Molecular characterization, antibiotic resistance pattern and biofilm formation of Vibrio parahaemolyticus and V. cholerae isolated from crustaceans and humans

Ahmed, Heba A.; Bayomi, Rasha M. El; Hussein, Mohamed A.; Khedr, Mariam H.E.; Remela, Etab M. Abo; El‐Ashram, Ahmed

doi:10.1016/j.ijfoodmicro.2018.03.013

Cited by 78 publications

(62 citation statements)

References 64 publications

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“…The recent emergence and spread of antibiotic resistance reported among V. parahaemolyticus (Li et al, 2014;Letchumanan et al, 2015;Ahmed et al, 2018) is certainly the most striking evolution that has arisen in this pathogenic bacteria. The emergence of multidrug-resistant phenotypes among V. parahaemolyticus in many countries is an increasing concern (Letchumanan et al, 2014;Elmahdi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

et al. 2020

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Narayanan et al. V. parahaemolyticus With Pathogenic Potential in Seafood The conventional methods used to identify the pathogenic V. parahaemolyticus would be good for clinical isolates, but a more elaborate method is recommended for the detection of tdh gene in environmental isolates. This is the first comprehensive study on pathogenic V. parahaemolyticus in Kerala, India and demonstrates for the first time, the isolation of potentially pathogenic V. parahaemolyticus, carrying tdh gene from seafood collected from retail markets in Kerala.

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Section: Introductionmentioning

confidence: 99%

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

et al. 2020

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“…Regarding virulence-associated factors, hemolytic activity has been reported [12]. Also vibrios are included in the marine microbial community and they are able to cause severe or mild gastroenteritis in humans [17,18]. Most of the species belonging to the genus Vibrio are harmless to humans except for the "big four" representing by V. cholerae, V. parahaemolyticus, V. vulnificus and V. alginolyticus [19].…”

Section: Introductionmentioning

confidence: 99%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

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Antimicrobial resistance is a major public health concern restricted not only to healthcare settings but also to veterinary and environmental ones. In this study, we analyzed, by whole genome sequencing (WGS) the resistome, mobilome and virulome of 12 multidrug-resistant (MDR) marine strains belonging to Shewanellaceae and Vibrionaceae families collected at aquaculture centers in Italy. The results evidenced the presence of several resistance mechanisms including enzyme and efflux pump systems conferring resistance to beta-lactams, quinolones, tetracyclines, macrolides, polymyxins, chloramphenicol, fosfomycin, erythromycin, detergents and heavy metals. Mobilome analysis did not find circular elements but class I integrons, integrative and conjugative element (ICE) associated modules, prophages and different insertion sequence (IS) family transposases. These mobile genetic elements (MGEs) are usually present in other aquatic bacteria but also in Enterobacteriaceae suggesting their transferability among autochthonous and allochthonous bacteria of the resilient microbiota. Regarding the presence of virulence factors, hemolytic activity was detected both in the Shewanella algae and in Vibrio spp. strains. To conclude, these data indicate the role as a reservoir of resistance and virulence genes in the environment of the aquatic microbiota present in the examined Italian fish farms that potentially might be transferred to bacteria of medical interest.

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“…While oral rehydration therapy is critical for case management, complementing with antibiotics to eradicate vibrios is important in the management, prevention of spread and control of the disease [6]. Recent reports indicate the emergence of antibiotic resistant V. cholerae strains from cholera-endemic African countries [7,8].…”

Section: Introductionmentioning

confidence: 99%

A molecular and epidemiological study of Vibrio cholerae isolates from cholera outbreaks in southern Ghana

Asare¹,

Otchere²,

Akyeh³

et al. 2020

PLoS ONE

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Cholera remains a major global public health threat and continuous emergence of new Vibrio cholerae strains is of major concern. We conducted a molecular epidemiological study to detect virulence markers and antimicrobial resistance patterns of V. cholerae isolates obtained from the 2012-2015 cholera outbreaks in Ghana. Archived clinical isolates obtained from the 2012, 2014 and 2015 cholera outbreaks in Ghana were revived by culture and subjected to microscopy, biochemical identification, serotyping, antibiotic susceptibility testing, molecular detection of distinct virulence factors and Multi-Locus Variable-Number of Tandem-Repeat Analysis (MLVA). Of 277 isolates analysed, 168 (60.6%) were confirmed to be V. cholerae and 109 (39.4%) isolates constituted other bacteria (Escherichia coli, Aeromonas sobria, Pseudomonas aeruginosa, Enterobacter cloacae and Enterococci faecalis). Serotyping the V. cholerae isolates identified 151 (89.9%) as Ogawa, 3 (1.8%) as Inaba and 14 (8.3%) as non-O1/O139 serogroup. The O1 serogroup isolates (154/168, 91.7%) carried the cholera toxin ctxB gene as detected by PCR. Additional virulence genes detected include zot, tcpA, ace, rtxC, toxR, rtxA, tcpP, hlyA and tagA. The most common and rare virulence factors detected among the isolates were rtxC (165 isolates) and tcpP (50 isolates) respectively. All isolates from 2014 and 2015 were multidrug resistant against the selected antibiotics. MLVA differentiated the isolates into 2 large unique clones A and B, with each predominating in a particular year. Spatial analysis showed clustering of most isolates at Ablekuma sub-district. Identification of several virulence genes among the two different genotypes of V. cholerae isolates and resistance to first-and second-line antibiotics, calls for scaleup of preventive strategies to reduce transmission, and strengthening of public health laboratories for rapid antimicrobial susceptibility testing to guide accurate treatment.

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Molecular characterization, antibiotic resistance pattern and biofilm formation of Vibrio parahaemolyticus and V. cholerae isolated from crustaceans and humans

Cited by 78 publications

References 64 publications

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

A molecular and epidemiological study of Vibrio cholerae isolates from cholera outbreaks in southern Ghana

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