Molecular Characterization of a Plasmid-Borne (pGT633) Erythromycin Resistance Determinant (ermGT) from Lactobacillus reuteri 100-63

Tannock, Gerald W.; Luchansky, John B.; Miller, Lynette; Connell, Hugh; Thode-Andersen, Soren; Mercer, Andrew A.; Klaenhammer, Todd R.

doi:10.1006/plas.1994.1007

Cited by 136 publications

(71 citation statements)

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“…The ⌬(umuDC)596::ermGT mutation (which confers resistance to erythromycin) was constructed in a K-12 uvrA6 strain, TK603 (22), with the same strategy used to generate the original ⌬(umuDC)595::cat mutation (43). Briefly, a 2.2-kb BamHI restriction fragment carrying the ermGT gene was obtained from plasmid pTRK95 (42) and was ligated into the unique BamHI site of plasmid pRW52 (43), generating plasmid pEC65. This plasmid carries the ermGT gene flanked by approximately 770 bp of the chromosomal E. coli sequences located immediately upstream of the umuDC operon and ϳ300 bp of downstream sequence.…”

Section: Methodsmentioning

confidence: 99%

In vivo stability of the Umu mutagenesis proteins: a major role for RecA

Frank¹,

Gonzalez²,

Ennis³

et al. 1996

J Bacteriol

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The Escherichia coli Umu proteins play critical roles in damage-inducible SOS mutagenesis. To avoid any gratuitous mutagenesis, the activity of the Umu proteins is normally kept to a minimum by tight transcriptional and posttranslational regulation. We have, however, previously observed that compared with an isogenic recA ؉ strain, the steady-state levels of the Umu proteins are elevated in a recA730 background (R. Woodgate and D. G. Ennis, Mol. Gen. Genet. 229:10-16, 1991). We have investigated this phenomenon further and find that another coprotease-constitutive (recA*) mutant, a recA432 strain, exhibits a similar phenotype. Analysis revealed that the increased steady-state levels of the Umu proteins in the recA* strains do indeed reflect an in vivo stabilization of the proteins. We have investigated the basis for the phenomenon and find that the mutant RecA* protein stabilizes the Umu proteins by not only converting the labile UmuD protein to the much more stable (and mutagenically active) UmuD protein but by directly stabilizing UmuD itself. In contrast, UmuC does not appear to be directly stabilized by RecA* but is instead dramatically stabilized in the presence of UmuD. On the basis of these observations, we suggest that formation of a UmuDC-RecA*-DNA quaternary complex protects the UmuDC proteins from proteolytic degradation and as a consequence helps to promote the switch from error-free to error-prone mechanisms of DNA repair.

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Section: Methodsmentioning

confidence: 99%

In vivo stability of the Umu mutagenesis proteins: a major role for RecA

Frank¹,

Gonzalez²,

Ennis³

et al. 1996

J Bacteriol

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“…Some reports have also described the genes responsible for quorum sensing, adhesion, and biofilm formation [147][148][149][150].…”

Section: Strains With Ability Of Biofilm Formationmentioning

confidence: 99%

The Use of Starter Cultures in Traditional Meat Products

Laranjo

Elías

Fraqueza

2017

Journal of Food Quality

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Starter cultures could play an essential role in the manufacture of traditional cured meat products. In order to achieve objectives related to meat products' quality and safety improvement, the selection of particular strains constituting a starter culture should be carried out in the context of its application, since its functionality will depend on the type of sausage and process conditions. Also, strain selection should comply with particular requirements to warrant safety. The aim of the current review is to update the knowledge on the use of starter cultures in traditional meat products, with focus on dry-fermented products. In this manuscript, we will try to give answers to some relevant questions: Which starter cultures are used and why? Why are LAB used? What are their role and their specific mode of action? Which other groups of microorganisms (bacteria and fungi) are used as starter cultures and how do they act? A particular revision of omics approach regarding starter cultures is made since the use of these techniques allows rapid screening of promising wild strains with desirable functional characteristics, enabling the development of starter cultures better adapted to the meat matrix.

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“…In addition, point mutations in the attenuators of erm(T) of Lactobacillus reuteri (246) or of erm(A) of S. aureus (265) or tandem duplications in the attenuators of erm(C) of S. aureus, Staphylococcus saprophyticus, and Staphylococcus equorum and of erm(A) of S. aureus (102,152,185,232,266) have been reported.…”

Section: Constitutive Expression Of Erm Genesmentioning

confidence: 99%

Modes and Modulations of Antibiotic Resistance Gene Expression

et al. 2007

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SUMMARY Since antibiotic resistance usually affords a gain of function, there is an associated biological cost resulting in a loss of fitness of the bacterial host. Considering that antibiotic resistance is most often only transiently advantageous to bacteria, an efficient and elegant way for them to escape the lethal action of drugs is the alteration of resistance gene expression. It appears that expression of bacterial resistance to antibiotics is frequently regulated, which indicates that modulation of gene expression probably reflects a good compromise between energy saving and adjustment to a rapidly evolving environment. Modulation of gene expression can occur at the transcriptional or translational level following mutations or the movement of mobile genetic elements and may involve induction by the antibiotic. In the latter case, the antibiotic can have a triple activity: as an antibacterial agent, as an inducer of resistance to itself, and as an inducer of the dissemination of resistance determinants. We will review certain mechanisms, all reversible, that bacteria have elaborated to achieve antibiotic resistance by the fine-tuning of the expression of genetic information.

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Molecular Characterization of a Plasmid-Borne (pGT633) Erythromycin Resistance Determinant (ermGT) from Lactobacillus reuteri 100-63

Cited by 136 publications

References 0 publications

In vivo stability of the Umu mutagenesis proteins: a major role for RecA

In vivo stability of the Umu mutagenesis proteins: a major role for RecA

The Use of Starter Cultures in Traditional Meat Products

Modes and Modulations of Antibiotic Resistance Gene Expression

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