Molecular Characterization of a Supergene Conditioning Super-High Vitamin C in Kiwifruit Hybrids

McCallum, John; Laing, William A.; Bulley, Sean; Thomson, Susan; Catanach, Andrew; Shaw, Martin; Kanebel, Mareike; Tahir, Jibran; Deroles, Simon C.; Timmerman-Vaughan, Gail M.; Crowhurst, Ross N.; Hilario, Elena; Chisnall, Matthew; Lee, Robyn; Macknight, Richard C.; Seal, Alan

doi:10.20944/preprints201906.0293.v1

Cited by 2 publications

(1 citation statement)

References 30 publications

(42 reference statements)

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“…Hence, human selection for larger fruit size, less acidity, and increased sweetness, has produced unwanted consequences (Fang et al, 2017). Obviously, the control of AsA content in crops is very attractive in breeding programs (Davey and Keulemans, 2004;Mellidou et al, 2012;Locato et al, 2013;Fenech et al, 2019;McCallum et al, 2019). Moreover, considerable interest exists for the study of the biochemical steps of biosynthesis and for the characterization of regulatory controls at different levels including those of degradation (Hancock, 2009).…”

Section: Discussionmentioning

confidence: 99%

L-Ascorbic acid in plants: from biosynthesis to its role in plant development and stress response

Viviani¹,

Fambrini²,

Giordani³

et al. 2021

Agrochimica

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L-Ascorbic acid (AsA; vitamin C), is a common antioxidant in nature. In plants, it plays an important role in photosynthesis as it eliminates reactive oxygen species (ROS) generated during electron transport. Unlike animals that use D-Glucuronate as a precursor, plants produce AsA by four alternative routes using Myo-inositol, L-Gulose, D-Mannose/L-Galactose and D-Galacturonate as main precursors. Humans cannot produce AsA due to the absence of the enzyme that catalyzes the last biosynthetic step, so they need plant sources. Epidemiological studies suggest that the consumption of AsA is important for health; therefore, increasing the vitamin C content of crops could be an important goal. Many approaches have been taken to increase the vitamin C content in plants, but challenges remain. Here, we examine the biosynthesis and recycling pathways of AsA, the close connection with AsA and abiotic and biotic stresses, as well as different strategies for increasing its content in plants.

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Section: Discussionmentioning

confidence: 99%

L-Ascorbic acid in plants: from biosynthesis to its role in plant development and stress response

Viviani¹,

Fambrini²,

Giordani³

et al. 2021

Agrochimica

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Deciphering the Genomic Characterization of the GGP Gene Family and Expression Verification of CmGGP1 Modulating Ascorbic Acid Biosynthesis in Melon Plants

Yang,

Amanullah,

et al. 2024

Antioxidants

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Ascorbic acid (AsA), also known as vitamin C, is a well-known antioxidant found in living entities that plays an essential role in growth and development, as well as in defensive mechanisms. GDP-L-galactose phosphorylase (GGP) is a candidate gene regulating AsA biosynthesis at the translational and transcriptional levels in plants. In the current study, we conducted genome-wide bioinformatic analysis and pinpointed a single AsA synthesis rate-limiting enzyme gene in melon (CmGGP1). The protein prediction analysis depicted that the CmGGP1 protein does not have a signaling peptide or transmembrane structure and mainly functions in the chloroplast or nucleus. The constructed phylogenetic tree analysis in multispecies showed that the CmGGP1 protein has a highly conserved motif in cucurbit crops. The structural variation analysis of the CmGGP1 gene in different domesticated melon germplasms showed a single non-synonymous type-base mutation and indicated that this gene was selected by domestication during evolution. Wild-type (WT) and landrace (LDR) germplasms of melon depicted close relationships to each other, and improved-type (IMP) varieties showed modern domestication selection. The endogenous quantification of AsA content in both the young and old leaves of nine melon varieties exhibited the major differentiations for AsA synthesis and metabolism. The real-time quantitative polymerase chain reaction (qRT-PCR) analysis of gene co-expression showed that AsA biosynthesis in leaves was greater than AsA metabolic consumption, and four putative interactive genes (MELO3C025552.2, MELO3C007440.2, MELO3C023324.2, and MELO3C018576.2) associated with the CmGGP1 gene were revealed. Meanwhile, the CmGGP1 gene expression pattern was noticed to be up-regulated to varying degrees in different acclimated melons. We believe that the obtained results would provide useful insights for an in-depth genetic understanding of the AsA biosynthesis mechanism, aimed at the development of improving crop plants for melon.

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Molecular Characterization of a Supergene Conditioning Super-High Vitamin C in Kiwifruit Hybrids

Cited by 2 publications

References 30 publications

L-Ascorbic acid in plants: from biosynthesis to its role in plant development and stress response

L-Ascorbic acid in plants: from biosynthesis to its role in plant development and stress response

Deciphering the Genomic Characterization of the GGP Gene Family and Expression Verification of CmGGP1 Modulating Ascorbic Acid Biosynthesis in Melon Plants

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