Molecular characterization of a hepcidin homologue in starry flounder (Platichthys stellatus) and its synergistic interaction with antibiotics

Liu, Zi-Ming; Chen, Jie; Lv, Yao-Ping; Hu, Zehui; Dai, Qing-Min; Fan, Xiaoli

doi:10.1016/j.fsi.2018.09.019

Cited by 23 publications

(6 citation statements)

References 44 publications

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“…The mRNA expression levels of hepcidin in B. lenok [27] and S. trutta [28] significantly increased after they were infected with Aeromonas salmonicida and A. hydrophila . Platichthys stellatus hepcidin can inhibit the activity of V. parahaemolyticus and Edwardsella tarda [47].…”

Section: Discussionmentioning

confidence: 99%

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

Shen

Zhao

Zhao³

et al. 2019

IJMS

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Hepcidin is a liver-derived peptide hormone that is related to iron balance and immunity in humans. However, its function in Siniperca chuatsi has not been well elucidated. In this study, we analyzed the expression and function of the S. chuatsi hepcidin (Sc-hep) gene. Sc-hep was specifically expressed in the liver and appeared to be one of the most highly expressed genes in the liver. After spleen and kidney necrosis virus (ISKNV) infection and lipopolysaccharide (LPS) and polyinosinic—polycytidylic acid (Poly I:C) stimulation, the expression of Sc-hep in the liver increased by approximately 110, 6500, and 225 times, respectively. After ferrous sulfate (FS) injection, the expression of Sc-hep in the liver increased approximately 520-fold. We found that miR-19c-5p could inhibit Sc-hep expression. Five CpG dinucleotides distributed in the promoter region showed no differential methylation between the liver and the stomach, both presenting high methylation rates. After FS or LPS injection, the expression of three iron balance-related genes (FPN1, TFR1, and FTN) and five immune-related cytokine genes (IL-1β, IL8, TNF-α, TLR22, and SOCS3) significantly changed. These results indicate that Sc-hep participates in the regulation of iron balance and plays an important role in the immune system. Sc-hep increased approximately 52-fold when mandarin fish were domesticated with artificial diets. Sc-hep might be used as a real-time biomarker of mandarin fish liver because its expression markedly varies under different physiological conditions.

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Section: Discussionmentioning

confidence: 99%

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

Shen

Zhao

Zhao³

et al. 2019

IJMS

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“…Most notably, Ascl1 loss in maternal hepatocytes resulted in the following: (1) a complete depletion of Pseudobutyrivibrio –Roseburia intestinalis , which protects colonic mucosa against inflammation, 23 (2) the appearance of Desulfovibrio oxamicus–vulgaris , which metabolizes a variety of chemicals, 24 and (3) alterations in the relative abundance of 6 other bacteria species. Furthermore, RNA-seq analysis showed that Hamp2 , a gene encoding hepcidin antimicrobial peptide 2, which has a strong antimicrobial activity against certain bacteria, 25 , 26 depends on Ascl1 for expression. This finding was confirmed by qRT-PCR ( Figure 7 B ).…”

Section: Resultsmentioning

confidence: 99%

Activation of Proneuronal Transcription Factor Ascl1 in Maternal Liver Ensures a Healthy Pregnancy

Lee

García

Nambiar

et al. 2022

Cellular and Molecular Gastroenterology and Hepatology

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How the maternal liver adapts to pregnancy remains elusive. We found that maternal liver activates the expression of Ascl1, a gene encoding a proneuronal transcription factor, to coordinate the adaptations of maternal organs and the growth of the placenta, enabling a healthy pregnancy and normal postnatal growth of the offspring. BACKGROUND & AIMS:Maternal liver shows robust adaptations to pregnancy to accommodate the metabolic needs of the developing and growing placenta and fetus by largely unknown mechanisms. We found that Ascl1, a gene encoding a basic helix-loop-helix transcription factor essential for neuronal development, is highly activated in maternal hepatocytes during the second half of gestation in mice. METHODS: To investigate whether and how Ascl1Q7 plays a pregnancy-dependent role, we deleted the Ascl1 gene specifically in maternal hepatocytes from midgestation until term.RESULTS: As a result, we identified multiple Ascl1-dependent phenotypes. Maternal livers lacking Ascl1 showed aberrant hepatocyte structure, increased hepatocyte proliferation, enlarged hepatocyte size, reduced albumin production, and increased release of liver enzymes, indicating maternal liver dysfunction. Simultaneously, maternal pancreas and spleen and the placenta showed marked overgrowth; and the maternal ceca microbiome showed alterations in relative abundance of several bacterial subpopulations. Moreover, litters born from maternal hepatic Ascl1-deficient dams experienced abnormal postnatal growth after weaning, implying an adverse pregnancy outcome. Mechanistically, we found that maternal hepatocytes deficient for Ascl1 showed robust activation of insulin-like growth factor 2 expression, which may contribute to the Ascl1-dependent phenotypes widespread in maternal and uteroplacental compartments. CONCLUSIONS:In summary, we show that maternal liver, via activating Ascl1 expression, modulates the adaptations of maternal organs and the growth of the placenta to maintain a healthy pregnancy. Our studies show that Ascl1 is a novel and critical regulator of the physiology of pregnancy.

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“…Fish hepcidins are cysteine-rich peptides similar to human hepcidin with a hairpin structure linked via four disulfide bonds. They are iron-regulating antimicrobial hormones [ 104 , 105 ].…”

Section: Antimicrobial Peptides’ Natural Sourcementioning

confidence: 99%

An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

2022

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Antimicrobial peptides (AMPs) are constituents of the innate immune system in every kind of living organism. They can act by disrupting the microbial membrane or without affecting membrane stability. Interest in these small peptides stems from the fear of antibiotics and the emergence of microorganisms resistant to antibiotics. Through membrane or metabolic disruption, they defend an organism against invading bacteria, viruses, protozoa, and fungi. High efficacy and specificity, low drug interaction and toxicity, thermostability, solubility in water, and biological diversity suggest their applications in food, medicine, agriculture, animal husbandry, and aquaculture. Nanocarriers can be used to protect, deliver, and improve their bioavailability effectiveness. High cost of production could limit their use. This review summarizes the natural sources, structures, modes of action, and applications of microbial peptides in the food and pharmaceutical industries. Any restrictions on AMPs’ large-scale production are also taken into consideration.

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Molecular characterization of a hepcidin homologue in starry flounder (Platichthys stellatus) and its synergistic interaction with antibiotics

Cited by 23 publications

References 44 publications

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

Expression and Functional Analysis of Hepcidin from Mandarin Fish (Siniperca chuatsi)

Activation of Proneuronal Transcription Factor Ascl1 in Maternal Liver Ensures a Healthy Pregnancy

An Overview of the Potentialities of Antimicrobial Peptides Derived from Natural Sources

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