic agent for bovine neutrophils. In a fibrin gel matrix, the effects of the in situ secreted toxins on neutrophils migrating toward S. aureus were visualized. Under these physiological ex vivo conditions, bovine S. aureus S1444 efficiently killed approaching neutrophils at a distance through secretion of LukMF′. Altogether, our findings illustrate the coevolution of pathogen and host, provide new targets for therapeutic and vaccine approaches to treat staphylococcal diseases in the cow, and emphasize the importance of staphylococcal toxins in general.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine Staphylococcus aureus Secretes the Leukocidin LukMF′ To Kill Migrating Neutrophils through CCR1

Vrieling

Koymans

Heesterbeek

et al. 2015

mBio

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“…The carbohydrate fermentation pattern was assessed by using HiMedia sugar discs with 20 mg/ml concentration (cellobiose, lactose, dextrose, adonitol, inulin, trehalose, melibiose, galactose, inositol, fructose, salicin, sorbitol, sucrose, xylose, rhamnose, mannitol, arabinose, mannose, maltose, raffinose, dulcitol). The safety evaluation of LAB was performed by microbiological detection of haemolytic, gelatinase and lecithinase activity on nutrient agar plates supplemented with 7 % defibrinated sheep blood, Todd-Hewitt agar containing 3 % of gelatin and nutrient agar with 5 % egg yolk emulsion as well as with 1 % potassium tellurite, respectively, as described earlier [19][20][21]. Staphylococcus aureus 17A was used as a positive control for safety evaluation assays.…”

Section: Preliminary Characterization Of Probiotic Bacteriamentioning

confidence: 99%

Screening, Characterization and In Vitro Evaluation of Probiotic Properties Among Lactic Acid Bacteria Through Comparative Analysis

Devi

Archer

Halami

2015

Probiotics & Antimicro. Prot.

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The present work aimed to identify probiotic bacteria from healthy human infant faecal and dairy samples. Subsequently, an assay was developed to evaluate the probiotic properties using comparative genetic approach for marker genes involved in adhesion to the intestinal epithelial layer. Several in vitro properties including tolerance to biological barriers (such as acid and bile), antimicrobial spectrum, resistance to simulated digestive fluids and cellular hydrophobicity were assessed. The potential probiotic cultures were rapidly characterized by morphological, physiological and molecular-based methods [such as RFLP, ITS, RAPD and (GTG)5]. Further analysis by 16S rDNA sequencing revealed that the selected isolates belong to Lactobacillus, Pediococcus and Enterococcus species. Two cultures of non-lactic, non-pathogenic Staphylococcus spp. were also isolated. The native isolates were able to survive under acidic, bile and simulated intestinal conditions. In addition, these cultures inhibited the growth of tested bacterial pathogens. Further, no correlation was observed between hydrophobicity and adhesion ability. Sequencing of probiotic marker genes such as bile salt hydrolase (bsh), fibronectin-binding protein (fbp) and mucin-binding protein (mub) for selected isolates revealed nucleotide variation. The probiotic binding domains were detected by several bioinformatic tools. The approach used in the study enabled the identification of potential probiotic domains responsible for adhesion of bacteria to intestinal epithelial layer, which may further assist in screening of novel probiotic bacteria. The rapid detection of binding domains will help in revealing the beneficial properties of the probiotic cultures. Further, studies will be performed to develop a novel probiotic product which will contribute in food and feed industry.

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“…While the PVL genes are restricted to human strains, LukMF′ is associated with animal strains, especially with isolates from bovine mastitis1617. Reported prevalence of the lukMF ′ operon in bovine isolates ranges from 10–86% and differs per geographic region1617181920. LukMF′ has a very profound lytic effect on bovine neutrophils and monocytes21.…”

mentioning

confidence: 99%

LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

Vrieling

Boerhout

Wigcheren

et al. 2016

Sci Rep

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Staphylococcus aureus is a major human and animal pathogen and a common cause of mastitis in cattle. S. aureus secretes several leukocidins that target bovine neutrophils, crucial effector cells in the defence against bacterial pathogens. In this study, we investigated the role of staphylococcal leukocidins in the pathogenesis of bovine S. aureus disease. We show that LukAB, in contrast to the γ-hemolysins, LukED, and LukMF′, was unable to kill bovine neutrophils, and identified CXCR2 as a bovine receptor for HlgAB and LukED. Furthermore, we assessed functional leukocidin secretion by bovine mastitis isolates and observed that, although leukocidin production was strain dependent, LukMF′ was most abundantly secreted and the major toxin killing bovine neutrophils. To determine the role of LukMF′ in bovine mastitis, cattle were challenged with high (S1444) or intermediate (S1449, S1463) LukMF′-producing isolates. Only animals infected with S1444 developed severe clinical symptoms. Importantly, LukM was produced in vivo during the course of infection and levels in milk were associated with the severity of mastitis. Altogether, these findings underline the importance of LukMF′ as a virulence factor and support the development of therapeutic approaches targeting LukMF′ to control S. aureus mastitis in cattle.

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Molecular Characterization and Toxicity Confirmation of LukM/F′-PV Producing Staphylococcus aureus Isolated from Bovine Mastitis Samples in Mysore, India

Cited by 8 publications

References 13 publications

Bovine Staphylococcus aureus Secretes the Leukocidin LukMF′ To Kill Migrating Neutrophils through CCR1

Bovine Staphylococcus aureus Secretes the Leukocidin LukMF′ To Kill Migrating Neutrophils through CCR1

Screening, Characterization and In Vitro Evaluation of Probiotic Properties Among Lactic Acid Bacteria Through Comparative Analysis

LukMF′ is the major secreted leukocidin of bovine Staphylococcus aureus and is produced in vivo during bovine mastitis

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