Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

Abstract

The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method. The complete genome is 17 961 bp and is . Northern blot analysis and genomic sequence analysis indicated that segments 1-11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the J19 proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus strain with great significance to the evolution and origin of group B rotaviruses. INTRODUCTIONRotaviruses are important aetiological agents of disease in humans and animals. The viral genome is composed of 11 segments of double-stranded (ds) RNA that encode structural and non-structural proteins. The group A rotavirus strain SA11 genome, sequenced in 1990, is AUrich and has 18 555 bp (Both et al., 1984;Estes et al., 1984;Mitchell & Both, 1990). Its RNA segments encode six structural proteins (VP1, VP2, VP3, VP4, VP6 and VP7) and five non-structural proteins (NSP1, NSP2, NSP3, NSP4 and NSP5) (Estes, 2001). VP6 is the group antigen determinant, making up approximately 50 % of the viral protein (Estes et al., 1984;Estes & Cohen, 1989). The antigenic properties of VP6 are used to define seven antigenically different rotavirus groups, named groups A-G. Groups A, B and C rotaviruses infect humans, while other groups have been found only in animal species (Saif & Jiang, 1994). Group A rotaviruses cause severe diarrhoea in infants and young children, but they can also infect adults. Group C rotavirus infection occurs both in children and in adults, usually in sporadic cases or clustered outbreaks (Rodger et al., 1982;Caul et al., 1990;Jiang et al.,1995; Kuzuya et al., 1996; Nilsson et al., 2000;Adah et al., 2002;Chen et al., 2002;Ji et al., 2002;Schnagl et al., 2004).In China, large waterborne epidemics caused by the human group B rotavirus strain adult diarrhoea rotavirus (ADRV) infected thousands of people aged between 10 and 40 years of age in the 1980s (Hung et al., 1983(Hung et al., , 1984Chen et al., 1985Chen et al., , 1990 et al., 1987). The same rotavirus was implicated in gastroenteritis outbreaks in Beijing city in 1994 and in Shijiazhuang city in 1997. Partial genes of NADRV were clon...

The Genome Segments of a Group D Rotavirus Possess Group A-Like Conserved Termini but Encode Group-Specific Proteins

“…So far, nucleotide sequences are available only for group A, B, and C rotaviruses. Phylogenetic analysis of the sequences confirmed the suggested grouping of these viruses (30,31,69,74). In addition, group-specific conserved sequences are present at the termini of the genome segments.…”

“…In addition, several group A, B, and C rotaviruses have been characterized on the basis of their genome sequences, allowing a deeper insight into the evolutionary relationship of these viruses (1,31,42,43). The first genome sequence of the group D rotavirus presented here enables a more comprehensive classification of this virus compared to its former grouping based on serological criteria and limited terminal fingerprinting analysis (59).…”

“…Two additional tentative rotavirus groups (F and G) have been described but only scarcely characterized so far. Recently, novel rotavirus strains with limited sequence homologies to group B rotaviruses (strains J19 and B219) have been identified in adults with diarrhea but are without a final classification (31,51,77). Group A, B, and C rotaviruses are found in both humans and animals, whereas group E rotaviruses have been detected only in pigs and group D, F, and G rotaviruses have been detected only in birds (21,58,59,62,66).…”

Evidence for Occurrence of Human group B rotavirus in Central India Based on Characterization of NSP2 Gene

“…Analysis of the sequences of GBR strains showed that nucleotide sequence identity of MP-1 isolate of HuGBR was very high with Bang373 and CAL-1 (98.6 and 97.7%, respectively) and amino acid identity more than 93% with Bang373 and CAL-1 strains. The nucleotide and amino acid sequence identities with Chinese ADRV (WH-1) strain isolated in 2002 from Wuhan [24] was 92.8 and 92.5%, respectively, while identities with another Chinese ADRV strain (J19) [11] was considerably lower (45.6 and 48.3%, respectively). The sequences among Indian (MP-1 and CAL-1) and Bangladeshi (Bang 373) strains were more conserved, while point mutations were observed at several nucleotide positions (63, 66, 72, 76, 87, 144, 151 and 155) in ADRV (WH-1) strain, with change in amino acid at 26 (amino acid changed from V to I) and at 51 (amino acid changed from S to A) (Fig.…”

<b>Análise multigênica de rotavírus do grupo A em aves de criações comerciais brasileiras</b>

“…Dentro do grupo A, são observados elevados níveis de diversidade com alguns genes apresentando-se mais conservados que outros (JIANG et al, 2008).…”