Molecular characterization of a novel type of lipoxygenase (LOX) gene from common wheat (Triticum aestivum L.)

Feng, Bo; Dong, Zhenying; Xu, Zhibin; Wang, Daowen; Wang, Tao

doi:10.1007/s11032-011-9603-9

Cited by 17 publications

(24 citation statements)

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“…To identify the conserved regions of wheat LOX genes, three wheat LOX genes, TaLOX1, TaLOX2 and TaLOX3 (Feng et al 2010(Feng et al , 2012, were aligned with LOX sequences retrieved from three other three grass genomes, i.e., B. distachyon, rice and barley. Using the method described above, we identified 11 and 15 genes from B. distachyon and rice genome, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Hence, in the hexaploidy nature of common wheat (containing A, B and D subgenomes, which share *95 % sequence similarity), it is expected that probably more than 30 LOX copies could exist in common wheat genome. However, compared with such expectations, very few LOXs were mapped and isolated from common wheat so far (Feng et al 2010(Feng et al , 2012Garbus et al 2013). In addition, LOXs in subfamily II featured the presence of signal peptides, and their coding genes have only about 35 % overall sequence similarity with each other (Liavonchanka and Feussner 2006).…”

Section: Discussionmentioning

confidence: 98%

“…Those three known wheat LOX genes (TaLOX1, TaLOX2 and TaLOX3) and three barley LOX sequences were retrieved from Feng et al (2010Feng et al ( , 2012 and van Mechelen et al (1999), respectively. LOX genes from B. distachyon and rice genomes were determined by searches in database phytozome v9.1 (http://www.…”

Section: Methodsmentioning

confidence: 99%

“…LOX loci were initially identified and mapped to homeologous chromosome group 4 and 5 (designated as Lpx-1 and 2, respectively) (Hart and Langsten 1977;Li et al 1999). Recently, three LOX genes (TaLOX1, TaLOX2 and LOX3) from common wheat were isolated, and they were assigned to the short arm of chromosome 4D, the long arm of chromosome 5D, and chromosome 4A, respectively (Feng et al 2010(Feng et al , 2012. In durum wheat (Triticum turgidum, AABB), three copies of the LOX gene, Lpx-B1.1, Lpx-B1.2 and Lpx-B1.3, were identified in Lpx-B1 locus (Carrera et al 2007;Verlotta et al 2010), while only one LOX gene was found in Lpx-A1 locus of durum wheat (Garbus et al 2013).…”

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confidence: 99%

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Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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“…Given the rapid progress of modern molecular and biological techniques, many Lox genes have been mapped and cloned in cereal crops, including barley (Hordeum vulgare L.) (van Mechelen et al 1999), durum wheat (Triticum turgidum L.) (Carrera et al 2007;Garbus et al 2009;Verlotta et al 2010), and bread wheat (Triticum aestivum L.) (Feng et al 2010(Feng et al , 2012Geng et al 2012;Garbus et al 2013). To date, partial or complete sequences of eight Lox genes, namely, Lpx-A3 (DQ474242 and DQ474244),…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

Zhang¹,

Chen

Wu³

et al. 2015

Theor Appl Genet

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This study cloned two novel TaLox genes on chromosome of 4BS and developed a co-dominant marker, Lox-B23, in bread wheat that showed highly significant association with lipoxygenase activity. Lipoxygenase (Lox), a critical enzyme in the carotenoid biosynthetic pathway, significantly influences the color and processing quality of wheat-based products. Two novel Lox genes, designated TaLox-B2 and TaLox-B3, were cloned on chromosome 4BS of Chinese bread wheat. The deduced amino acid sequence showed that both TaLox-B2 and TaLox-B3 genes encoded an 861-aa protein and possessed a lipoxygenase superfamily domain at the 170-838 interval. Two different TaLox-B2 alleles, designated TaLox-B2a and TaLox-B2b, were subsequently discovered. A co-dominant marker, Lox-B23, was developed based on sequences of TaLox-B2a, TaLox-B2b, and TaLox-B3 genes to precisely distinguish these three alleles in Chinese bread cultivars. Among five allelic combinations of Lox genes at Lox-B1, Lox-B2, and Lox-B3 loci, wheat cultivars with TaLox-B1a/TaLox-B2a/TaLox-B3a combination exhibited the highest Lox activity, whereas those with TaLox-B1a/TaLox-B2b/TaLox-B3b combination significantly showed the lowest Lox activity. A RIL population was used to evaluate the influence of TaLox-B3a gene on Lox activity. Results showed that TaLox-B3a gene could significantly increase the Lox activity in bread wheat. Physical mapping indicated that both TaLox-B2 and TaLox-B3 genes were located on chromosome 4BS in bread wheat. This study provides useful information to further understand the molecular and genetic bases of Lox activity in bread wheat.

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Association analysis between Lipoxygenase activity and SSR markers in wheat grains

Zheng

Zhao

et al. 2021

CEREAL RESEARCH COMMUNICATIONS

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Lipoxygenase (LOX) activity is closely related to wheat processing and storage quality. In the present research, ten wheat cultivars were used to compare the effects of genotype, location, year, and their interactions on the LOX activity. Furthermore, 123 wheat cultivars were evaluated for LOX activity with 192 simple sequence repeat (SSR) markers and to identify elite alleles related to LOX activity. The results indicated that LOX activity was highly affected by genotype (variety) than that by the location. A total of 22 SSR molecular marker loci with a significant or very significant correlation with LOX activity were identified on performing association analysis. In 3 years, only one molecular marker locus associated with LOX activity was detected (WMC488); in 2 years, seven molecular marker loci were detected, while in only 1 year, the other 14 molecular marker loci were detected. A total of 7 and 6 marker loci significantly related to LOX activity accounting for 31.2% and 27.2%, respectively, were located in homologous groups 4 and 5, and group 7. This research provided the theoretical basis and the markers for molecular-assisted wheat breeding that facilitate the breeding process in the processing and storage quality of grains.

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Molecular characterization of a novel type of lipoxygenase (LOX) gene from common wheat (Triticum aestivum L.)

Cited by 17 publications

References 43 publications

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

Association analysis between Lipoxygenase activity and SSR markers in wheat grains

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