Molecular characterization of a pigeon paramyxovirus type 1 virus isolated from Eurasian collared doves in Iran, 2017

Dizaji, Reza Esmaeelzadeh; Molouki, Aidin; Hosseini, Hossein; Mehrabadi, Mohammad Hossein Fallah; Kafi, Zahra Ziafati; Takalou, Azin; Eram, Nava; Kumar, Niloufar; Ashuri, Alireza; Sadri, Naser

doi:10.4142/jvs.21254

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…The serotypes, classes, and genotypes as well as the year of isolation and the source of isolation are indicated: wild birds (W), pigeons (Pi), and poultry (Ch). If the pathogenicity of the virus was established in the cited work, then it is indicated by color: red indicates pathogenic isolates and green indicates non-pathogenic isolates [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , …”

Section: Introductionmentioning

confidence: 99%

An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022

Ртищев

Treshchalina

Shustova

et al. 2023

Veterinary Sciences

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In August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds of a particular farm died or were slaughtered after the onset of symptoms within a few days. Paramyxovirus was isolated from the diseased birds. Based on the nucleotide sequences of the F and NP gene fragments, it was determined that the virus belonged to subgenotype VII.1 AAvV-1 class II. The cleavage site of the F gene 109SGGRRQKRFIG119 and T in 546 and 555 position of the NP gene were typical for the velogenic type. The genetically closest NDV isolates were found in Iran. The mean time of death of 10-day-old chicken embryos upon infection with the minimal infectious dose was 52 h, which is typical for the velogenic pathotype. The virus caused 100% death of six-week-old chickens during oral infection as well as 100% mortality of all contact chickens, including those located in remote cages, which proves the ability of the virus to spread not only by the fecal–oral route but also by the aerosol route. That demonstrates a high level of pathogenicity and contagiousness of the isolated strain for chicken. However, mice intranasally infected with high doses of the virus did not die.

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Section: Introductionmentioning

confidence: 99%

An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022

Ртищев

Treshchalina

Shustova

et al. 2023

Veterinary Sciences

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RAA-CRISPR/Cas12a-Mediated Rapid, Sensitive, and Onsite Detection of Newcastle Disease in Pigeons

Liang,

Wang,

Gao

et al. 2024

Veterinary Sciences

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Pigeon Newcastle disease, caused by pigeon paramyxovirus type 1 (PPMV-1), is a significant infectious disease in pigeons that can result in substantial mortality and poses a severe threat to the pigeon industry. The rapid and accurate onsite diagnosis of pigeon disease is crucial for timely diagnosis and the implementation of effective prevention and control measures. In this study, we established a rapid detection method for PPMV-1 based on recombinase-aided amplification (RAA) and CRISPR/Cas12a. The RAA primers target the conserved regions of the L gene for preamplification in clinical nucleic acid samples, followed by CRISPR/Cas12a detection of the target gene. Visualization could be achieved by combination with a lateral flow dipstick (LFD). This method demonstrated high specificity, showing no cross-reactivity with non-PPMV-1 samples. The sensitivity of the method assessed by fluorescence analysis reached 100 copies/µL, and when it was combined with an LFD, the sensitivity was 103 copies/µL. The constructed RAA-CRISPR/Cas12a-LFD visual detection method was applied to clinical sample testing and was found to enable the rapid and accurate detection of swab samples and tissue specimens. Its sensitivity was consistent with the current gold standard, quantitative real-time PCR results. The RAA-CRISPR/Cas12a-LFD detection method we developed provides a novel approach for the rapid, simple, precise, and specific onsite diagnosis of pigeon Newcastle disease.

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Molecular characterization of a pigeon paramyxovirus type 1 virus isolated from Eurasian collared doves in Iran, 2017

Cited by 2 publications

References 21 publications

An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022

An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022

RAA-CRISPR/Cas12a-Mediated Rapid, Sensitive, and Onsite Detection of Newcastle Disease in Pigeons

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