Molecular characterization of a subgroup I geminivirus from a legume in South Africa.

Liu, L; Tonder, T van; Pietersen, Gerhard; Davies, Jeffrey W.; Stanley, John

doi:10.1099/0022-1317-78-8-2113

Cited by 65 publications

(48 citation statements)

References 21 publications

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“…Sequence analysis of the full-length amino acid sequence of the putative Rep of SsHADV-1 showed that it shares the highest sequence identity with the Reps of chickpea chlorotic dwarf virus (25), bean yellow dwarf virus (26), and tobacco yellow dwarf virus (27) (Table S2). These three viruses that infect dicotyledonous plants belong to the genus Mastrevirus (Fig.…”

Section: Resultsmentioning

confidence: 99%

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Fù

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

497

341

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Mycoviruses are viruses that infect fungi and have the potential to control fungal diseases of crops when associated with hypovirulence. Typically, mycoviruses have double-stranded (ds) or singlestranded (ss) RNA genomes. No mycoviruses with DNA genomes have previously been reported. Here, we describe a hypovirulenceassociated circular ssDNA mycovirus from the plant pathogenic fungus Sclerotinia sclerotiorum. The genome of this ssDNA virus, named Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), is 2166 nt, coding for a replication initiation protein (Rep) and a coat protein (CP). Although phylogenetic analysis of Rep showed that SsHADV-1 is related to geminiviruses, it is notably distinct from geminiviruses both in genome organization and particle morphology. Polyethylene glycol-mediated transfection of fungal protoplasts was successful with either purified SsHADV-1 particles or viral DNA isolated directly from infected mycelium. The discovery of an ssDNA mycovirus enhances the potential of exploring fungal viruses as valuable tools for molecular manipulation of fungi and for plant disease control and expands our knowledge of global virus ecology and evolution.

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Section: Resultsmentioning

confidence: 99%

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Fù

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

497

341

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“…Similarly, previously studied, tandem repeat infectious clones of (Stanley et al, 1986) and BeYDV-[ZA : Mpu : 94] (BeYDV; Y11023) (Liu et al, 1997).…”

Section: Hanleymentioning

confidence: 99%

Differential interaction between cassava mosaic geminiviruses and geminivirus satellites

Patil

Fauquet

2010

Journal of General Virology

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Geminiviruses are often associated with subviral agents called DNA satellites that require proteins encoded by the helper virus for their replication, movement and encapsidation. Hitherto, most of the single-stranded DNA satellites reported to be associated with members of the family Geminiviridae have been associated with monopartite begomoviruses. Cassava mosaic disease is known to be caused by viruses belonging to nine different begomovirus species in the African continent and the Indian subcontinent. In addition to these species, several strains have been recognized that exhibit contrasting phenotypes and infection dynamics. It is established that Sri Lankan cassava mosaic virus can trans-replicate betasatellites and can cross host barriers. To extend these studies further, we carried out an exhaustive investigation of the ability of geminiviruses, selected to represent all cassava-infecting geminivirus species, to trans-replicate betasatellites (DNA-b) and to interact with alphasatellites (nanovirus-like components; previously called DNA-1). Each of the cassava-infecting geminiviruses showed a contrasting and differential interaction with the DNA satellites, not only in the capacity to interact with these molecules but also in the modulation of symptom phenotypes by the satellites. These observations could be extrapolated to field situations in order to hypothesize about the possibility of acquisition of such DNA satellites currently associated with other begomoviruses. These results call for more detailed analyses of these subviral components and an investigation of their possible interaction with the cassava mosaic disease complex. INTRODUCTIONPlant viruses are often associated with satellite DNA molecules, which are, in most cases, able to modulate replication and symptom expression of their helper virus (Roossinck et al., 1992;Simon et al., 2004). These satellite molecules completely lack sequence identity to their helper viruses and depend on the helper virus for all or some of the following functions: replication, movement, encapsidation and transmission. Satellite molecules were initially reported to be associated with RNA viruses; these satellites are very well characterized (Simon et al., 2004). In the last decade, more than 500 satellite sequences associated with begomoviruses (family Geminiviridae) have been isolated from a diverse range of cultivated crops and weeds Briddon et al., 2008). Fauquet, unpublished data). In addition to these subviral components, geminiviruses have been shown to be accompanied by smaller-sized DNA molecules, called defective DNA molecules, that are derived from the helper virus genomes, but are not satellite molecules (Patil & Dasgupta, 2006;Simon et al., 2004). Alphasatellites are 1.3 kb nanoviruslike components that, in some cases, suppress viral disease symptoms. Although alphasatellites encode a replicationassociated protein, they depend on the helper virusencoded proteins for movement and encapsidation ). The first alphasatellite components identified were asso...

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“…(Morris et al, 1992;Horn et al, 1993). Albeit Bean yellow dwarf virus (BeYDV) (Liu et al, 1997a) (infect dicotyledonous plants) presence has been recorded in both Pakistan and South Africa (Halley-Scott et al, 2007). However, Thomas et al (2010) reported Chickpea chlorosis virus (CpCV-A, CpCV-B) and Chickpea redleaf virus (CpRLV) from Australia.…”

Section: Turnip Curly Topmentioning

confidence: 99%

Transmission Specificity and Coinfection of Mastrevirus with Begomovirus

Khalid¹,

Ur-Rehman²,

Hameed³

et al. 2017

IJAB

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Coat protein (CP) of geminivirus is not requisite for replication of viral DNA yet it is multifunctional and have crucial role in virus transmission by insect vector, systemic infection and virion development. Although in geminivirus, systemic infection rely both on function of CP and specific host-geminivirus-vector interaction but in bipartite begomoviruses CP is dispensable for systemic infection and progression of symptoms. Nevertheless its role in spreading systemic infection cannot be neglected in monopartite viruses' viz. mastrevirus, begomoviruses and curtoviruses, therefore any alteration in CP gene upshot in a new epidemiological adaptation hence worth to study. So the study was designed to present a better picture to view regarding coinfection within different Geminiviruses belonging to mastrevirus and begomovirus particularly due to vector inspecificity consequently resulted in revolutionary recombination events, which ultimately yield new viruses, have more drastic affects on crops. Moreover, co-evolution of both genera may open new horizons in understanding the complexity of cotton leaf curl disease and then to chalk out strategies to counter this emerging threat in Pakistan.

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Molecular characterization of a subgroup I geminivirus from a legume in South Africa.

Cited by 65 publications

References 21 publications

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus

Differential interaction between cassava mosaic geminiviruses and geminivirus satellites

Transmission Specificity and Coinfection of Mastrevirus with Begomovirus

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