Molecular characterization and volatile binding properties of pheromone binding proteins and general odorant binding proteins in Conogethes pinicolalis (Lepidoptera: Crambidae)

Jing, Dapeng; Zhang, Tiantao; Prabu, Sivaprasath; Bai, Shuxiong; He, Kanglai; Wang, Zhenying

doi:10.1016/j.ijbiomac.2019.12.248

Cited by 26 publications

(22 citation statements)

References 57 publications

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“…However, some studies have demonstrated that GOBPs can interact with sex pheromones and possibly responsible for conducting the function [38]. Our another study have showed that PBP2 and GOBP1 genes may play similar roles in detecting and transporting sex pheromones and host plant volatiles in C. pinicolalis [39]. There are also evolutionary evidence that GOBPs may evolved from PBP by gene duplication, PBP and GOBP2 in Manduca sexta show close relationship and play an important role in coordinated olfactory behaviors [40,41].…”

Section: Discussionmentioning

confidence: 99%

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

et al. 2020

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Background: Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results: Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions: Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.

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Section: Discussionmentioning

confidence: 99%

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

et al. 2020

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show abstract

“…However, some studies have demonstrated that GOBPs can interact with sex pheromones and possibly responsible for conducting the function [39]. Our another study have showed that PBP2 and GOBP1 genes may play similar roles in detecting and transporting sex pheromones and host plant volatiles in C. pinicolalis [40]. There are also evolutionary evidence that GOBPs may evolved from PBP by gene duplication, PBP and GOBP2 in Manduca sexta show close relationship and play an important role in coordinated olfactory behaviors [41][42].…”

Section: Discussionmentioning

confidence: 77%

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Jing

Zhang²,

Bai

et al. 2020

Preprint

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Background: Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms , especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results: Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis . The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis . Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions: Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis , which might be supportive for pest management studies in future.

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“…The insect olfaction system allows insects to recognize and track volatile cues from host plants and mates, avoid toxic compounds and evade their predators [19]. The OBPs play an important role in olfactory sensation and are essential for binding odorant molecules and facilitating their transport through the aqueous neuronal environment [29].…”

Section: Discussionmentioning

confidence: 99%

“…OBPs possess high-specificity binding affinity with different plant volatiles and sex pheromones. Based on fluorescence competitive binding assays and computational molecular docking methods, a wide range of volatile binding capabilities have been reported [19]. However, it is still of great significance to characterize both the GOBPs and PBPs from different target insects chosen for study because the insect olfactory system is complex.…”

Section: Sequence Analysis Of Trufobp4mentioning

confidence: 99%

Binding Properties of Odorant-Binding Protein 4 of Tirathaba rufivena to Areca catechu Volatiles

Zhou

Wang

Cui

et al. 2022

Plants

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Odorant-binding proteins (OBPs) play a key role in the olfactory system and are essential for mating and oviposition host selection. Tirathaba rufivena, a serious lepidopterous insect pest of the palm area in recent years, has threatened cultivations of Areca catechu in Hainan. Female-biased odorant-binding protein 4 of T. rufivena (TrufOBP4) expression was hypothesized to participate in the process of oviposition host recognition and localization. In this study, we cloned and analyzed the cDNA sequence of TrufOBP4. The predicted mature protein TrufOBP4 is a small, soluble, secretory protein and belongs to a classic OBP subfamily. Fluorescence binding assay results showed that TrufOBP4 had high binding abilities with the host plant volatiles, octyl methoxycinnamate, dibutyl phthalate, myristic acid and palmitic acid. These four components tend to dock in the same binding pocket based on the molecular docking result. The interactions and contributions of key amino acid residues were also characterized. This research provides evidence that TrufOBP4 might participate in the chemoreception of volatile compounds from inflorescences of A. catechu and can contribute to the integrated management of T. rufivena.

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Molecular characterization and volatile binding properties of pheromone binding proteins and general odorant binding proteins in Conogethes pinicolalis (Lepidoptera: Crambidae)

Cited by 26 publications

References 57 publications

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Binding Properties of Odorant-Binding Protein 4 of Tirathaba rufivena to Areca catechu Volatiles

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