Molecular characterization of a novel fungal alphaflexivirus reveals potential inter-species horizontal gene transfer

Wu, Tun; Mao, Huiyan; Du, Hao; Chéng, Jiāsēn; Fù, Yànpíng; Liu, Yang; Xiè, Jiǎtāo

doi:10.1016/j.virusres.2023.199151

Cited by 1 publication

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“…For example, Sclerotinia sclerotiorum alphaflexivirus 2 was isolated from the hypovirulent strain S. sclerotiorum . The phylogenetic analysis results show that SsAFV2 clusters with Botrytis virus X (BVX) based on the multiple-sequence alignment of helicase, RdRp, and CP, but the methyltransferase of SsAFV2 was found to be most closely related to Sclerotinia sclerotiorum alphaflexivirus 1, suggesting that SsAFV2 is a new member of the Botrexvirus genus within the Alphaflexiviridae family [ 57 ]. However, more research is needed to determine why ambi-like viruses are so common among the macrofungi analyzed in further study.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Viromes Identified in Multiple Macrofungi

Zhou,

Zhang,

Deng

2024

Viruses

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Macrofungi play important roles in the soil elemental cycle of terrestrial ecosystems. Fungal viruses are common in filamentous fungi, and some of them can affect the growth and development of hosts. However, the composition and evolution of macrofungal viruses are understudied. In this study, ninety strains of Trametes versicolor, Coprinellus micaceus, Amanita strobiliformis, and Trametes hirsuta were collected in China. Four mixed pools were generated by combining equal quantities of total RNA from each strain, according to the fungal species, and then subjected to RNA sequencing. The sequences were assembled, annotated, and then used for phylogenetic analysis. Twenty novel viruses or viral fragments were characterized from the four species of macrofungi. Based on the phylogenetic analysis, most of the viral contigs were classified into ten viral families or orders: Barnaviridae, Benyviridae, Botourmiaviridae, Deltaflexiviridae, Fusariviridae, Hypoviridae, Totiviridae, Mitoviridae, Mymonaviridae, and Bunyavirales. Of these, ambi-like viruses with circular genomes were widely distributed among the studied species. Furthermore, the number and overall abundance of viruses in these four species of macrofungi (Basidiomycota) were found to be much lower than those in broad-host phytopathogenic fungi (Ascomycota: Sclerotinia sclerotiorum, and Botrytis cinerea). By employing metatranscriptomic analysis in this study, for the first time, we demonstrated the presence of multiple mycoviruses in Amanita strobiliformis, Coprinellus micaceus, Trametes hirsute, and Trametes versicolor, significantly contributing to research on mycoviruses in macrofungi.

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Section: Discussionmentioning

confidence: 99%