Molecular characterization of a teleost-specific toll-like receptor 22 (tlr22) gene from yellow catfish (Pelteobagrus fulvidraco) and its transcriptional change in response to poly I:C and Aeromonas hydrophila stimuli

Wei, Xiu-Ying; Wang, Jun; Guo, Shengtao; Lv, Yunyun; Li, Yanping; Qin, Chuanjie; Zou, Youlan; Shi, Qingchao; Hu, Ping; Xiong, Xiang; He, Ya‐Ling; Li, Rui; Huang, Ze-Jin; Chen, Dunxue; Wen, Zhengyong

doi:10.1016/j.fsi.2023.108579

Cited by 10 publications

(3 citation statements)

References 34 publications

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“…E. tarda , a Gram‐negative bacterium, causes substantial biomass and economic losses in aquaculture globally (Leung et al, 2012), seriously threatening the healthy development of fisheries. Studies have shown that when fish are infected with poly I: C, LPS, and E. tarda , the expression of immune‐related genes is activated (Kasthuriarachchi et al, 2021; Wei et al, 2023; Zhang et al, 2023). Seahorses have a unique immune system.…”

Section: Discussionmentioning

confidence: 99%

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Zhang,

Wang,

et al. 2024

J World Aquaculture Soc

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Abstractβ‐Catenin (CTNNB) is a core downstream mediator in the Wnt/β‐catenin signaling pathway, which plays a role in immune regulation and cell differentiation. In the present study, two CTNNB genes were identified by genomic analysis of Hippocampus erectus and named He‐CTNNB1 and He‐CTNNB2. He‐CTNNB1 was more conserved than He‐CTNNB2. The full length of He‐CTNNB1 was 2369 bp, including an open reading frame of 2275 bp that encoded 737 amino acids containing 12 armadillo repeats. Phylogenetic analysis revealed that He‐CTNNB1 clusters had homologs in other bony fishes. Tissue distribution analysis showed the highest mRNA expression of He‐CTNNB1 in the ovary, liver, and kidney of the lined seahorse under normal physiological conditions. The transcription level of He‐CTNNB1 was significantly increased in the liver and kidney in response to LPS, poly I:C, and Edwardsiella tarda challenge (p < 0.05). In vitro overexpression of He‐CTNNB1 significantly upregulated the expression of key genes of the canonical Wnt/β‐catenin signaling pathway, such as axin2, cMyc, and cyclinD (p < 0.05), which increased cell proliferation during the wound‐healing process. Taken together, our results suggest that He‐CTNNB1 is essential for cell proliferation and the innate immune response in H. erectus.

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Section: Discussionmentioning

confidence: 99%

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Zhang,

Wang,

et al. 2024

J World Aquaculture Soc

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“…The Toll pathway was first discovered as a developmental pathway in D. melanogaster (122) and it included signaling to nuclear factor kappa B (NF-kB), which is crucial for immunity and embryonic development (Figure 2) (123). The study of this pathway enables the characterization of toll-like receptors (TLRs), and by using this knowledge, it changed how we perceive the mammalian immune system (124).…”

Section: The Toll Signaling Pathwaymentioning

confidence: 99%

Insect-pathogen crosstalk and the cellular-molecular mechanisms of insect immunity: uncovering the underlying signaling pathways and immune regulatory function of non-coding RNAs

Mahanta,

Bhoi,

Komal

et al. 2023

Front. Immunol.

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Multicellular organisms are constantly subjected to pathogens that might be harmful. Although insects lack an adaptive immune system, they possess highly effective anti-infective mechanisms. Bacterial phagocytosis and parasite encapsulation are some forms of cellular responses. Insects often defend themselves against infections through a humoral response. This phenomenon includes the secretion of antimicrobial peptides into the hemolymph. Specific receptors for detecting infection are required for the recognition of foreign pathogens such as the proteins that recognize glucans and peptidoglycans, together referred to as PGRPs and βGRPs. Activation of these receptors leads to the stimulation of signaling pathways which further activates the genes encoding for antimicrobial peptides. Some instances of such pathways are the JAK-STAT, Imd, and Toll. The host immune response that frequently accompanies infections has, however, been circumvented by diseases, which may have assisted insects evolve their own complicated immune systems. The role of ncRNAs in insect immunology has been discussed in several notable studies and reviews. This paper examines the most recent research on the immune regulatory function of ncRNAs during insect-pathogen crosstalk, including insect- and pathogen-encoded miRNAs and lncRNAs, and provides an overview of the important insect signaling pathways and effector mechanisms activated by diverse pathogen invaders.

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“…Yellow catfish (Pelteobagrus fulvidraco) belongs to family Bagridae, order Siluriformes, and is an omnivorous freshwater fish that is widely cultured in China due to its rapid growth, good taste, and valuable nutrition values (Guo et al, 2023;Wei et al, 2023). Thus far, two key genes involved in HUFA biosynthesis including fad2 and elovl5 have been identified in yellow catfish (Song et al, 2015;Qin et al, 2017), but their exact roles have not been well investigated.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)

Zeng,

Wei,

Qin

et al. 2023

Front. Mar. Sci.

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Elongase of very long-chain fatty acid 8 (Elovl8) is a new member identified in the Elovl family that is involved in the synthesis of highly unsaturated fatty acids (HUFAs). However, the evolutionary and physiological roles of this enzyme are still largely unknown. In the present study, the elovl8 gene was identified and characterized from yellow catfish Pelteobagrus fulvidraco, and then its evolutionary and molecular characteristics as well as transcriptional changes in response to various nutritional status were determined. Results showed that the open reading frame (ORF) of elovl8 was 795 bp in length, encoding a protein of 264 amino acids. Multiple sequences alignment showed that the yellow catfish Elovl8 was highly conserved with other homologs in teleosts, sharing similar structural characteristics (including six conserved transmembrane α-helical domains, four conserved elongase motifs, and three highly conserved cysteine residues). Meanwhile, comparisons of genetic synteny confirmed that the elovl8 gene identified from the yellow catfish was the homolog of elovl8b in other teleosts, and thus, the elovl8a gene was lost in the genome of the yellow catfish. Gene structure analysis revealed that the elovl8b gene contained eight exons and seven introns, which was highly conserved in teleosts, implying the functional conservation among various fish species. Tissue distribution analysis detected by real-time quantitative PCR (RT-qPCR) showed that the elovl8 gene was extensively expressed in all detected tissues except eyes, with high expression levels in the intestine and liver. Temporal expression analysis revealed that the expression level of elovl8 was stably expressed in the early 12 h after fertilization, and then dramatically decreased at 24, 48, 72, and 96 h after fertilization, implying that elovl8 is required for HUFA biosynthesis in the early development stages. Functional experiments showed that the expression of the elovl8 gene was stimulated after feeding with egg yolk but was not obviously affected after feeding with halogenated worms, indicating that diets full of HUFAs can inhibit the expression of elovl8 in yellow catfish. Our findings will help us to better understand the evolutionary and functional characteristics of elovl8 in teleosts, and lay a solid basis for investigating the regulation mechanism of HUFA biosynthesis.

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Molecular characterization of a teleost-specific toll-like receptor 22 (tlr22) gene from yellow catfish (Pelteobagrus fulvidraco) and its transcriptional change in response to poly I:C and Aeromonas hydrophila stimuli

Cited by 10 publications

References 34 publications

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation

Insect-pathogen crosstalk and the cellular-molecular mechanisms of insect immunity: uncovering the underlying signaling pathways and immune regulatory function of non-coding RNAs

Molecular characterization, spatiotemporal expression patterns of fatty acid elongase (elovl8) gene, and its transcription changes in response to different diet stimuli in yellow catfish (Pelteobagrus fulvidraco)

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