2022
DOI: 10.15406/jcpcr.2022.13.00483
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Molecular characterization of a Tunisian B-lineage leukemia using Kde gene rearrangements 

Abstract: Background: Molecular approaches applied to the study of B-lineage leukemia have made significant contributions in understanding and characterizing the disease. The Kde rearrangement was targeted as clonal marker to characterize Tunisian B-chronic lymphocytic leukemia (B-CLL) and B-acute lymphoblastic leukemia (B-ALL).Objective: We used a multiplex PCR to amplify deletional Kde rearrangements and Southern blot assay with Cκ and Kde probes in seven B-CLL and two B-ALL. Direct sequencing was performed in order t… Show more

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