Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum

Wei, Qianyu; Mu, Changkao; Zhao, Jianmin

doi:10.1016/j.fsi.2018.07.036

Cited by 9 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…Lysozymes are found ubiquitously across the animal kingdom and have important roles in host immune responses against bacterial infection ( 45 – 48 ). Expression of the lysozyme gene in R. philippinarum was significantly upregulated after V. anguillarum infection, peaking at 48 h after infection, and then decreasing by 72 h, although the overall expression remained higher than that in the control group ( 48 ). In the current study, expression of the lysozyme gene in the VaR group was significantly lower than that in the Con group; thus, we speculate that there are temporal patterns in its expression similar to those seen for GST gene expression.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

et al. 2022

View full text Add to dashboard Cite

The clam Ruditapes philippinarum is an important species in the marine aquaculture industry in China. However, in recent years, the aquaculture of R. philippinarum has been negatively impacted by various bacterial pathogens. In this study, the transcriptome libraries of R. philippinarum showing different levels of resistance to challenge with Vibrio anguillarum were constructed and RNA-seq was performed using the Illumina sequencing platform. Host immune factors were identified that responded to V. anguillarum infection, including C-type lectin domain, glutathione S-transferase 9, lysozyme, methyltransferase FkbM domain, heat shock 70 kDa protein, Ras-like GTP-binding protein RHO, C1q, F-box and BTB/POZ domain protein zf-C2H2. Ten genes were selected and verified by RT-qPCR, and nine of the gene expression results were consistent with those of RNA-seq. The lectin gene in the phagosome pathway was expressed at a significantly higher level after V. anguillarum infection, which might indicate the role of lectin in the immune response to V. anguillarum. Comparing the results from R. philippinarum resistant and nonresistant to V. anguillarum increases our understanding of the resistant genes and key pathways related to Vibrio challenge in this species. The results obtained here provide a reference for future immunological research focusing on the response of R. philippinarum to V. anguillarum infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In the present study, the A. jandaei infection could rapidly up‐regulate the expression of Ps lysC in liver and blood cells, indicating its involvement in the immune response against bacterial infections. Many previous studies have demonstrated that under the challenge of various bacteria, LPS and β‐glucan, the expression of C‐type lysozyme could be significantly enhanced (Paulsen, Engstad, & Robertsen, ; Paulsen, Lunde, Engstad, & Robertsen, ; Wei, Mu, Wang, & Zhao, ). So it is an indisputable fact that c‐lysozyme can participate in the body's immune response against bacterial infections.…”

Section: Discussionmentioning

confidence: 99%

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Yuan

et al. 2019

Aquac Res

View full text Add to dashboard Cite

Lysozymes play vital roles in humoural immune response against bacterial invasion by its lytic activity. In the present study, a new C‐type lysozyme was identified and characterized from Chinese soft‐shelled turtle Pelodiscus sinensis. The full‐length cDNA of PslysC was of 923 bp, encoding a polypeptide of 148 amino acid residues. The multiple alignments and phylogenetic relationship analysis revealed the highly enzyme‐related conserved residues. The real‐time PCR analysis suggested that PslysC was constitutively expressed in a wide range of tissues with highest level in blood cells and liver. The expression of PslysC could be significantly up‐regulated under Aeromonas jandaei infection and ammonia exposure, while no significant changes were found under Poly I:C infection. The rPslysC protein was expressed in E. coli and purified by Ni‐NTA. The optimal pH and temperature for rPslysC protein lytic activities were determined at pH 7 and 30℃. rPslysC can inhibit the growth of eight kinds of Gram‐negative bacteria, and three kinds of Gram‐positive bacteria. The binding activity of rPslysC to different microbial polysaccharides and microorganism was analysed. The results showed that rPslysC could bind to selected bacteria, and exhibit a strong binding activity to lipopolysaccharide and peptidoglycan, but a weak binding activity to β‐glucan. This suggests that the binding activity might be the major mechanism of action to realize the antibacterial activity. The present study will provide helpful evidence to further understand the innate immunity of P. sinensis, and the interaction mechanisms of C‐type lysozymes with bacterial membranes.

show abstract

“…The c-type lysozyme was first extracted from chicken egg white, and the g-type lysozyme was identified in egg white from Embden goose (Xie et al, 2019). Furthermore, c-and g-type lysozymes have been previously reported in various marine animals, such as Yangtze sturgeon (Acipenser dabryanus) (Zhang et al, 2018), longtooth grouper (Epinephelus bruneus) (Harikrishnan et al, 2011), orange-spotted grouper (Epinephelus coioides) (Wei et al, 2012), Manila clam (Ruditapes philippinarum) (Wei et al, 2018), Japanese flounder (Paralichthys olivaceus) (Hikima et al, 2001), seahorse (Hippocampus abdominalis) (Ko et al, 2016), and turbot (Scophthalmus maximus) (Zhao et al, 2011). However, very few reports reveal the in vitro antibacterial activity of c-type lysozyme and g-type-like lysozyme in ornamental fish (Smith et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Functional characterization and expression analysis of c-type and g-like-type lysozymes in yellowtail clownfish (Amphiprion clarkii)

Kim¹,

Sohn²,

Omeka³

et al. 2023

Fish Aquat Sci

View full text Add to dashboard Cite

Lysozymes are well-known antibacterial enzymes that mainly target the peptidoglycan layer of the bacterial cell wall. Animal lysozymes are mainly categorized as g-type, c-type, and i-type based on protein sequence and structural differences. In this study, c-type (AcLysC) and g-like-type (AcLysG-like) lysozymes from Amphiprion clarkii were characterized in silico via expressional and functional approaches. According to in silico analysis, open reading frames of AcLysC and AcLysG-like were 429 bp and 570 bp, respectively, encoding the corresponding polypeptide chains with 142 and 189 amino acids. Elevated expression levels of AcLysC and AcLysG-like were observed in the liver and the heart tissues, respectively, as evidenced by quantitative real-time polymerase chain reaction assays. AcLysC and AcLysG-like transcript levels were upregulated in gills, head kidney, and blood cells following experimental immune stimulation. Recombinant AcLysC exhibited potent lytic activity against Vibrio anguillarum, whereas recombinant AcLysG-like showed remarkable antibacterial activity against Vibrio harveyi and Streptococcus parauberis, which was further evidenced by scanning electron microscopic imaging of destructed bacterial cell walls. The findings of this study collectively suggest the potential roles of AcLysC and AcLysG-like in host immune defense.

show abstract

Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum

Cited by 9 publications

References 19 publications

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Functional characterization and expression analysis of c-type and g-like-type lysozymes in yellowtail clownfish (Amphiprion clarkii)

Contact Info

Product

Resources

About