Molecular characterization, expression, and functional analysis of chicken TRAF6

Jin, Jie; Ran, Jin-Shan; Yang, Chaowu; Jiang, Xiaosong; Zhou, Yi; Feng, Zeqing; Wang, Y.; Lan, Dan; Ren, Peng; Liu, Y.P.

doi:10.4238/gmr16019138

Cited by 3 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In humans, the family has evolved into seven molecules with various functions. Moreover, there are seven TRAFs in sea urchins, eight in zebrafish, and 24 members in amphioxus (15)(16)(17)(18). By conducting similarity searches and homology inferences from the currently available genome and transcriptome data, we identified twelve traf genes in the genome of sea lampreys (P. marinus), five traf genes in the Japanese lampreys (Lampetra japonicum) transcriptome, and ten different traf sequences in the transcriptomes of reissner lamprey (Lethenteron reissneri).…”

Section: Results Traf Family In Lampreysmentioning

confidence: 99%

“…Motif 10, 13, and 14 are involved in the formation of the Zn-Finger domain. Motif 12,15,18,or 19 are related to the coiled-coil domain. Motif1, 2, 3, 5, and 8 are involved in the formation of the MATH-TRAF domain.…”

Section: Results Traf Family In Lampreysmentioning

confidence: 99%

“…In addition, the mRNA expression of TRAF3 is first increased and then reduced in sea cucumbers after receiving Gram-negative bacteria or LPS stimulation (10). TRAF3 was significantly elevated in chickens after polyI:C stimulation (15). Different tissue distributions and stimuli responses of lamprey TRAFs and RIPKs indicate that they participate in different immune responses in the species.…”

Section: Discussionmentioning

confidence: 96%

“…As TRAF molecules are important immunological molecules, they have been identified in both invertebrates and vertebrates, such as mussels (5), pearl oysters (6), fish (8)(9)(10)(11)(12)(13), chickens (15), and pigeons (14,16). All of the TRAF protein family members TRAF1 to TRAF7 are presented in zebrafish (36).…”

Section: Discussionmentioning

confidence: 99%

“…The present research has focused on the function of TRAF2, TRAF3, and TRAF6 in inflammation, immunity, and tumors in humans. The TRAF gene family has been reported from invertebrates to vertebrates, such as bivalvia (5,6), cephalochorda (7), pisces (8)(9)(10)(11)(12)(13), aves (14)(15)(16), and mammalia (7).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Comprehensive Evolutionary Analysis of Lamprey TNFR-Associated Factors (TRAFs) and Receptor-Interacting Protein Kinase (RIPKs) and Insights Into the Functional Characterization of TRAF3/6 and RIPK1

Hou

Pang

2020

Front. Immunol.

View full text Add to dashboard Cite

TNFR-associated factors (TRAFs) and receptor-interacting protein kinases (RIPKs) are important immunological linker molecules in mammals and play important roles in the TNFα, TLR and IFN signaling pathways. However, the evolutionary origins of these genes in vertebrates have not previously been described in lampreys. In this study, we searched the genomes of Lampetra japonicum, Lethenteron reissneri, and Petromyzon marinus for genes encoding trafs and ripks and performed homologous sequence alignment, phylogenetic tree, functional domain, conserved motif, gene structure, and synteny analyses to determine their evolutionary relationships. The distribution of the lamprey traf and ripk families and the immune response of the gene families in lampreys stimulated by different pathogens were also demonstrated, suggesting a role of structural changes in expression and functional diversification. Additionally, the dual luciferase reporter gene assay showed that the addition of exogenous immunomodulator (TNFα or IFN) to the overexpression of LjLRIPK1a or LjTRAF3/6 significantly downregulated NF-κB or ISRE activation. LjRIPK1a can significantly enhance caspase-8 activity, and overexpression of LjRIPK1a or LjTRAF3a/6 in HEK293T cells results in cell apoptosis. In summary, this study makes an important contribution to the understanding of the traf and ripk gene families in different vertebrates. Our results also provide new evidence for the evolution of vertebrate TRAFs and RIPKs and their impacts on immune regulation.

show abstract

Section: Results Traf Family In Lampreysmentioning

confidence: 99%

Section: Results Traf Family In Lampreysmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comprehensive Evolutionary Analysis of Lamprey TNFR-Associated Factors (TRAFs) and Receptor-Interacting Protein Kinase (RIPKs) and Insights Into the Functional Characterization of TRAF3/6 and RIPK1

Hou

Pang

2020

Front. Immunol.

View full text Add to dashboard Cite

show abstract

Evolution of RNA sensing receptors in birds

Magor

2022

Immunogenetics

View full text Add to dashboard Cite

Birds are important hosts for many RNA viruses, including influenza A virus, Newcastle disease virus, West Nile virus and coronaviruses. Innate defense against RNA viruses in birds involves detection of viral RNA by pattern recognition receptors. Several receptors of different classes are involved, such as endosomal toll-like receptors and cytoplasmic retinoic acid-inducible gene I-like receptors, and their downstream adaptor proteins. The function of these receptors and their antagonism by viruses is well established in mammals; however, this has received less attention in birds. These receptors have been characterized in a few bird species, and the completion of avian genomes will permit study of their evolution. For each receptor, functional work has established ligand specificity and activation by viral infection. Engagement of adaptors, regulation by modulators and the supramolecular organization of proteins required for activation are incompletely understood in both mammals and birds. These receptors bind conserved nucleic acid agonists such as single-or double-stranded RNA and generally show purifying selection, particularly the ligand binding regions. However, in birds, these receptors and adaptors differ between species, and between individuals, suggesting that they are under selection for diversification over time. Avian receptors and signalling pathways, like their mammalian counterparts, are targets for antagonism by a variety of viruses, intent on escape from innate immune responses.

show abstract

Biological characterization, expression, and functional analysis of tumor necrosis factor receptor-associated factor 6 in Nile tilapia ( Oreochromis niloticus )

Wang

Huang

et al. 2018

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Molecular characterization, expression, and functional analysis of chicken TRAF6

Cited by 3 publications

References 37 publications

Comprehensive Evolutionary Analysis of Lamprey TNFR-Associated Factors (TRAFs) and Receptor-Interacting Protein Kinase (RIPKs) and Insights Into the Functional Characterization of TRAF3/6 and RIPK1

Comprehensive Evolutionary Analysis of Lamprey TNFR-Associated Factors (TRAFs) and Receptor-Interacting Protein Kinase (RIPKs) and Insights Into the Functional Characterization of TRAF3/6 and RIPK1

Evolution of RNA sensing receptors in birds

Biological characterization, expression, and functional analysis of tumor necrosis factor receptor-associated factor 6 in Nile tilapia ( Oreochromis niloticus )

Contact Info

Product

Resources

About