Molecular characterization of a ssRNA mycovirus isolated from the forest pathogenic fungus Armillaria ostoyae

Tonka, Tomáš; Walterová, Lucie; Hejna, Ondřej; Čurn, Vladislav

doi:10.4149/av_2022_309

Cited by 5 publications

(3 citation statements)

References 6 publications

(14 reference statements)

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“…There are important forest pathogenic fungi, such as Cryphonectria parasitica , Phytophthora spp., Ophiostoma spp., Gremmeniella abietina , Hymenoscyphus fraxineus , Heterobasidion annosum and Armillaria spp., which host mycoviruses with diverse fungus-virus relationships [ 18 , 19 , 20 ]. In Armillaria , early studies by Blattny (1973) [ 21 ] and Reaves et al (1988) [ 22 ] described the presence of virus-like particles in A. mellea and A. ostoyae , but it was not until 2021 that viruses were confirmed by molecular characterization [ 13 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several positive- and negative-stranded ssRNA viruses and even two dsRNA mycoviruses have been described. Mitoviruses, ourmia-like viruses, tymoviruses, phlegiviruses, ambi-like viruses, several previously unclassified (+)ssRNA and two unclassified dsRNA viruses have been described in five different Armillaria species ( A. mellea , A. borealis , A. cepistipes , A. ostoyae , A. gallica ) [ 13 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic

Walterová,

Botella,

Hejna

et al. 2024

Viruses

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Members of the genus Armillaria are widespread forest pathogens against which effective protection has not yet been developed. Due to their longevity and the creation of large-scale cloning of Armillaria individuals, the use of mycoviruses as biocontrol agents (BCAs) against these pathogens could be an effective alternative. This work describes the detection and characterization of viruses in Armillaria spp. collected in the Czech Republic through the application of stranded total RNA sequencing. A total of five single-stranded RNA viruses were detected in Armillaria ostoyae and A. cepistipes, including viruses of the family Tymoviridae and four viruses belonging to the recently described “ambivirus” group with a circular ambisense genome arrangement. Both hammerhead (HHRz) and hairpin (HpRz) ribozymes were detected in all the ambiviricot sequences. Armillaria viruses were compared through phylogenetic analysis and confirmed their specific host by direct RT-PCR. One virus appears to infect both Armillaria species, suggesting the occurrence of interspecies transmission in nature.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic

Walterová,

Botella,

Hejna

et al. 2024

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are important forest pathogenic fungi, such as Chryphonectria parasitica, Phytophthora spp., Ophiostoma spp., Gremmeniella abietina, Hymenoscyphus fraxineus, Heterobasidion annosum and Armillaria spp., which host mycoviruses with diverse fungus-virus relationships [18][19][20]. In Armillaria, early studies by Blattny (1973) [21] and Reaves et al (1988) [22] described the presence of virus-like particles in A. mellea and A. ostoyae, but it was not until 2021, when viruses were confirmed by molecular characterization [13,23,24].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Characterization of Mycoviruses in the Genus <em>Armillaria</em> in the Czech Republic

Walterová,

Botella,

Hejna

et al. 2024

Preprint

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Members of the genus Armillaria are widespread forest pathogens against which effective protection has not yet been developed. Due to their longevity and the creation of large-scale cloning of Armillaria individuals, the use of mycoviruses as biocontrol agents (BCAs) against these pathogens could be an effective alternative. This work describes the screening of double-stranded RNA banding patterns as a first approach for potential virus discovery and the use of stranded total RNA-seq for viral detection and characterization in Armillaria spp. collected in the Czech Republic. A total of five single-stranded RNA viruses were detected in Armillaria ostoyae and A. cepistipes, including the first virus of the family Tymoviridae found infecting a member the genus Armillaria and four viruses belonging to the recently described virus group "ambiviruses" with circular ambisense genome organization. Both hammerhead (HHRz) and hairpin (HpRz) ribozymes were detected in all the ambiviricot sequences. Armillaria viruses were compared by phylogenetic analysis and confirmed their specific host by direct RT-PCR, which showed the occurrence of interspecies transmission.

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Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea

Erkmen,

Sahin,

Akata

2023

Virus Genes

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Molecular characterization of a ssRNA mycovirus isolated from the forest pathogenic fungus Armillaria ostoyae

Cited by 5 publications

References 6 publications

Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic

Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic

Molecular Detection and Characterization of Mycoviruses in the Genus <em>Armillaria</em> in the Czech Republic

Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea

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