Molecular Characterization of a dsRNA Mycovirus, Fusarium graminearum Virus-DK21, which Is Phylogenetically Related to Hypoviruses but Has a Genome Organization and Gene Expression Strategy Resembling Those of Plant Potex-like Viruses

Kwon, Sun‐Jung; Lim, Won-Seok; Park, Sang-Ho; Park, Mi-Ri; Kim, Kook‐Hyung

doi:10.1007/s10059-009-0112-1

Cited by 54 publications

(66 citation statements)

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“…Size and number of bands are consistent with the predicted genome structure for each virus. As common for mycoviruses -with the exception of Fusarium graminearum virus 1 (Kwon et al, 2007) -no subgenomic RNA could be detected in our total RNA preparations. Northern blot analysis of total RNA extracted from a number of fungal isolates (labels at the bottom of each panel).…”

Section: Rt-pcr and Northern Blot Analysismentioning

confidence: 70%

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Nerva¹,

et al. 2016

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Highlights• First report of mycoviruses isolated from fungi from marine environment.• Survey of mycoviruses using multiple approaches for both DNA and RNA genomes.• RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses.• Twelve new virus species were characterized molecularly.• Expression of a viral RNA from an endogenized cDNA segment. AbstractThe number of reported mycoviruses is increasing exponentially due to the current ability to detect mycoviruses using next-generation sequencing (NGS) approaches, with a large number of viral genomes built in-silico using data from fungal transcriptome projects. We decided to screen a collection of fungi originating from a specific marine environment (associated with the seagrass Posidonia oceanica) for the presence of mycoviruses: our findings reveal a wealth of diversity among these symbionts and this complexity will require further studies to address their specific role in this ecological niche. In specific, we identified twelve new virus species belonging to nine distinct lineages: they are members of megabirnavirus, totivirus, chrysovirus, partitivirus and five still undefined clades. We showed evidence of an endogenized virus ORF, and evidence of accumulation of dsRNA from metaviridae retroviral elements. We applied different techniques for detecting the presence of mycoviruses including (i) dsRNA extraction and cDNA cloning, (ii) small and total RNA sequencing through NGS techniques, (iii) rolling circle amplification (RCA) and total DNA extraction analyses, (iv) virus purifications and electron microscopy. We tried also to critically evaluate the intrinsic value and limitations of each of these techniques. Based on the samples we could compare directly, RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses. To our knowledge this is the first report on the virome of fungi isolated from marine environment.

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Section: Rt-pcr and Northern Blot Analysismentioning

confidence: 70%

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Nerva¹,

et al. 2016

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“…Particularmente, el uso de hongos endófitos, (Martínez-Álvarez, et al, 2016), tiene un futuro prometedor tanto por los resultados que proporciona (Bailey and Gilligan, 1997) como por la inocuidad en el ecosistema en que se emplee. Hasta el momento el control biológico ha sido empleado con éxito en el control de varias patologías vegetales (Trestić et al, 2001;Kwon et al, 2009;Romeralo, et al, 2013;Martínez-Álvarez, 2015).…”

Section: Introductionunclassified

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

Flores-Pacheco

2017

Rev. Científica FAREM-Estelí

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RESUMENLos hongos endófitos, son aquellos que colonizan los tejidos vivos internos de las plantas sin causar efecto significativo en el hospedante. Estos hongos, al ejercer en ocasiones, antagonismo frente a patógenos fúngicos a la planta hospedante, pueden ser una solución para el control de muchas de las enfermedades forestales causadas por otros hongos. Este campo ha sido poco evaluado en pruebas in vitro y en pruebas en vivo. Se utilizaron cinco hongos endófitos y seis hongos patógenos que se enfrentaron en medio de cultivo generalista Papa + Dextrosa +Agar (PDA) en condiciones de oscuridad a 25º C por siete días. Durante este periodo se midieron los ejes centrales y laterales de la colonia. Con estos datos se obtuvo el coeficiente de forma del hongo y se calculó el crecimiento directo, el coeficiente de forma del hongo patógeno frente al endófito respectivo, el porcentaje de inhibición del crecimiento radial y el porcentaje de inhibición de la zona de crecimiento. Se establecieron placas únicamente con el hongo patógeno a manera de grupo control. Los datos se analizaron con un Análisis de Varianza y para los casos estadísticamente significativos se aplicó la prueba de Diferencia Mínima Significativa. Los resultados muestran que los endófitos que tienen una mayor inhibición frente a los hongos patógenos. De esta manera se demuestra un efecto controlador de al menos un endófito sobre uno o más patógenos. Esto sugiere su posible utilidad como Agente de Control Biológico (ACB) ante enfermedades forestales. La reducción del crecimiento de los hongos patógenos ante la presencia de los endófitos, puede deberse a los mecanismos de acción de éstos (competencia y secreción de micotoxinas) frente al patógeno. Se deben realizar ensayos in vivo donde los endófitos se inoculen de acuerdo con su ecología y con un tiempo adecuado para la colonización de la planta.Palabras claves: control biológico, hongos patógenos, sanidad vegetal, antagonismo in vitro.

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“…Since the discovery of DRV1, several other positive-strand RNA mycoviruses such as Fusarium graminearum virus DK-21, Botrytis virus X, Oyster mushroom spherical virus, and two mycoviruses from Sclerotinia sclerotiorum have been discovered (Xie et al, 2006, Liu et al, 2009Howitt et al, 2006;Kwon et al, 2007;Yu et al, 2003). Although DRV1 has not been placed into a virus genus, its C-terminal half shows the highest similarity to Turnip crinkle virus (TCV) and Carnation mottle virus (CMV) in the genus Carmovirus, family Tombusviridae (Preisig et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

Moleleki

Wingfield

et al. 2011

Eur J Plant Pathol

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Molecular Characterization of a dsRNA Mycovirus, Fusarium graminearum Virus-DK21, which Is Phylogenetically Related to Hypoviruses but Has a Genome Organization and Gene Expression Strategy Resembling Those of Plant Potex-like Viruses

Cited by 54 publications

References 0 publications

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

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