Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani

Chen, Xiangru; Yu, Zhaoyao; Sun, Yipeng; Yang, Meipeng; Jiang, Ning

doi:10.3389/fmicb.2022.978075

Cited by 4 publications

(4 citation statements)

References 62 publications

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“…A mass of partitiviruses have been found from a wide variety of phytopathogenic fungi, oomycetes, biological control fungi, and even human pathogenic fungi [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. Partitiviruses generally have a typical genome composed of two major dsRNA segments and each one contains a single ORF encoding RdRP or CP, respectively [ 18 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…A mass of partitiviruses have been found from a wide variety of phytopathogenic fungi, oomycetes, biological control fungi, and even human pathogenic fungi [8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Partitiviruses generally have a typical genome composed of two major dsRNA segments and…”

Section: Discussionmentioning

confidence: 99%

“…For the fungi host, the members of the family Partitiviridae have been reported from phytopathogenic fungi ( Aspergillus fumigatus , Botrytis cinerea , Cytospora sacchari , Rhizoctonia solani , Ustilaginoidea virens , etc. ), oomycete ( Pythium nunn ), biological control fungi ( Trichoderma harzianum ), and even human pathogenic fungi ( Talaromyces marneffei ) [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In general, the mycoviruses in this family contain two dsRNA segments which have a 1.4 to 2.4 kb genome.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Song,

Zhong,

Guan

et al. 2024

JoF

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Root rot as a result of Salvia miltiorrhiza is a common root disease caused by Fusarium spp., which has become one of the main diseases affecting the production of S. miltiorrhiza. Currently, several hypovirulence-related mycoviruses have been identified in many phytopathogenic fungi, including Fusarium spp., which show potential as biological controls. In this study, we report a new mycovirus, Fusarium oxysporum partitivirus 1 (FoPV1), isolated from F. oxysporum strain FCR51, which is a causal agent of S. miltiorrhiza dry rot. The FoPV1 genome contains two double-stranded RNA segments (dsRNA1 and dsRNA2). The size of dsRNA1 is 1773 bp, and it encodes a putative RNA-dependent RNA polymerase (RdRp). The dsRNA2 is 1570 bp in length, encoding a putative capsid protein (CP). Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of the RdRp and the CP proteins indicated that FoPV1 appears to be a new member of the family Partitiviridae that is related to members of the genus Gammapartitivirus. Pathogenicity assay showed that FoPV1 confers hypervirulence to its host, F. oxysporum. This is the first report of a partitivirus infecting F. oxysporum and the first hypovirulence-related mycovirus from the causal agent of S. miltiorrhiza dry rot.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Song,

Zhong,

Guan

et al. 2024

JoF

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“…Recently, tobacco target spot disease caused by R. solani was observed to proliferate in varying degrees in several regions of Guizhou province [4]. Research has revealed that R. solani has a broad host range and demonstrates strong saprophytic activity [5]. These findings underscore the urgency of addressing target spot disease in tobacco production.…”

Section: Introductionmentioning

confidence: 97%

Unveiling the Potential of Bacillus safensis Y246 for Enhanced Suppression of Rhizoctonia solani

Zhu,

Xu,

Wang

et al. 2023

JoF

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Rhizoctonia solani is a significant pathogen affecting various crops, including tobacco. In this study, a bacterial strain, namely Y246, was isolated from the soil of healthy plants and exhibited high antifungal activity. Based on morphological identification and DNA sequencing, this bacterial strain was identified as Bacillus safensis. The aim of this investigation was to explore the antifungal potential of strain Y246, to test the antifungal stability of Y246 by adjusting different cultivation conditions, and to utilize gas chromatography–mass spectrometry (GC-MS) to predict the volatile compounds related to antifungal activity in Y246. In vitro assays demonstrated that strain Y246 exhibited a high fungal inhibition rate of 76.3%. The fermentation broth and suspension of strain Y246 inhibited the mycelial growth of R. solani by 66.59% and 63.75%, respectively. Interestingly, treatment with volatile compounds derived from the fermentation broth of strain Y246 resulted in abnormal mycelial growth of R. solani. Scanning electron microscopy analysis revealed bent and deformed mycelium structures with a rough surface. Furthermore, the stability of antifungal activity of the fermentation broth of strain Y246 was assessed. Changes in temperature, pH value, and UV irradiation time had minimal impact on the antifungal activity, indicating the stability of the antifungal activity of strain Y246. A GC-MS analysis of the volatile organic compounds (VOCs) produced by strain Y246 identified a total of 34 compounds with inhibitory effects against different fungi. Notably, the strain demonstrated broad-spectrum activity, exhibiting varying degrees of inhibition against seven pathogens (Alternaria alternata, Phomopsis. sp., Gloeosporium musarum, Dwiroopa punicae, Colletotrichum karstii, Botryosphaeria auasmontanum, and Botrytis cinerea). In our extensive experiments, strain Y246 not only exhibited strong inhibition against R. solani but also demonstrated remarkable inhibitory effects on A. alternata-induced tobacco brown spot and kiwifruit black spot, with impressive inhibition rates of 62.96% and 46.23%, respectively. Overall, these findings highlight the significant antifungal activity of B. safensis Y246 against R. solani. In addition, Y246 has an excellent antifungal stability, with an inhibition rate > 30% under different treatments (temperature, pH, UV). The results showed that the VOCs of strain Y246 had a strong inhibitory effect on the colony growth of R. solani, and the volatile substances produced by strain Y246 had an inhibitory effect on R. solani at rate of 70.19%. Based on these results, we can conclude that Y246 inhibits the normal growth of R. solani. These findings can provide valuable insights for developing sustainable agricultural strategies.

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Identification and nucleotide sequencing of a novel partitivirus derived from Rhizoctonia solani AG-4 HG III isolate A14

Shi,

Dong,

Wang

et al. 2023

Arch Virol

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Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani

Cited by 4 publications

References 62 publications

Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Molecular Characterization of the First Partitivirus from a Causal Agent of Salvia miltiorrhiza Dry Rot

Unveiling the Potential of Bacillus safensis Y246 for Enhanced Suppression of Rhizoctonia solani

Identification and nucleotide sequencing of a novel partitivirus derived from Rhizoctonia solani AG-4 HG III isolate A14

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